45 results about "Flower induction" patented technology

A method for regulating butterfly orchid florescence is characterized by conducting flower induction 20 days earlier than the expected florescence; illuminating the flower for eight hours every day and covering the flower in dark for the rest time; mixing the inner flowerpot substrate of turfy soil with perlite at a volume ratio of 5:1 to 6:1; drenching the whole substrate with clear water with pH value between 6.0 to 6.8; controlling the greenhouse temperature between 8 DEG C to 12 DEG C, the humidity between 70% to 80% in the daytime and 75% to 85% during the nighttime, illumination between 8000 Lux to 10000 Lux; seven days after the first watering, pouring clear water with pH value between 6.0 to 6.8 when the EV value is higher than 0.4, and pouring aqueous solution containing 0.3-0.4g Peters Professional 20-10-20, 0.05-0.06g Peters Professional 15-20-25 and 0.16-0.18g Mg SO4 in every liter when the EV value is lower than 0.4 , and then pouring water solution containing KNO 3 0.8-0.9g / L for one time.

The invention relates to an air pineapple flowering time regulation method. According to the method, an air pineapple is induced to flower by the aid of plant growth regulator ethephon. The method includes the steps: (1) forcing flowers of plants; (2) performing floral induction; and (3) accelerating flower bud development. By the aid of the air pineapple flowering time regulation method, the flowering rate of the air pineapple can reach more than 90%, ornamental value and commercial value of the air pineapple are greatly improved, agent cost is low, and an operating method is simple and easy.

The invention discloses a method for establishing a lotus regeneration system. The method comprises the following steps: breaking shells of sterilized Guangchang white lotus seeds by using pruning shears; taking out mature lotus nuts (embryos) as explants to culture aseptic seedlings; cutting 2-3mm of stem tips and folded tender leaves (with short petiole) as explants for inducing callus from the aseptic seedlings after 2 months; inducing and differentiating the callus to develop buds after 2 months; after 1 month, cutting off the black callus at the bottom, and proliferating the differentiated seedlings; after 3 months, inducing the differentiated seedlings to develop roots; and strengthening, domesticating and transplanting the rooted tissue culture seedlings after 1 month. By adopting the method, technical basis is provided for transgenic technology of lotus in China, and the inductive callus rate of lotus is increased and the callus is large and stable in size compared with a conventional technique; the propagation coefficients of adventitious buds are increased; as tender leaves of aseptic seedlings of lotus are innovatively taken as explants for inductive callus and are successfully differentiated to obtain seedlings, the material selection of lotus inductive callus is widened, and material guarantee is provided.

The present invention relates to an artificial inductive flowering asexual breeding method of bulbil konjak. Said method includes the following steps: making the bulbil konjak bulb undergo the process of flowering induction, inhibiting germination of lateral bud on the bulb and making the root tip grown out on the bulb be perished, promoting differentiation of top bud of konjak bulb to form flower bud, after the bulb is transplanted, artificially inhibiting flowered leafing plant growth, making flower bract can be matured and seeded so as to obtain lots of seeds capable of reproducing konjak.

The invention discloses a method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulators. The method comprises the following steps: culturing Dendrobium nobile for 45 days in an alpine greenhouse, keeping day and night temperatures in the alpine greenhouse at 18-31 DEG C and 7-18 DEG C, respectively, and spraying plant growth regulator solution to Dendrobium nobile stems every 10 days during the culture; after alpine greenhouse cultivation, transferring Dendrobium nobile to a greenhouse in a plain, keeping the day and night temperaturesat 25 DEG C and 20 DEG C, respectively, and culturing for 60-70 days till Dendrobium nobile plants flower. The method of the invention can promote flower bud induction and normal flowering for Dendrobium nobile, significantly increase normal flowering rate of plants, normal flowering rate of nodes and average number of flowers per node, thus greatly improving the viewing quality and commercial value of Dendrobium nobile. Compared with alpine flower induction method in prior arts, the method can achieve a better flower induction effect in the aspects of normal flowering rate, flower number perplant and the like, flower quality has been markedly improved, and no harm is caused by the use of plant growth regulators.

