65 results about "Liver microsomes" patented technology

The present invention related to safe plant drug for treatment of liver disease, specifically, this invention proves a safe plant drug Schisandrin and its preparation. Schisandrin has the following pharmaceutical functions: increasing tumor suppresson genes express activity, decreasing activity of oncogenes, increasing immune function, increasing liver DNA synthesis, decreasing serum alamine aminotransferase activity, increasing glutathione level, increasing glutathione reductase activity, decreasing lipid peroxidation of liver, increasing hepatic microsomal monooxygenases activity, increasing ATP content in liver, increasing energy metabolism activity, decreasing density lipoprotein oxidation, protecting gastrointestinal function, increasing killer cell activity, increasing complement activity, decreasing induced liver cancer activity and decreasing grown of cancer cells.

The present invention is directed to a 2,4-bis(trifluoroethoxy)pyridine compound represented by formula (1):(wherein X1 represents a fluorine atom or a hydrogen atom) or a salt thereof, and to a drug containing the compound or the salt as an active ingredient.The compound has metabolic resistance in human liver microsome, good absorbability upon oral administration, and excellent ACAT inhibitory activity.

The invention discloses a method for liver microsomes to metabolize chlorinated paraffin in vitro, which comprises the following steps: (1) dissolving chlorinated paraffin in an organic solvent to obtain a chlorinated paraffin solution, adding serum to it, and obtaining Substrate system; (2) Add potassium phosphate buffer solution, liver microsomes and NADPH enzyme regeneration system to the substrate system successively, shake well to obtain the final reaction system, and then place it in a 37°C water bath for shaking reaction; (3) Add to the step Add ice-cold organic solvent to the reaction system of (2) to stop the reaction, then carry out centrifugation, take the supernatant, and measure the content of chlorinated paraffin. The invention adopts serum as the carrier of chlorinated paraffin, promotes the reaction between chlorinated paraffin and liver microsome, significantly improves the metabolic clearance rate of chlorinated paraffin, and realizes the determination of the metabolic clearance rate of liver microsome in vitro metabolism of chlorinated paraffin.

The invention relates to an LC-MC method used for determining NNK metabolites in liver microsomes and specifically to an LC-MC method used for analyzing and determining 4-(methyl nitroso)-1-(3-pyridyl)-1-butanone (NNK) metabolites in liver microsomes, which belongs to the field of biochemical analysis. The method provided in the invention mainly comprises the following steps: (1) preparation of an incubation system for liver microsomes; (2) sample treatment; (3) sample analysis and determination of NNK metabolites in the liver microsomes by using the LC-MC method. The invention has the following advantages of simple pre-treatment, a fast analysis speed, good selectivity, high detection sensitivity and capacity of analyzing and comparing the metabolism of NNK in different kinds of liver microsomes, can lay a methodological foundation for further research on in-vivo and in-vitro metabolism of NNK and other nitrosamine compounds and has critical significance.