169 results about "Mammary fluid" patented technology

Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with a solid phase sample collection medium, alternatively facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples to evaluate breast disease, including cancer. The biological sample may include whole cells or cellular components, purified or bulk proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Methods, kits and adapter devices relating to the breast pump device are also provided. Yet additional methods, devices, accessories, and materials are provided for laboratory handling and processing of breast fluid samples and for related diagnostic methods.

Breast pump for expressing milk from a breast, comprising at least one breast cup (2) for receiving a breast, a pumping system (10) in fluid connection (11) with the at least one breast cup (2) for applying a negative pressure on said breast and a detection unit (3) comprising at least one sensor for measuring a parameter during use of the breast pump (1), wherein the breast pump (1) is adapted for optimising breast pump settings by actively controlling at least one breast pump pumping property, based on measurements of the at least one sensor (4, 5), during a period of use of the breast pump (1), in order to personalise operation of said breast pump (1) for a particular user.

The present invention provides materials and methods for the production of recombinant BMPs in transgenic animals. In particular, the invention provides materials and methods for the production of recombinant BMPs in the milk of transgenic animals that express recombinant BMPs in the mammary gland.

An apparatus (1) for extracting milk from a breast, comprising means (2,3) for receiving at least a portion of a breast and its nipple; a container (5) for collecting the milk operatively connected to said means (2,3) for receiving at least partially a breast and its nipple; a pump unit (100) operatively connected to said means (2,3) for receiving at least partially a breast and its nipple, said pump unit (1009 being operated manually to generate at least an aspiration able to cause a suction on the breast by imposing a predetermined degree of vacuum within said means for receiving the breast and the nipple. The manually operated pump unit (100) comprises means for regulating the degree of vacuum that is generated. Pump unit (100) operated manually and connectable to a kit for collecting milk from a breast.

The invention belongs to the technical field of genetic engineering and transgenic engineering and in particular relates to a method for building mammary gland specific expression vectors of human thrombopoietin gene and goat beta-casein gene promoter. The vectors which are built are transferred to COS-7 and HC-11 cells and expressed on eukaryotic cell level verification hTPO. The built mammary gland specific expression vectors of the human thrombopoietin gene and the goat beta-casein gene promoter are applied to prepare a mammary gland bioreactor of transgenic animals and are expressed with high level in the milk of transgenic mammalian, and the vectors provide useful drug protein-hTPO for clinic. The mammary gland specific expression vectors of human thrombopoietin gene and goat beta-casein gene promoter which are provided by the invention provide a foundation for further research and development.

The invention provides an exosome separation method and a separation device thereof. The exosome separation method comprises the steps: firstly filtering or centrifuging blood, urea, saliva, cerebrospinal fluid, milk, ascetic fluid or amniotic fluid to remove impurities larger than 1 micrometer; then utilizing an even pore diameter anodized aluminum oxide film with the pore diameter correspondingto the diameter size of the exosome to filter, collecting filtrate or exosome on the even pore diameter anodized aluminum oxide film to obtain required exosome. The exosome separation device comprisesa membrane filter with the even pore diameter anodized aluminum oxide film as a filter membrane. By means of the separation method and the separation device thereof disclosed by the invention, the exosome can be simply and efficiently separated, the different sizes of exosome can be cut out and classified, and the different sizes of exosome can be respectively and correspondingly analyzed, researched and applied.

The invention provides a lactation-promoting and menstruation-inducing Chinese medicinal composition for puerperae, which can regulate the functions of promoting lactation, inducing menstruation, removing blood stasis, replenishing blood, nourishing qi and promoting body fluid of the puerperae comprehensively. According to the lactation-promoting and menstruation-inducing Chinese medicinal composition, anemone clematis stem, corn stigma, lettuce seed, dwarf lilyturf tuber and rose are used as a monarch medicine and have the effects of promoting blood circulation, regulating menstruation and promoting lactation; cassia bark, chestnut, medlar, cinnamomvine and loofah are used as a minister medicine and exert a secondary effect to solve the problem of galactostasis and realize the functions of dredging collaterals and inducing menstruation; carrot, pumpkin, glutinous rice, papaya and malt are used as an assistant medicine, have a coordinative effect in a formula or have a treatment effect of enhancing the monarch medicine and the minister medicine and inhibit the toxic and side effects of other medicines; and almond, Chinese date, prepared rehmannia root, turmeric and cowherb seed assists the medicines to exert the effects of promoting blood circulation, promoting lactation and blending the medicines. According to the formula, blood circulation-promoting and blood stasis-reducing medicines are supplemented on the basis of lactation promotion and menstruation induction, and the blood stasis-reducing medicines are combined with medicines for treating various accompanied symptoms, so that an effect of blending the medicines is achieved, and a reasonable formula of the Chinese medicinal composition for treating postpartum galactostasis, mammary swelling, acute mastitis, blood stasis pain, body deficiency and hypodynamia is formed.

A device, system, and method employ spectrometry to interrogate udder tissue or milk to diagnose clinical and sub-clinical mastitis.