A plant comprising a modified FT polynucleotide expressing a modified polypeptide exhibits altered flowering time, floral numbers and / or increased seed production. Different mutant sequences conferring different phenotypes are disclosed.

The invention belongs to the field of banana callus irradiation mutation breeding, and particularly relates to a screening method for cold resistant banana germplasm. According to the method, the cold resistant banana germplasm can be quickly and efficiently screened. The screening method for the cold resistant banana germplasm comprises the following steps of: the irradiation treatment of the immature male flower callus of bananas, the low-temperature screening of the callus, the low-temperature screening of a differentiated bud, the low-temperature screening of a banana seedling and banana cold resistant rooting seedling acclimation and transplantation. According to the method, because the low-temperature screening technology is adopted for three times, especially, the low-temperature screening technology is adopted during the period of the male flower induction callus, so that the screening efficiency of the cold resistant mutation germplasm is greatly improved, and the period for culturing a cold resistant banana variety is shortened.

The invention discloses a flower formation regulator gene LcFLC obtained from litchi chinensis by separation and application thereof, belongs to the field of biological genes. The invention particularly discloses the nucleotide sequence, cloning method and subcellular localization of the gene LcFLC; due to spatio-temporal expression features at different tissue parts of litchi chinensis, the gene LcFLC has the function of retarding the flowering time of plants. According to the gene LcFLC for retarding the flowering time of plants and the application thereof, provided by the invention, the preliminary knowledge about the floral induction mechanism of litchi chinensis is gained for the first time from the perspective of molecular biology, a foundation is laid for regulating the flowering time by overexpression or expression inhibition of target genes in litchi chinensis, and the gene LcFLC has a very important significance to breeding of new litchi chinensis species by utilizing genetic engineering.

The invention discloses a dendrobium nobile florigen DnFT gene and a cloning method and application thereof. The nucleotide sequence of the dendrobium nobile florigen DnFT gene is shown as SEQIDNo1, and the amino acid sequence of the coded polypeptide is shown as SEQIDNo2. The dendrobium nobile florigen DnFT gene is tested to be capable of promoting flowering of plants, providing fundamental basis for the DnFT gene in a floral induction process of dendrobium nobile, providing theoretical basis for building of flower induction path networks of the dendrobium nobile, and providing new gene resources for using of the DnFT gene to improve the dendrobium nobile or other flowers and plants to obtain new flowers and plants with a shortened growth cycle in the future.

The invention discloses a water culture method of ornamental and edible pineapples, belonging to the technical field of farming. The water culture method comprises the following steps: selecting plants of good pineapple varieties; trimming leaves and sterilizing, putting the processed plants into field planting basins filled with culture liquid and carrying out water culture; spraying nutrient growth liquid onto the plants every 5-7 days; after leaves of the pineapple plants grow to 35cm or more and the quantity of the leaves reaches 20-30, carrying out plant filling and flower induction by utilizing a calcium carbide water solution; spraying a microelement foliar fertilizer when the plants have red buds; uniformly spraying surfaces of the pineapples by utilizing a potassium dihydrogen phosphate solution from a small fruit period to an expanding period of the pineapples; and harvesting when the fruits of the pineapples grow to become golden yellow color or remaining the fruits on trees for 5-15 days and picking when the fruits become soft and the color is changed. According to the water culture method disclosed by the invention, the eating and planting requirements of the pineapples are met; and meanwhile, domestic ornamental planting of the pineapples can be realized and the limitation of planting regions is broken.

