69 results about "Intracellular calcium ion" patented technology

The invention relates to an amantadine nitrate compound with a neuroprotective effect, and preparation therefor and medical application thereof. The compound has a structure of a general formula (I) as shown in the specification, has multimechanisms, comprises NMDA receptor inhibition, NO releasing and inhibition of inward flow of calcium ions, and has a good protection effect on cells, particularly on neurons. The compound can be used for preparing drugs with a cytoprotection effect, and the drugs are used for preventing or treating diseases related to increasing of NMDA receptors, calcium ions in cells and the like, such as diseases related to neurodegeneration including senile dementia, parkinson's disease, brain stroke, glaucoma and the like, diseases related to a cardio-cerebrovascular system including parkinsonism combined with cerebral arteriosclerosis, and respiratory tract infectivity caused by influenza viruses.

A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.

The invention provides American ginseng milk and a preparation method thereof, and relates to a milk prepared product. The American ginseng milk is mixed by American ginseng extracting solution, milk with thickening agent and the like. The invention has the beneficial effects that the brain fatigue can be resumed, the brain insenescence can be delayed, the lipid metabolism can be mediated, the blood cholesterol can be reduced, the unavailable blood cholesterol reduction function caused by the low-choline content in new milk can be recovered, the kinetic fatigue damage caused by free radicals can be avoided, and the absorbing and the blocking of the calcium ions on the cell membrane can be adjusted, so that the side effects caused by the accumulation of the calcium ions in the cells can be avoided.

The invention discloses a 1,3,4-oxadiazole derivative and a synthetic method thereof, and an application of the derivative serving as a fluorescent indicator to detection of intracellular calcium ions (Ca<2+>). The method comprises the following steps of: under the protection of inert gas, performing a wittig reaction on the 1,3,4-oxadiazole derivative and a calcium ion response group to obtain a crude product; and concentrating the crude product, and separating through a silica gel column to obtain a pure product. The 1,3,4-oxadiazole derivative has the advantages of high sensitivity and high selectivity on Ca<2+>, large Stocks displacement, proportional measurement and the like; the color of the indicator changes greatly before and after bonding with Ca<2+>, so that identification and judgment can be performed conveniently through naked eyes; and moreover, the indicator can be loaded into cells, so that an intracellular Ca<2+> signal and the concentration thereof can be detected conveniently under a laser confocal microscope.

The present disclosure provides a light-activated, calcium-gated polypeptide; and a system comprising: a) the light-activated, calcium-gated polypeptide; and b) a fusion protein comprising a calcium responsive polypeptide and a protease that cleaves a proteolytically cleavable linker present in the light-activated, calcium-gated polypeptide. The present disclosure provides nucleic acids encoding the light-activated, calcium-gated polypeptide or the system, and cells comprising the nucleic acids. The present disclosure provides methods of detecting a change in intracellular calcium ion concentration. The present disclosure provides methods of controlling or modulating an activity of a cell.

A regulator for regulating physiological functions, such as activity of increasing an intracellular calcium ion concentration, activity of promoting growth hormone secretion, activity of promoting eating, regulatory activity relating to fat accumulation, activity of ameliorating heart function and activity of stimulating gastric acid secretion, of ghrelin, which regulator comprises a fatty acid of carbon number 2-35 or its derivative, and use thereof.

A fluorescent probe for calcium ion detection that has an excellent photofading resistance and quick Ca²⁺ detection kinetics and can be localized at an arbitrary site in a cell is provided. The fluorescent probe contains a compound represented by the following general formula (I) or a salt thereof:

A method for detecting intracellular calcium ions including (a) introducing the compound above or a salt thereof into a cell and (b) measuring the fluorescence emitted by the compound or a salt thereof in the cell is also provided.

The invention discloses a Panx3 gene overexpression red fluorescence lentiviral vector and a building method and application of the Panx3 gene overexpression red fluorescence lentiviral vector, and belongs to the field of gene engineering. According to the vector, a lentiviral vector pLL3.7 is used as a skeleton vector, a Panx3 gene, CMV promoter and RFP gene segment is inserted behind a CMV promoter of pLL3.7 in the direction from the 5' end to the 3' end, and the sequence of the segment is shown in SEQIDNO.1. The built Panx3 gene overexpression red fluorescence lentiviral vector has the advantages that the mutual interference problem of the overexpression vector and function research in Panx3 gene research is solved, a CMV serves as the promoter of the vector, eukaryon gene efficient expression can be guaranteed, RFP serves as a marker, the role of the Panx3 gene in intracellular calcium and ATP change after a half-channel is formed can be directly observed, the cell function is eventually affected, and a good tool is provided for research on the functions of the Panx3 gene.

A 3,4-dihydroquinazoline derivative or a pharmaceutically acceptable salt thereof and a composition containing the same are provided. The 3,4-dihydroquinazoline derivative or a pharmaceutically acceptable salt thereof show activities of blocking an intracellular influx of calcium ions acting as a secondary signal essential to the proliferation and growth of cancer cells, thereby inducing the cell cycle arrest of cancer cells, and eventually reinforcing the efficacy of existing anti-cancer drugs. A combination comprising the above compound and another anti-cancer drug is also provided.

The invention provides a preparation method for responsive nanoparticles with a mitochondrion-targeted function. The method comprises the steps: S1) mixing an inorganic calcium salt, dopamine and an alcohol solvent, so as to obtain a precursor solution; and S2) placing the precursor solution and ammonium bicarbonate in a closed environment in a noncontact manner, and carrying out heating for a reaction, so as to obtain the responsive nanoparticles. Compared with the prior art, the responsive nanoparticles prepared by the method has the advantages that the responsive nanoparticles are uniform in shape and uniform in size, have relatively high thermal stability and chemical stability, can achieve excited response of a variety of light of different wavelengths, can continuously generate calcium ions in tumor cells to increase a concentration of the calcium ions in the cells, and can also cooperate with micromolecular drugs to inhibit a calcium ion discharging action of a mitochondrion andcooperate with the micromolecular drugs to jointly destroy functions of the mitochondrion so as to promote damage of the mitochondrion, and thus, the aim of tumor therapy is achieved, so that the responsive nanoparticles have great potentials in cancer therapy in the future.