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Amperometric biosensor for histamine determination

A biosensor, histamine technology, applied in the field of seafood and fish tissue, can solve the problems of wasting time, complicated and expensive instruments, toxic reagents, etc.

Inactive Publication Date: 2010-05-19
UNIVERSITI PUTRA MALAYSIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require complex and expensive instruments, toxic reagents, waste time and cannot be used for in situ analysis due to their complicated sample handling, requiring experienced people to test

Method used

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  • Amperometric biosensor for histamine determination
  • Amperometric biosensor for histamine determination
  • Amperometric biosensor for histamine determination

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Embodiment Construction

[0019] The invention provides an ampere-type biosensor for detecting the value of histamine in a sample, wherein immobilized diamine oxidase is used as a bioreceptor. In a first embodiment of the present invention, a biosensor includes a working electrode, a counter electrode and a reference electrode. Preferably, the biosensor comprises a screen printed carbon paste working electrode, a platinum rod counter electrode and a silver / silver chloride (Ag / AgCl) reference electrode.

[0020] Diamine oxidase was immobilized on the surface of the screen-printed working electrode by poly-2-hydroxyethyl methacrylate (photo-HEMA). Before fixation, the enzyme was dissolved in 0.1 M phosphate buffer at a pH ranging from 6.4 to 8.4, with an optimal pH of 7.4. Light HEMA was prepared as reported by Low and coworkers (2005). Mix poly-2-hydroxyethyl methacrylate and photoinitiator 2'-dimethylphenylacetophenone (DMPP). The mixture was shaken until DMPP was dissolved. The evenly distributed ...

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Abstract

There is provided by this invention a simple and rapid amperometric biosensor for determining the level of histamine in seafood or fish. The biosensor combines the technology of screen-printing with immobilized diamine oxidase as the bioreceptor. IQ one embodiment of the present invention, the biosensor incorporates potassium hexacyanoferrate (III) as a mediator.

Description

technical field [0001] The invention relates to an amperometric biosensor for the detection of histamine, especially seafood and fish tissue. Background technique [0002] For many countries around the world, seafood and fish products are important for their nutritional value, as well as for international trade and foreign exchange earnings. Unlike other animal products, the quality of seafood and fish products is more difficult to control due to the variation in species, sex, age, habitat and autolytic enzyme activity (Venugopal, 2002). Histamine values ​​have been considered as indicators of rapid seafood and fish spoilage (Male et al., 1996; Tombelli and Mascini, 1998; Patange et al., 2005). During product storage, when bacterial spoilage begins, histamine accumulation can be seen (Male et al. 1996) without altering the normal appearance and smell of seafood and fish (Lehane and Olley, 2000). Therefore, the food industry is in great need of simple and rapid techniques f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/28G01N27/30C12Q1/26
CPCG01N2333/90633G01N33/12G01N27/3277C12Q1/26
Inventor 法提马·阿布·巴卡阿布·巴卡·沙尼华文·华基兰程美乔李毓亨安鲁阿·阿麦罗斯令·穆特法
Owner UNIVERSITI PUTRA MALAYSIA
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