The invention discloses a longan gene DlGRAS34, a protein and an application of the longan gene DlGRAS34 and the protein in regulating and controlling flowering of plants. A cDNA nucleotide sequence of the longan gene DlGRAS34 is shown as SEQ ID No.1. The full length of an open reading frame of the longan gene DlGRAS34 is 1578bp, 525 amino acids are encoded, and the longan gene DlGRAS34 has a typical GRAS structural domain and a DELLA structure and belongs to GRAS protein. A qRT-PCR result shows that the gene has tissue expression specificity, has the highest relative expression quantity in pericarp, and has the second relative expression quantity in stem, leaf and flower organs; expression is down-regulated in the early flowering induction stage of 'Sijimi' longan, and expression is up-regulated in the later flowering stage of 'Shixia' longan. A transient expression result of the arabidopsis protoplast shows that a fluorescence signal is mainly concentrated on a cell nucleus. A transgenic arabidopsis thaliana result shows that a DlGRAS34-overexpressed transgenic plant shows a late flowering phenomenon that a wild type plant blooms in about 27 days, and a transgenic line blooms in 32-35 days; and meanwhile, rosette leaves become large, and plants become short. The results show that as a typical transcription factor, the protein coded by the DlGRAS34 is located in a cell nucleus, plant flowering is negatively regulated, and the overexpression of the DlGRAS34 gene can significantly inhibit plant flowering.

The invention discloses a taro sexual reproduction breeding method which comprises the following steps of: 1, taro seed permanent planting; 2, flowering induction, namely performing flowering induction by spraying a chemical on taro leaves, wherein the composition of the chemical comprise 200-500mg / L gibberellin; 3, pollination; 4, seed collection; 5, seedling raising, namely configuring cover soil, then performing disinfection treatment on the cover soil, sprouting selected seeds, performing seedling hardening, transplanting and performing seedling-stage management; 6, breeding, namely primarily selecting excellent individual plants with vigorous growth potential and less diseases, marking and numbering for observation, reselecting according to observation record information and comprehensive analysis results, performing primary reproduction and strain comparative test on the selected individual plants, and breeding the excellent individual plants selected through the strain comparative test and local adaptability experimental planting, so as to obtain the excellent taro seedlings. According to the method disclosed by the invention, exogenous hormone is utilized to induce the taro variety to flower, and a sexual reproduction technology is utilized to breed a new taro variety, so that conditions are provided for researching taro genetic regularity and breeding excellent new varieties through hybridism.

The invention provides a longevity flower induction method. The method includes utilizing 1000-time 'flower increasing No 1' liquid for irrigating per week, stopping fertilization when the 9th to 11th leaf grows, topping, and applying 600 to 2000ppm chlormequat chloride water solution and 600 to 2000 time cytokinin liquid per week. The growth and development of longevity flower are promoted positively, the number of the longevity flower is increased particularly, and the viewing value of the longevity flower is increased.

The invention discloses a spring peach blossom flowering phase regulating method. In 30-50 days before flowering at a designated time, flowering induction processing is performed, a chemical agent is sprayed on each whole tree during flowering induction, so that spring peach blossoms flower at the designated time, wherein the chemical agent is any one of lime nitrogen, thiourea, hydrogen cyanamide and a leaf fertilizer. The chemical method has a flowering induction effect. In general, the effects of the lime nitrogen and the leaf fertilizer adopted in the chemical method are good, a flowering phase can be advanced for 7-8 days, the flowering rate is high, ornamental characteristics are also well improved, and the spring peach blossom flowering phase regulating method can be applied to solving of the actual spring peach blossom flowering phase regulating and controlling problem. By combining with test results in previous two years, the thiourea and the hydrogen cyanamide also have a good flowering induction effect, but the effect is not stable.

The invention discloses a method for inducing plants to bloom early by a populus tomentosa sepallata (PtSEP) gene of poplar. In the method, the total length of complementary deoxyribonucleic acid (cDNA) of the PtSEP gene, which is the target gene, of the poplar to construct an ultra-expression vector of the PtSEP gene, and the ultra-expression vector is transferred into a plant to advance the blossom time of the plant such as tobacco and the like. The early flowering induction of the plants and particularly flowers is performed by utilizing the transgenic technology, the period is short and the effectiveness is quick.

