52 results about "Diamine oxidase" patented technology

The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

LSD1, a homolog of nuclear amine oxidases, functions as a histone demethylase and transcriptional co-repressor. LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription. Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant de-repression of target genes, suggesting that LSD1 represses transcription via histone demethylation. The results thus identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histone methylases and demethylases.

The invention relates to a method for calculating tolerance dosage of sugar alcohol and function sugar of the human body. Xylitol is taken as an example, and the method comprises the following steps that mice with the volume of 200 g are selected as experimental animal objects, the mice with the volume of 200 g are divided into six experimental groups and one control group, and there are ten numbered mice of each group; the mice of six experimental mice are fed with the xylitol of different dosages in a filling mode every day; the mice of the control group are fed with saccharose in the filling mode; sufficient drinking water and ordinary feed are provided for the experimental mice; the mice are checked every night, and the diarrheal mice are counted; the feeding mode lasts for 14 days; the maximum tolerated dosage of the xylitol of the mice of each group is obtained according to diarrhea conditions of the mice on the last day, intestinal segment slice images of all the mice and the result of content of diamine oxidase; according to a drug dosage reduction formula between the experimental animal and the human, the maximum tolerated dosage of the mouse is converted to obtain the tolerance dosage of the xylitol of the human body. In an animal experiment mode, the range of the tolerance dosage of the sugar alcohol and the function sugar of the human body is obtained more accurately.

The present invention provides pharmaceutical compositions comprising droxidopa alone, or in combination with one or more further active ingredients, for the treatment of conditions, such as mood disorders, sleep disorders, or attention deficit disorders. In certain embodiments, the compositions useful in the methods of the invention comprise droxidopa and a compound selected from the group consisting of DOPA decarboxylase inhibiting compounds, catechol-O-methyltransferase inhibiting compounds, cholinesterase inhibiting compounds, monoamine oxidase inhibiting compounds, norepinephrine reuptake inhibiting compounds, selective serotonin reuptake inhibiting compounds, tricyclic antidepressant compounds, serotonin norepinephrine reuptake inhibiting compounds, norepinephrine dopamine reuptake inhibiting compound, noradrenergic and specific serotonergic antidepressants, and combinations thereof. The inventive compositions are particularly useful in the treatment of depression, narcolepsy, insomnia, and Attention Deficit / Hyperactivity Disorder (AD / HD).

The invention provides a kit for detecting the barrier function of the intestinal tract. The kit comprises a reaction device, one or more colorimetric solutions and one or more dilution solutions; the reaction device comprises a blood plasma separator and a reaction substrate, and the reaction substrate includes a blank hole, a diamine oxidase reaction hole, a D-lactic acid reaction hole and a bacterial endotoxin reaction hole; a blood plasma separating film is arranged in each of the holes of the blood plasma separator; and a blank pad is arranged in the blank hole.