55 results about "Hepatocyte function" patented technology

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

The present invention relates to a viral liver disease treating pharmaceutical composition, and discloses a traditional Chinese medicine composition, which is prepared from cape jasmine, tuckahoe, salvia miltiorrhiza, cortex lycii, radix pseudostellariae and amomum villosum lour through a suitable preparation method. According to the present invention, the traditional Chinese medicine compositionhas advantages of low raw material cost, easily-available raw materials and simple preparation method, and can significantly reduce the viral amount of viral hepatitis and improve the function of liver cells.

The invention discloses liver-protecting nutritional composition which comprises, by weight, 18-22 parts of pawpaw superfine powder, 8-12 parts of giant knot weed superfine powder, 8-12 parts of jujube superfine powder, 7-12 parts of hawthorn superfine power, 5-15 parts of tuchahoe powder, and 3-8 parts of Tengcha superfine powder. Formulation of the liver-protecting nutritional composition is reasonable. As being made of superfine powders, the liver-protecting nutritional composition has greatly reserved biological activity. Human bioavailability is improved. Since the liver-protecting nutritional composition is utilized for preparing the liver-protecting national food, hepatocyte function of patients with liver disease can be conditioned effectively, catabolism of fat granule is improved, liver microenvironment is improved remarkably, necrotic tissue regeneration is improved, catabolism load of the liver is released, and rehabilitation is improved after the patients with liver diseases eat the liver-protecting national food.

The invention discloses compound astragalus polysaccharide injection used for pigs and a preparation method thereof. Every 100L of compound astragalus polysaccharide injection used for the pigs contains the following raw materials, such as 0.5-5kg of astragalus polysaccharide, 1-10kg of lincomycin, 5-30kg of analginum, 1-10kg of inosine, 3-6L of 2-hydroxyl ethylamine and the like, wherein the astragalus polysaccharide can accelerate the lymphocyte to be converted and increased in value and accelerate the phagocytic function of macrophage; the lincomycin has strong antibiosis efficacy; the astragalus polysaccharide and the lincomycin are organically combined to perform an antiviral function, widen an antibacterial spectrum and enhance a targeted action on bacteria; the analginum has various functions of resisting bacterium, diminishing inflammation, carrying out antipyretic action, relieving pain and the like; and the inosine is favorable for restoring the function of a damaged hepaticcell. Above medicines have a reasonable formula, can simultaneously carry out symptomatic treatment and etiological treatment by aiming at diseases, has quick curative effect on mixed infection of pig virus and bacteria and treats both principal and secondary aspect of the disease, the medicine resistance of the pathogenic microorganism is lowered, the clinic symptom of a diseased pig is alleviated, and therefore the pig is quickly restored.

The present disclosure provides a culture medium formulation comprising human serum that can enhance functions of primary human hepatocytes, improve morphology, promote bile canaliculi formation and extend hepatocyte functional lifetime in vitro for over 10 weeks as compared to ˜3-4 weeks when using a conventional culture medium containing serum from bovine sources. The provided long-term culture model can be used to screen drugs for their efficacious and/or toxic effects over several weeks, improve drug-transporter assays via the larger bile canaliculi network, and to model several chronic liver diseases such as hepatitis, type 2 diabetes, malaria, liver fibrosis, liver cancer, and fatty liver disease.

The invention discloses a technical method for co-cultivating hepatocytes and hepatic sinusoidal endothelial cells and application of the technical method. The technical method is characterized in that a hepatic sinusoidal endothelial cell line is subjected to anaerobic cultivation for a certain period of time, and then the hepatic sinusoidal endothelial cell line is harvested and co-cultured witha hepatocyte line at a certain ratio. Co-culture with the hepatic sinusoidal endothelial cell line can partially simulate a liver environment in a human body, hepatocyte functions are facilitated, after the hepatic sinusoidal endothelial cells are activated by hypoxia, the ability of promoting the hepatocyte functions is enhanced, and no exogenous endothelial cell activator is needed, the introduction of exogenous materials is reduced, and subsequent cell application is facilitated. The invention further provides the application of the co-culture method in fields including drug screening, bioartificial liver preparation and the like.

With the present invention, liver cell function research of a human fetal liver cell line L-02 is performed, and the human fetal liver cell line L-02 is adopted in liver cell transplantation. The research results show that: the L-02 cells have good liver cell functions, can express a plurality of liver cell specificity factors, wherein the liver cell specificity factors comprise albumin, bilirubin glucuronosyltransferase, blood coagulation factors, transaminase and the like. 90% hepatectomy-induced acute liver failure (ALF) rat model experiment results show that: the survival time of the ALF rats can be effectively prolonged with the L-02 cells, the tumorigenesis risk does not exist, the L-02 cells is expected to be the good cell source for liver cell transplantation, bioartificial liver, medical researches related to the liver cell functions, and the like, and is applicable for treatment of the ALF, liver cell function researches, and other medical fields.