The invention relates to the field of plant cultivation, in particular to a flowering induction method for dendrobium nobile seedlings. The method adopts the combination of hormone induction, temperature and nutritional regulation, the problems are solved that the flowering period of the dendrobium nobile seedlings is long, and flowering is not uniform. By adopting suitable hormone concentration,spraying frequency, spraying time and reasonable low-temperature treatment temperature and time and by utilizing the C / N ratio and beneficial components of liquid-state carbon-contained compound fertilizer, the combination of nutrition regulation is conducted to induct flowering of the dendrobium nobile seedlings, multiple pseudo bulbs of dendrobium nobile can be inducted to flower, the initial flowering period is short, the quantity of flowers is large, flowering is regular, ornamental value is high, and the new method is provided for cultivation of competitive product dendrobium nobile.

The invention provides a method for in-vitro flowering of dendrobium officinale. The method comprises: propagation culture, rooting culture, flowering culture, and routine culture till flowering. According to the method, the explant disinfection efficiency can reach as high as 90%; the propagation multiple can reach 4; the aseptic seedling rooting rate can reach 100%; the normal flower induction rate can reach a highest value of 84% and a lowest value of 25%; and each plant blossoms with unequal numbers of 1-3 of flowers. According to the method for test-tube in-vitro flowering of dendrobium officinale, the problems that the flowering period of dendrobium officinale in natural and culture conditions is relatively long, and dendrobium officinale easily infects diseases and pest are greatly solved. Dendrobium officinale seedlings can be quickly provided, and the culture cycle is shortened, so that market requirements are met.

The present invention relates to an artificial inductive flowering asexual breeding method of bulbil konjak. Said method includes the following steps: making the bulbil konjak bulb undergo the process of flowering induction, inhibiting germination of lateral bud on the bulb and making the root tip grown out on the bulb be perished, promoting differentiation of top bud of konjak bulb to form flower bud, after the bulb is transplanted, artificially inhibiting flowered leafing plant growth, making flower bract can be matured and seeded so as to obtain lots of seeds capable of reproducing konjak.

The invention belongs to the field of flower plantation technology and provides a plantation method for raising survival rate of transplanting ginkgo. The method of the invention comprises the following steps: (1) in the plantation area of ginkgo trees, a soil sample is acquired in mid-September each year after harvesting of ginkgo, and active content of major elements, medium elements and trace elements in the soil sample are measured; (2) according to the age of the ginkgo trees, the ginkgo trees are fertilized, and an efficient composite fertilizer with the total nutrient content of N+P2O5+K2O being greater than or equal to 50% is adopted; (3) in May, August and November each year, the ginkgo trees are respectively fertilized with a fertilizer for accelerating germination and flower induction, a fertilizer for fructification and a fruit-withering fertilizer; the fertilizing amount is determined according to diameter of the crown: Y =1 / 10 pi r2 (wherein Y represents the fertilizing amount, unit is kilogram, r represents radius of the crown, unit is meter), and the fertilization mode of the fertilizer for accelerating germination and flower induction is surface sprinkling; and the fertilization mode of the fertilizer for fructification and the fruit-withering fertilizer is root fertilization. The survival rate of transplanting is high and reaches 97%, and the trees grow well.

The invention discloses a method for improving blooming quality of chaenomeles x superba, and relates to the technical field of regulation and control of plant blooming. The method comprises the following steps: (1) choosing a kinetin with an appropriate concentration and spraying the kinetin to leaf surfaces of chaenomeles x superba seedling trees in a flower-bud differentiation phase; (2) timely performing weeding and controlling rust diseases for the seedling trees of chaenomeles x superba in a growth phase, and irrigating the seedling trees with anti-freezing water in a flower induction phase, wherein it is better to control the water to be frozen in night and thawed in daylight; and (3) during the flower induction phase of the chaenomeles x superba, the potted chaenomeles x superba seedling trees are put in an artificial climate room for temperature-increased culture. The method can help to increase the blooming rate, the total blooming quantity and the flower forming quantity on inflorescences of the chaenomeles x superba, and effectively prevent the phenomenon that flower buds do not bloom. Also, the method is capable of reducing the color difference between the inner loop and the outer loop on petals and improving the flower color saturation degree, solving the flower color deterioration problem, and has good promotion and application values by improving the blooming quality of the chaenomeles x superba and improving the utilization rate of the chaenomeles x superba flower resources.