The invention discloses cow feed and a preparation method thereof. The feed comprises alfalfa hay, pumpkins, rice straw, corn stalks, wheat straw, soya bean meal, rice bran, green stalks, maize germ meal, cottonseed meal, wheat bran, wheat middlings, sunflower seeds, DDGS, whole cottonseeds, carrot, choline chloride, potassium iodide, beer brewer grains, citric acid, magnesium sulfate, tricalcium phosphate, sodium selenite, cod-liver oil, salt, vitamin premix and microelement premix. The preparation method includes a breaking step, a blending step and a pelletizing step. The cow feed and the preparation method thereof have the advantages that the feed has the effects of benefiting qi, nourishing blood and promoting lactation, qi and blood deficiency type cow postpartum hypogalactia can be treated, breast plumpness and normal nipple development can further be promoted, milk is full, and the immunity of cows is improved; moreover, normal grow of cow mammary glands and other body organs can be well guaranteed; the cure rate is high, the feed becomes effective quickly, the course of treatment is short and no toxic and side effects exist.

Systems and methods with variable and customized functionality for pumping milk from a breast, wherein the milk is expressed from the breast under suction and milk is expulsed from the pumping mechanism to a collection container under positive pressure.

The present disclosure relates generally to a composition for feeding an infant. More particularly, the disclosure relates to a nutritional composition for feeding an infant comprising a stem-cell deficient first component and a second component comprising breast milk stem cells (BSCs). The second component is added to the first component, and the BSCs partially or fully replenish the stem-cell deficient first component with respect to BSCs. In some embodiments the first component is stem-cell deficient human breast milk and the second component comprises BSCs from the infant's mother's own milk. The compositions of the present disclosure have been developed primarily as a personalized, nutritional composition providing the beneficial effects of an infant's mother's own BSCs to the infant while not being able to receive the full benefits of being breastfed directly.

The present disclosure also relates to a composition for feeding an infant and to processes of their production. In particular, the disclosure relates to a composition for feeding an infant comprising a first and a second component, wherein the first component provides the majority of the nutritional value to said composition, and wherein the second component comprises at least one live bacterium from human breast milk or the lactating human mammary gland. The disclosure has been developed primarily as a composition for feeding a mother's own infant, wherein the composition comprises processed human breast milk with a substantially reduced live bacterial cell count as compared to unprocessed breast milk but which at least one live bacterium, from the mother's own breast milk, is added such as to partially or fully replenish the processed human breast milk with respect to the at least one live bacterium. More particular, the composition is a personalised composition for feeding the mother's own preterm infant.

The present invention also related to the combination of BSC and bacterial replenishment as described herein.

The invention provides a food for promoting lactation and a preparation method of the food. The food is prepared from the following raw materials in parts by weight: 500-750 parts of wild live crucian, 100-150 parts of peanuts, 100-150 parts of millet. 100-150 parts of phaseolus calcaratus, 100-150 parts of red beans, 80-100 parts of black beans, 20-30 parts of luffa, 20-30 parts of beef, 20-30 parts of black sesame, 10-15 parts of longan, 10-15 parts of cinnamon and 10-15 parts of Chinese dates. The food and the preparation method provided by the invention have the advantages that great retention, release and maximum absorption and utilization of nutrition of the raw materials for a human body are realized, the effect is rapid, the functions of tonifying qi and blood, strengthening spleen and stomach, relieving liver depression, regulating yin and yang, nourishing blood and yin, invigorating and promoting lactation can be achieved for a puerpera, and the food not only can help the postpartum breast milk secretion, but also is beneficial to generating essence, nourishing blood, and promoting lactation, can help regulation of postpartum insufficiency of qi and blood and inappetence, and can prevent the postpartum breast diseases and promote the restoration of a puerpera body.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The invention relates to the technical field of breast pumping articles, and particularly relates to a breast pump. The breast pump comprises a breast milk collector, a pumping source and a breast milk pumping shield, wherein the pumping source is provided with a relief port and an air suction port; the breast milk pumping shield is provided with a relief passageway and a breast milk outlet; the breast milk outlet is communicated with the breast milk collector through a first hose; the breast milk collector is communicated with the air suction port of the pumping source through a second hose; the relief passageway is communicated with the relief port of the pumping source through a third hose; when the breast pump is used, the breast milk pumping shield covers the breasts and does not need to be held by hands as the breast milk pumping shield is separated from the breast milk collector; the pumping source is started for pumping, and air pressure in the breast milk collector and the breast pumping shield turns to be negative pressure; and when the air pressure is lower than the pressure in the breasts, breast milk is pumped out from the nippers into the breast milk pumping shield, and then the pumping source relieves, the pressure in the breast milk pumping shield is increased instantly after relief, the pressure of the breast milk collector is still in the negative pressure state, and the breast milk rapidly flows to the breast milk collector from the breast milk pumping shield through the first hose under the function of the pressure difference, so as to achieve the effect that no residual breast milk is left.