The present invention belongs to the field of medicine technology, and is especially a kind of medicine composition and its preparation process. The medicine composition is prepared with kurarinone 4-400 weight portions, glutathione 3-300 weight portions, and tiopronin 1-100 weight portions. The medicine composition may be prepared into different pharmaceutically acceptable forms, preferably injection and oral preparation. It has the pharmacological activities of antagonizing virus, improving liver cell function, antagonizing liver fibrosis, regulating immunity, diminishing inflammation, etc. and may be used in treating various hepatic diseases, viral hepatosis, medicinal hepatosis, alcoholic hepatosis, etc.

The invention discloses a method for preparing immortalized hepatocytes with reversible liver functions and application of the immortalized hepatocytes, and relates to the field of biomedicine. A Tet-on system is applied to the hepatocytes, and the functions of the hepatocytes are enhanced through the Tet-on system. The Tet-on system has the characteristics of high efficiency, accuracy, safety and reversibility. Therefore, a novel immortalized hepatocyte is prepared, reversible regulation and control of hepatocyte functions can be realized by adding or removing tetracycline analogues, and reversible enhancement of the hepatocyte functions is promoted. The preparation method is simple and easy to implement, the culture cost is low, seed cells are derived from immortalized hepatocytes, and clinical transformation is easy.

The invention discloses a hepatocyte culture solution which comprises dexamethasone with the final concentration of 1-20 [mu] M, curcumin with the final concentration of 1-20 [mu] M and a basic culture medium, the hepatocyte culture solution has the advantages of being simple in component and low in cost, hepatocytes with enhanced functions are obtained through quantitative induction, and application of the hepatocytes to treatment of liver diseases is accelerated. According to the culture method for enhancing the functionality of the hepatocytes and the used hepatocyte culture solution, sufficient nutrition can be provided for growth of the target hepatocytes, and expression of related metabolic enzymes and albumin genes of the target cells can be induced.

The invention discloses a hepatocyte in-vitro co-culture system as well as a construction method and application thereof. The hepatocyte in-vitro co-culture system comprises an extracellular matrix, hepatocytes and 3T3-J2 fibroblasts, wherein the hepatocytes are planted on the extracellular matrix, and the 3T3-J2 fibroblasts are laid on the surfaces of the hepatocytes. The construction method of the hepatocyte in-vitro co-culture system comprises the following steps: inoculating 3T3-J2 fibroblasts on a temperature-sensitive culture dish, carrying out culturing and stripping to obtain the hepatocyte in-vitro co-culture system. The hepatocyte in-vitro co-culture system provided by the invention provides a three-dimensional form closer to an in-vivo liver tissue microenvironment for human hepatocytes, ensures tight hepatocyte/hepatocyte interconnection and hepatocyte/supporting cell interaction, and can effectively enhance and maintain hepatocyte functions; and the system can be used forlong-term drug evaluation.

The invention relates to a manufacture method for biology artificial liver biology reactor that includes the following steps: expanding stem cell expanding to 1*10^7 OM, adding into cultivating liquid for suspensoid after assimilating and centrifuging, adding into hollow fiber filterer, and laying into incubator to take continuing expansion and changing inducing type cultivating liquid, the stem cell could have the cell having liver cell function. The invention has stable stem cell resource, and could save cost. It has little immunity source polarity, long time keeping activity, and is benefit for cure.

The invention discloses a traditional Chinese medicine composition for clearing away heat and toxic materials and removing stasis, and a preparation method thereof. The composition includes involucrate stahlianthus rhizome, rose rhodobryum herb, tangerine seeds, Chinese honey bee larva, bark of elm tree and persimmon leaf. The composition can enhance organism adaptation and digestive organ functions, improve insufficiency of the spleen and promote tissue metabolism; the composition has the efficacy of clearing away heat and toxic materials and removing stasis; in animal experiments, high and low dose groups have a certain inhibition effect on liver tumor, the physiological status of mice is good, and the survival rates of 45 days are respectively 100% and 80%; the composition has no toxicand side effects on human bodies, and can strengthen nutrition, inhibit liver tumor, repair hepatocyte functions, reverse liver cirrhosis and reduce the mortality of severe hepatitis; and the composition has synchronous therapeutic effects on complications of the liver tumor.