The invention discloses a microbial
fermentation method for synthesizing
triterpene compounds, namely a distillers
yeast recombination strain SY2 containing cucurbitane dienol synthetase SgCbQ, P450 CYP87D18 and
cytochrome reductase CPR, in the
metabolism route of mogrol, which takes dextrose as a substrate to synthesize three new products including 11-
oxygen-cucurbitane dienol, 11-hydroxy-cucurbitane dienol and 11-
oxygen-24, 25-
epoxy-cucurbitane dienol. The invention further discloses a
metabolism route for synthesizing mogrol through cucurbitane dienol, namely the cucurbitane dienol is catalyzed through P450 CYP87D18 and synthesized into 11-
oxygen-cucurbitane dienol, 11-oxygen-cucurbitane dienol is catalyzed through
enzyme and synthesized into 11-hydroxy-cucurbitane dienol and 11-oxygen-24, 25-
epoxy-cucurbitane dienol, and 11-hydroxy-cucurbitane dienol and 11-oxygen-24, 25-
epoxy-cucurbitane dienol can be synthesized into the mogrol through other P450 or
epoxide hydrolase. The invention further discloses a novel thought for obtaining more
glycosylation products by catalyzing new products through
glycosyl transferase. The method has significance for determining the synthesis
route of mogrol and synthesizing relevant intermediate metabolites and
glycosylation products through the microbial
fermentation method.