The invention discloses an establishment method of a rosachinensis micro-propagation system. The method comprises the following five steps of sterilizing an explant of container rosachinensis, carrying out bud induction culture, carrying out bud propagation culture, carrying out flower induction culture, and carrying out rooting culture, so that the container rosachinensis is successfully cultured in a culture bottle. A culture medium used in a bud induction culture process is MS+TDZ 1.0mg / L + NAA 0.1mg / L +agar 7.2g / L + saccharose 30g / L, and a culture used in a bud propagation culture process is MS+TDZ 1.0mg / L + NAA 0.05mg / L +agar 7.2g / L + saccharose 30g / L. The rosachinensis cultured through the method is higher in flower induction ratio and rooting rate and high in survival rate, has a great economic value, and lays a foundation for follow-up molecular biology study.

The invention discloses a flowering induction method for sweet potatoes. The flowering induction method for the sweet potatoes comprises the following steps: 1) when the ground temperature is stable at 15 DEG C or higher, sweet potato seedlings are planted in protected land with a flat ridge double-plant planting method; 2) when the sweet potato seedlings turn green and apical leaves stretch, thesweet potato seedlings are subjected to short-day treatment until pollination of the sweet potatoes is finished; 3) when the sweet potato seedlings grow to about 50 cm long, apexes of the sweet potatoseedlings are treated with gibberellin, and flowering induction of the sweet potatoes is completed. With adoption of the method, flowering time of the sweet potatoes can be shortened, and flowering quantity is increased, so that the method is of great significance for increasing the yield of the sweet potatoes.

The invention belongs to the field of banana callus irradiation mutation breeding, and particularly relates to a screening method for cold resistant banana germplasm. According to the method, the cold resistant banana germplasm can be quickly and efficiently screened. The screening method for the cold resistant banana germplasm comprises the following steps of: the irradiation treatment of the immature male flower callus of bananas, the low-temperature screening of the callus, the low-temperature screening of a differentiated bud, the low-temperature screening of a banana seedling and banana cold resistant rooting seedling acclimation and transplantation. According to the method, because the low-temperature screening technology is adopted for three times, especially, the low-temperature screening technology is adopted during the period of the male flower induction callus, so that the screening efficiency of the cold resistant mutation germplasm is greatly improved, and the period for culturing a cold resistant banana variety is shortened.

The invention discloses a flowering regulation gene LliMADS11 separated from an endangered mangrove plant lumnitzera littorea and an application method of the flowering regulation gene LliMADS11 in thermomorphogenesis, wherein the nucleotide sequence is shown as SEQ ID NO.1, and the amino acid sequence coded by the LliMADS11 gene is shown as SEQ ID NO.2. The LliMADS11 gene contains a structural domain of an MADS family. A recombinant plasmid is obtained after the LliMADS11 gene is connected with a pBinGlyRed vector, and agrobacterium is transformed by a freeze-thaw method. After the arabidopsis thaliana is transformed by a pollen tube channel method, the rosette leaves of transgenic arabidopsis thaliana is 11 leaves fewer in average than the rosette leaves of wild arabidopsis thaliana, andthe flowering period of the transgenic arabidopsis thaliana is obviously put forward. According to the invention, the flowering induction mechanism of the lumnitzera littorea is preliminarily known from the viewpoint of molecular biology for the first time, so that a foundation is laid for regulating the flowering time through over-expression of the target gene in lumnitzera littorea or expression inhibition of the target gene in lumnitzera littorea, and the application method has important significance for clarifying the danger of lumnitzera littorea from the molecular level.

