Method for quickly detecting mandelic acid content

A mandelic acid, content technology, applied in the field of biochemical analysis, to achieve the effect of high safety, simple equipment and low price

Inactive Publication Date: 2019-10-11
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned existing technical problems, the present invention provides a method for quickly detecting the content of mandelic acid, which avoids the technical problems in the prior art such as expensive detection instruments, complicated detection methods, and high detection requirements, and provides A detection method with simple operation, high precision, wide application, good selectivity and convenient use

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  • Method for quickly detecting mandelic acid content
  • Method for quickly detecting mandelic acid content
  • Method for quickly detecting mandelic acid content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Prussian blue colorimetric method measures different concentrations of mandelic acid

[0029] Take 0, 0.5, 1.0, 1.5, 2.0, 2.5mL 0.0657M mandelic acid standard solution in six 25mL volumetric flasks, add 5, 4.5, 4, 3.5, 3, 2.5mL of distilled water, and then add 10mM SDS solution in turn 2.0mL, 1.0mL of 0.03M ferric chloride solution, 1.0mL of 0.02M potassium ferricyanide solution, add 0.1M hydrochloric acid solution to constant volume, react for 20min, and scan at full wavelength. Such as figure 2 As shown, in the Prussian blue method, the absorption spectrum of the reaction system has a significant difference, the absorbance of the reaction system increases with the increase of the concentration of mandelic acid, and the absorption spectrum has a maximum absorption peak at 710nm, which is different from the absorption spectrum of Prussian blue The characteristics coincided, indicating that the reaction was carried out successfully, and proved the feasibil...

Embodiment 2 3

[0030] Embodiment 2 ferric chloride concentration optimization

[0031] Take 5mL of mandelic acid sample solution and add them to seven 25mL volumetric flasks, respectively add 2mL of 10mM SDS solution, add 1mL of 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07M ferric chloride solution, and then add 0.02MK 3 Fe[Fe(CN) 6 ] solution 1mL, and finally add 0.1M hydrochloric acid solution to volume. After reacting for 20 min, the absorbance was measured at 710 nm with a UV spectrophotometer. The results are shown in Table 1, with FeCl 3 As the concentration increases, the absorbance increases. When the concentration of ferric chloride increases to 0.06M, the absorbance tends to be stable. Therefore, the optimum concentration of ferric chloride solution is 0.06M.

[0032] The impact of the concentration of ferric chloride in table 1 on the absorbance value

[0033] Ferric chloride concentration (M) 0.01 0.02 0.03 0.04 0.05 0.06 0.07 OD 710

Embodiment 3

[0034] Embodiment 3 Potassium ferricyanide concentration optimization

[0035] Take 5mL sample solution and add them to seven 25mL volumetric flasks, add 2mL of 10mMSDS solution, 1mL of 0.06M ferric chloride solution, and 1mL of 0.0005, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035M potassium ferricyanide solution respectively , followed by adding 0.1M hydrochloric acid solution to volume. After reacting for 20 min, the absorbance was measured at 710 nm with a UV spectrophotometer. The results are shown in Table 2, with K 3 Fe[Fe(CN) 6 ] concentration increases, the absorbance first increases and then decreases, and reaches the highest value when the concentration of potassium ferricyanide is 0.002M. Therefore, the optimum concentration of potassium ferricyanide is 0.002M.

[0036] The influence of table 2 potassium ferricyanide concentration on the absorbance value

[0037] Potassium ferricyanide concentration (mM) 5 10 15 20 25 30 35 OD 710

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Abstract

The invention discloses a mehtod for quickly detecting mandelic content. The method comprises the following steps: firstly adding a sodium lauryl sulfate (SDS) solution into a to-be-detected sample, and then adding a ferric chloride solution, oscillating and uniformly mixing, and then adding a potassium ferricyanide solution, and determining an absorbance value at 710nm by using an ultraviolet spectrophotometer after sufficiently developing; computing the mandelic acid concentration according to the absorbance value, determining to obtain the relative standard deviation and recovery rate as 0.36% to 0.62% and 96.51% to 99.69%. The method disclosed by the invention is simple in operation, quick, safe, cheap and precise, and has a certain application value in the mandelic acid content detection; and meanwhile, the method is a high-flux screening method of the activity of the nitrilase taking mandelonitrile, 4-hydroxy mandelonitrile and 2-chlorine mandelonitrile as substrate.

Description

[0001] (1) Technical field [0002] The invention belongs to the field of biochemical analysis, and relates to a kind of mandelic acid, specifically a method for rapidly detecting the content of mandelic acid and its derivatives, and also a method for mandelonitrile, 4-hydroxymandelonitrile and 2-chloromandelonitrile A high-throughput screening method for nitrilase activity with nitriles as substrates. [0003] (2) Background technology [0004] Mandelic acid, also known as mandelic acid, as an important pharmaceutical intermediate, can be used to synthesize cephalosporins, antitumor agents, anti-obesity agents, etc. It is also an important chiral resolution agent. Mandelic acid was first extracted from peach leaves and willow leaves. It has the same source as salicylic acid. Mandelic acid has a significant analgesic effect. At present, methods for detecting mandelic acid have been reported, including high performance liquid chromatography (Food Industry, 2019, 40(03): 306-310...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 张新红王储炎赵海华徐涛
Owner HEFEI UNIV
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