Tissue culturing method of begonia semperflorens

Abstract

The invention discloses a tissue culturing method of begonia semperflorens. The method comprises the following steps: (1) sterilization: selecting an explant of the begonia semperflorens; washing theexplant of the begonia semperflorens with tap water, then soaking the explant with alcohol, then cleaning the explant with sterile water, and then sterilizing the explant with sodium hypochlorite, washing the explant with sterile water for the second time, and cutting and inoculating the explant of the begonia semperflorens; (2) soaking by nutrient solution, preparing a 6-BA nutrient solution of 1 / 2MS+9.0mg / L, soaking the explant with the 6-BA nutrient solution for 1 hour at 25 DEG C; (3) tissue culturing of leaf blades: putting the explant soaked with the nutrient solution in the step (2) into culture media having different hormones and proportions, and sequentially inducing the explant to form wound callus, inducing the wound callus to generate buds, inducing the buds to carry out proliferation and differentiation, and inducing the buds to carry out rooting so as to obtain adult seedlings; (4) seedling exercising and transplantation: putting the adult seedlings obtained in the step (3) into a culture bottle, opening a lid of the culture bottle and carrying out seedling exercising for 2 days in a room, taking out the adult seedlings and cleaning a base part culture medium, and transplanting into a matrix in which the proportion of perlite to humic matter is 1 to 2.

Description

technical field [0001] The invention relates to a propagation method of begonias, in particular to a tissue culture method of begonias in four seasons. Background technique [0002] Four Seasons Begonia, also known as Begonia and Melon Seed Begonia, is a perennial fleshy evergreen herbaceous flower belonging to Begoniaceae and Begonia. Stems erect, 15-30cm high; The leaves are mostly ovate, about 5-8cm in length and 1-3cm in width. The surface of the fleshy leaves is smooth and the lower part is slightly inclined. The edge of the leaves has coarse serrations, and can be divided into green-leaf strains and red-leaf strains according to leaf color. Four Seasons Begonia is a green-leaf strain, with shiny green leaves, reddish edges, and red flowers. They are clustered on the peduncle and appear luxuriant, and have a long flowering period. Under suitable temperature, humidity and light, they can bloom almost all year round. . Therefore, it is an excellent choice for ground ...

AI Technical Summary

Problems solved by technology

[0003] The seeds of perennial begonias are very small, so it is not easy to collect and store the seeds

However, because the stems of Begonia four seasons are fleshy and are susceptible to contamination by fungi and viruses, the stems are extremely easy to rot during cutting propagation.

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