51 results about "Diamine oxidase" patented technology

The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

LSD1, a homolog of nuclear amine oxidases, functions as a histone demethylase and transcriptional co-repressor. LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription. Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant de-repression of target genes, suggesting that LSD1 represses transcription via histone demethylation. The results thus identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histone methylases and demethylases.

The invention relates to a method for calculating tolerance dosage of sugar alcohol and function sugar of the human body. Xylitol is taken as an example, and the method comprises the following steps that mice with the volume of 200 g are selected as experimental animal objects, the mice with the volume of 200 g are divided into six experimental groups and one control group, and there are ten numbered mice of each group; the mice of six experimental mice are fed with the xylitol of different dosages in a filling mode every day; the mice of the control group are fed with saccharose in the filling mode; sufficient drinking water and ordinary feed are provided for the experimental mice; the mice are checked every night, and the diarrheal mice are counted; the feeding mode lasts for 14 days; the maximum tolerated dosage of the xylitol of the mice of each group is obtained according to diarrhea conditions of the mice on the last day, intestinal segment slice images of all the mice and the result of content of diamine oxidase; according to a drug dosage reduction formula between the experimental animal and the human, the maximum tolerated dosage of the mouse is converted to obtain the tolerance dosage of the xylitol of the human body. In an animal experiment mode, the range of the tolerance dosage of the sugar alcohol and the function sugar of the human body is obtained more accurately.

There is provided by this invention a simple and rapid amperometric biosensor for determining the level of histamine in seafood or fish. The biosensor combines the technology of screen-printing with immobilized diamine oxidase as the bioreceptor. IQ one embodiment of the present invention, the biosensor incorporates potassium hexacyanoferrate (III) as a mediator.

The invention discloses a process for producing 20 percent amitraz emulsifiable concentrate, which relates to the field of production and preparation of pesticides. A formulation of the amitraz emulsifiable concentrate comprises the following components: 202 kilograms per ton of raw amitraz, 120 kilograms per ton of emulsifying agent, 100 kilograms per ton of DMF, and 10 kilograms per ton of potentiating agent; and proper amount of dimethyl benzene is complemented. The amitraz emulsifiable concentrate is amitraz series broad spectrum acaricide, is mainly used for inhibiting the activity of monoamine oxidase, has the functions of contact toxicity, feeding resistance and repelling, and also has certain functions of internal absorption and fumigating. The amitraz emulsifiable concentrate is suitable for mites of various crops, and has better prevention effect on homoptera pests. The amitraz emulsifiable concentrate has a good pesticidal effect, avoids uninterrupted alternated use of pesticides, does not pollute the environment, and improves the quality and sanitation quality safety level of fruits.

The invention belongs to the technical field of biochemical analysis and particularly relates to a hyperpolarization <129>Xe switch type magnetic resonance molecular probe for identifying diamine oxidase. The hyperpolarization <129>Xe switch type magnetic resonance molecular probe comprises cucurbit[6]uril and putrescine hydrochloride. The principle of the molecular probe is characterized in thata substrate (putrescine hydrochloride) with high cucurbit[6]uril binding force is converted by diamine oxidase into a product (1-pyrroline) which does not combine with cucurbit[6]uril; before enzymatic reaction, a <129>Xe hyperpolarization chemical exchange saturation transfer signal in the cucurbit[6]uril is inhibited by strong cucurbit[6]uril conjugate until the enzymatic reaction oxidizes the substrate into the product; due to the fact that the binding constant of putrescine hydrochloride@cucurbit[6]uril is several magnitude orders larger than that of <129>Xe@cucurbit[6]uril and (1-pyrroline)@cucurbit[6]uril cannot combine, the <129>Xe hyperpolarization chemical exchange saturation transfer signal in the cucurbit[6]uril appears after the enzymatic reaction through enhanced <129>Xe cucurbit[6]uril interaction, and diamine oxidase identification is achieved through the <129>Xe hyperpolarization chemical exchange saturation transfer signal in the cucurbit[6]uril.

