Responsive nanoparticles with mitochondrion-targeted function, preparation method therefor and application of responsive nanoparticles
A nanoparticle and responsive technology, applied in the field of nanomaterials, can solve the problems of single function of mitochondria and limited damage of mitochondria, and achieve the effects of inhibiting calcium ion excretion, synergistic treatment of mitochondria, high thermal stability and chemical stability
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[0035] The invention provides a method for preparing responsive nanoparticles with mitochondrial targeting function, comprising: S1) mixing inorganic calcium salt, dopamine and alcohol solvent to obtain a precursor solution; S2) mixing the precursor solution with carbonic acid Ammonium hydrogen is placed in a closed environment without contact with each other, and heated to react to obtain responsive nanoparticles.
[0036] Among them, the present invention has no special limitation on the types of all raw materials, as long as they are commercially available.
[0037] The inorganic calcium salt, dopamine and alcohol solvent are mixed to obtain a precursor solution; the inorganic calcium salt is preferably calcium chloride and / or calcium nitrate; the alcohol solvent is preferably ethanol; the mass ratio of the inorganic calcium salt to dopamine Preferably (10-20): 1, more preferably (12-18): 1, more preferably (14-16): 1, most preferably 15: 1; the ratio of the inorganic calci...
Embodiment 1
[0053] (1) Weigh 150 mg of calcium chloride, 10 mg of dopamine, and 20 mg of curcumin and add them to a beaker with 100 ml of ethanol for dissolution.
[0054] (2) Weigh 10 mg of cisplatin and dissolve it in 2 ml of DMSO, and disperse the solution into the above (1) beaker A.
[0055] (3) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.
[0056] (4) Place the A and B beakers in a vacuum desiccator at 40° C., and react for 10 h.
[0057] (5) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACaPtCur nanoparticles.
[0058] (6) Disperse the PDACaPtCur nanoparticles in (5) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine 800), and stir overnight.
[0059] (7) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanopar...
Embodiment 2
[0077] (1) Weigh 150 milligrams of calcium chloride and 10 milligrams of dopamine into a beaker containing 100 milliliters of ethanol for dissolution.
[0078] (2) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.
[0079] (3) Place the A and B beakers in a vacuum desiccator at 40° C., and react for 10 h.
[0080] (4) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACa nanoparticles.
[0081] (5) Disperse the PDACa nanoparticles in (4) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine is 800), and stir overnight.
[0082] (6) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanoparticles, which are designated as PEICa nanoparticles.
[0083] (7) Disperse the PEICa nanoparticles in (6) in 30 ml of ethanol, add 10...
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