Culture method for enhancing functionality of hepatocytes and used hepatocyte culture solution

A technology of hepatocytes and culture medium, applied in cell culture active agent, culture process, animal cells and other directions, can solve the problems of difficulty in human primary hepatocytes, short in vitro proliferation cycle, etc. simple effect

Active Publication Date: 2021-10-29
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the in vitro proliferation cycle of these cells is short and the supply is limited, so it is difficult to directly apply human primary hepatocytes

Method used

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  • Culture method for enhancing functionality of hepatocytes and used hepatocyte culture solution
  • Culture method for enhancing functionality of hepatocytes and used hepatocyte culture solution
  • Culture method for enhancing functionality of hepatocytes and used hepatocyte culture solution

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Embodiment Construction

[0030] The present application will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] This application provides a hepatocyte culture medium, which is used to induce hepatocytes with a lower degree of differentiation, to enhance the specific function of hepatocytes, and to make the physiological state of induced hepatocytes closer to the physiological state of hepatocytes in the human body State, or close to the physiological state of human primary hepatocytes, to improve the possibility of clinical application.

[0032] The hepatic cell culture medium of the present application comprises the following components: dexamethasone with a final concentration of 1-20 μM, curcumin with a final concentration of 1-20 μM and basal medium.

[0033] The basal medium is selected from one of DMEM / F12 cell culture medium, F12 cell culture medium and William's E cell culture medium. DMEM / F-12 (Dulbecco's Modified Eagle Mediu...

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Abstract

The invention discloses a hepatocyte culture solution which comprises dexamethasone with the final concentration of 1-20 [mu] M, curcumin with the final concentration of 1-20 [mu] M and a basic culture medium, the hepatocyte culture solution has the advantages of being simple in component and low in cost, hepatocytes with enhanced functions are obtained through quantitative induction, and application of the hepatocytes to treatment of liver diseases is accelerated. According to the culture method for enhancing the functionality of the hepatocytes and the used hepatocyte culture solution, sufficient nutrition can be provided for growth of the target hepatocytes, and expression of related metabolic enzymes and albumin genes of the target cells can be induced.

Description

technical field [0001] The present application relates to the field of cell culture, in particular to a culture method for enhancing the functionality of hepatocytes, and the hepatocyte culture medium used. Background technique [0002] Liver transplantation is the most effective treatment for patients with end-stage liver failure, but the shortage of donors and high cost of treatment limit the application of this treatment. Following liver transplantation, hepatic cell transplantation technology has been proposed as another effective treatment for liver failure, and it can also be used as a bridging treatment for patients with acute liver failure leading to liver transplantation until a donor liver is available. Primary human hepatocytes have excellent synthesis and detoxification functions, etc., and are often used as an important cell source for liver transplantation. However, the in vitro proliferation cycle of these cells is short and the supply is limited, so it is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/067C12N2500/76C12N2501/30
Inventor 高毅黎少欧楚澎彭青
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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