The invention provides a method for in-vitro flowering of dendrobium officinale. The method comprises: propagation culture, rooting culture, flowering culture, and routine culture till flowering. According to the method, the explant disinfection efficiency can reach as high as 90%; the propagation multiple can reach 4; the aseptic seedling rooting rate can reach 100%; the normal flower induction rate can reach a highest value of 84% and a lowest value of 25%; and each plant blossoms with unequal numbers of 1-3 of flowers. According to the method for test-tube in-vitro flowering of dendrobium officinale, the problems that the flowering period of dendrobium officinale in natural and culture conditions is relatively long, and dendrobium officinale easily infects diseases and pest are greatly solved. Dendrobium officinale seedlings can be quickly provided, and the culture cycle is shortened, so that market requirements are met.

The invention discloses a LliMADS11 gene isolated from Prunus oleifera and an application method thereof. The gene has the property of promoting flowering of plants. The present invention preliminarily understands the flowering induction mechanism of Prunus vulgaris from the perspective of molecular biology for the first time, and lays the foundation for regulating the flowering time by overexpressing or inhibiting the expression of the target gene in Prunus vulgaris in the future. The endangerment of red olive plum is of great significance.

The invention provides a method for inducing flowering of caryophyllaceae plant. Specifically, the method for inducing flowering of caryophyllaceae plants provided by the invention comprises the following steps: (a) cultivating sterile bud seedlings of caryophyllaceae plant to obtain bud seedlings in the vegetative period; (b) continuously cultivating the bud seedlings in vegetative period obtained in the step (a) so as to obtain bud seedlings in the reproductive growth conversion period; (c) cultivating the bud seedlings in the reproductive growth conversion period obtained in the step (b), and rooting to obtain a flowering plant. According to the method, the sterile bud seedlings of caryophyllaceae plant is firstly taken into an initial explant, in vitro flower induction is carried out to obtain effective sterile pollen, an vitro flower induction system of caryophyllaceae plant is established, and reliable basis is established for the cultivation of haploid double haploid breeding material of caryophyllaceae.

The invention discloses a method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulators. The method comprises the following steps: culturing Dendrobium nobile for 45 days in an alpine greenhouse, keeping day and night temperatures in the alpine greenhouse at 18-31 DEG C and 7-18 DEG C, respectively, and spraying plant growth regulator solution to Dendrobium nobile stems every 10 days during the culture; after alpine greenhouse cultivation, transferring Dendrobium nobile to a greenhouse in a plain, keeping the day and night temperaturesat 25 DEG C and 20 DEG C, respectively, and culturing for 60-70 days till Dendrobium nobile plants flower. The method of the invention can promote flower bud induction and normal flowering for Dendrobium nobile, significantly increase normal flowering rate of plants, normal flowering rate of nodes and average number of flowers per node, thus greatly improving the viewing quality and commercial value of Dendrobium nobile. Compared with alpine flower induction method in prior arts, the method can achieve a better flower induction effect in the aspects of normal flowering rate, flower number perplant and the like, flower quality has been markedly improved, and no harm is caused by the use of plant growth regulators.

The invention provides a longan flowering regulation gene DlERF23 , its cDNA nucleotide sequence is shown in SEQ ID No.1. the longan DlERF23 The full length of the open reading frame of the gene is 552bp, encoding 183 amino acids, which has a typical AP2 / ERF domain and zinc finger structure, and belongs to the ERF protein. The results of qRT-PCR showed that the gene had tissue-specific expression, and the relative expression level was higher in pericarp, followed by stem, leaf and flower organs; the expression was down-regulated in the early flowering induction period of 'Sijimi' longan, while in the 'Sijimi' longan. The expression of Shixia'longan was up-regulated in the later stage of flowering. The results of transient expression in Arabidopsis protoplasts showed that the fluorescent signal was mainly concentrated in the nucleus. Transgenic Arabidopsis results showed that overexpression DlERF23 The transgenic plants showed early flowering: the wild-type plants flowered in about 26 days, while the transgenic lines flowered in 18‑20 days; the number of rosette leaves also increased. As a typical transcription factor, DlERF23 The encoded protein is located in the nucleus and positively regulates plant flowering, DlERF23 Gene overexpression significantly boosts plant flowering.