The invention relates to a method for rapidly detecting the diamine biogenic amine by using the inorganic hybrid nanoflower enzyme. The method comprises the following steps of: 1) adding metal ions into a diamine oxidase solution to prepare an inorganic hybrid nanoflower enzyme dispersion liquid; 2) mixing a diamine biogenic amine solution with the inorganic hybrid nanoflower enzyme dispersion liquid, and carrying out a reaction at 45-55 DEG C for 10-30 minutes to obtain a reaction liquid; 3) adding a horse radish peroxidase solution and a TMB color developing solution, and adding diluted hydrochloric acid to terminate the reaction after color development to obtain a sample; and 4) putting the sample into a spectrophotometer to detect an absorbance value so as to obtain the concentration of the diamine biogenic amine. Compared with the prior art, the method has the following advantages of simple operation process and low professional requirements; experimental results can be directly judged by naked eyes, which enables simplicity and clearness; and results can be obtained quickly within about 25 min in a whole process, and so that the method is suitable for on-site detection; and the enzyme activity of the inorganic hybrid nanoflower enzyme is increased by 85.27% compared with the enzyme activity of common liquid enzymes.

The present invention refers to diamine oxidase for use in the treatment or prevention of attention deficit hyperactivity disorder (ADHD).

The invention relates to a recombinant engineering strain of anammox bacterium diamine oxidase. The recombinant engineering strain has a base sequence represented by SEQ ID No. 1. According to the engineering strain disclosed by the invention, the expression time of the anammox bacterium diamine oxidase is shortened, the expression level and functional activity of the gene are greatly improved, and the application of the anammox bacterium diamine oxidase to an anaerobic ammonia oxidation process is realized. The recombinant engineering strain of the anammox bacterium diamine oxidase is fermented and then is applied to the treatment of wastewater with a low carbon-nitrogen ratio.

The present invention provides pharmaceutical compositions comprising droxidopa alone, or in combination with one or more further active ingredients, for the treatment of conditions, such as mood disorders, sleep disorders, or attention deficit disorders. In certain embodiments, the compositions useful in the methods of the invention comprise droxidopa and a compound selected from the group consisting of DOPA decarboxylase inhibiting compounds, catechol-O-methyltransferase inhibiting compounds, cholinesterase inhibiting compounds, monoamine oxidase inhibiting compounds, norepinephrine reuptake inhibiting compounds, selective serotonin reuptake inhibiting compounds, tricyclic antidepressant compounds, serotonin norepinephrine reuptake inhibiting compounds, norepinephrine dopamine reuptake inhibiting compound, noradrenergic and specific serotonergic antidepressants, and combinations thereof. The inventive compositions are particularly useful in the treatment of depression, narcolepsy, insomnia, and Attention Deficit / Hyperactivity Disorder (AD / HD).

The present invention relates to functional foods comprising DAO and their use for the prevention or treatment of diseases or pathological conditions associated with high levels of histamine in blood, in particular for the prevention or treatment of migraine, chronic fatigue, fibromyalgia, spondylitis and pain caused by muscle contractures.

The invention provides a kit for detecting the barrier function of the intestinal tract. The kit comprises a reaction device, one or more colorimetric solutions and one or more dilution solutions; the reaction device comprises a blood plasma separator and a reaction substrate, and the reaction substrate includes a blank hole, a diamine oxidase reaction hole, a D-lactic acid reaction hole and a bacterial endotoxin reaction hole; a blood plasma separating film is arranged in each of the holes of the blood plasma separator; and a blank pad is arranged in the blank hole.

The invention relates to the technical field of agriculture, and discloses a method for increasing content of GABA (gamma-aminobutyric acid) in brown rice. The method comprises the following steps of increasing the content of polyamine substance of related metabolites in a rice polyamine metabolic pathway, so as to reach the purpose of increasing the content of GABA in the brown rice; enhancing the expression of related genes in the polyamine metabolic pathway in the brown rice seeds brown rice, such as arginine decarboxylase genes, ornithine decarboxylase genes, polyamine oxidase genes or diamine oxidase genes; or performing adverse treatment on the rice, and inducing to increase the content of related polyamine metabolites in the brown rice. The method has the advantages that the content of GABA in the brown rice is greatly increased, the operation is convenient, and the effect is obvious; the method can be applied to common normal-embryo brown rice, and huge-embryo brown rice.