The invention discloses a tissue culturing method of
begonia semperflorens. The method comprises the following steps: (1) sterilization: selecting an explant of the
begonia semperflorens; washing theexplant of the
begonia semperflorens with
tap water, then soaking the explant with
alcohol, then cleaning the explant with
sterile water, and then sterilizing the explant with
sodium hypochlorite, washing the explant with
sterile water for the second time, and
cutting and inoculating the explant of the begonia semperflorens; (2) soaking by
nutrient solution, preparing a 6-BA
nutrient solution of 1 / 2MS+9.0mg / L, soaking the explant with the 6-BA
nutrient solution for 1 hour at 25 DEG C; (3) tissue culturing of leaf blades: putting the explant soaked with the
nutrient solution in the step (2) into culture media having different hormones and proportions, and sequentially inducing the explant to form wound
callus, inducing the wound
callus to generate buds, inducing the buds to carry out proliferation and differentiation, and inducing the buds to carry out rooting so as to obtain adult seedlings; (4)
seedling exercising and
transplantation: putting the adult seedlings obtained in the step (3) into a culture
bottle, opening a lid of the culture
bottle and carrying out
seedling exercising for 2 days in a room, taking out the adult seedlings and cleaning a base part culture medium, and
transplanting into a matrix in which the proportion of
perlite to humic matter is 1 to 2.