Method for rapidly detecting diamine biogenic amine by using inorganic hybrid nanoflower enzyme

Abstract

The invention relates to a method for rapidly detecting the diamine biogenic amine by using the inorganic hybrid nanoflower enzyme. The method comprises the following steps of: 1) adding metal ions into a diamine oxidase solution to prepare an inorganic hybrid nanoflower enzyme dispersion liquid; 2) mixing a diamine biogenic amine solution with the inorganic hybrid nanoflower enzyme dispersion liquid, and carrying out a reaction at 45-55 DEG C for 10-30 minutes to obtain a reaction liquid; 3) adding a horse radish peroxidase solution and a TMB color developing solution, and adding diluted hydrochloric acid to terminate the reaction after color development to obtain a sample; and 4) putting the sample into a spectrophotometer to detect an absorbance value so as to obtain the concentration of the diamine biogenic amine. Compared with the prior art, the method has the following advantages of simple operation process and low professional requirements; experimental results can be directly judged by naked eyes, which enables simplicity and clearness; and results can be obtained quickly within about 25 min in a whole process, and so that the method is suitable for on-site detection; and the enzyme activity of the inorganic hybrid nanoflower enzyme is increased by 85.27% compared with the enzyme activity of common liquid enzymes.

Description

technical field [0001] The invention belongs to the technical field of biogenic amine detection, and relates to a method for rapidly detecting diamine biogenic amines by using an inorganic hybrid nanoflower enzyme. Background technique [0002] Biogenic amines are a class of non-volatile and biologically active low-molecular nitrogen-containing organic bases. According to the number of amino groups, they can be divided into monoamines and diamines. Among them, diamine biogenic amines mainly include histamine and putrescine. Biogenic amines are mainly formed by free amino acids under the catalysis of decarboxylase in bacteria, so there are often a certain amount of biogenic amines in foods rich in amino acids, such as dairy products, fermented wine, pickled aquatic products and meat products, etc. . [0003] Low concentrations of biogenic amines are normal active ingredients in organisms and have important physiological functions in biological cells, but ingestion of high co...

AI Technical Summary

Problems solved by technology

Putrescine mostly exists in spoiled food, and the increase of putrescine will not only have a negative impact on the flavor, but also pose a potential threat to food safety and human health

Eating foods high in putrescine can lead to serious toxicological consequences

[0004] In recent years, many analytical methods have been developed to detect such biogenic amines. The most widely used methods are high performance liquid chromatography, capillary gas chromatography, capillary zone electrophoresis, ion exchange chromatography, etc. The advantages of these analytical methods are The separation effect is good and the detection limit is low, but the disadvantages are also obvious, such as expensive instruments, professional operation, complicated steps, and the need for a large amount of organic solvents, etc.

Images