A screening method for cold-resistant banana germplasm

Abstract

The invention belongs to the field of banana callus irradiation mutation breeding, and particularly relates to a screening method for cold resistant banana germplasm. According to the method, the cold resistant banana germplasm can be quickly and efficiently screened. The screening method for the cold resistant banana germplasm comprises the following steps of: the irradiation treatment of the immature male flower callus of bananas, the low-temperature screening of the callus, the low-temperature screening of a differentiated bud, the low-temperature screening of a banana seedling and banana cold resistant rooting seedling acclimation and transplantation. According to the method, because the low-temperature screening technology is adopted for three times, especially, the low-temperature screening technology is adopted during the period of the male flower induction callus, so that the screening efficiency of the cold resistant mutation germplasm is greatly improved, and the period for culturing a cold resistant banana variety is shortened.

Description

technical field [0001] The invention belongs to the field of radiation mutagenic breeding of banana callus, and in particular relates to a screening method for cold-resistant banana germplasm. technical background [0002] Banana is a typical tropical and subtropical fruit tree, widely distributed in tropical and subtropical regions. my country is one of the main banana producing countries in the world, and the planting areas are distributed in Guangdong, Hainan, Guangxi, Fujian, Yunnan and Taiwan. In recent years, due to changes in climate conditions, banana planting in Guangdong, Guangxi, Yunnan and other provinces in my country has often been threatened by cold damage, resulting in large-scale production reductions and crop failures, causing serious economic losses to the majority of banana farmers. Although some preventive measures have been established for cold damage in production, the preventive effect is still unsatisfactory for more serious cold damage. Therefore, ...

AI Technical Summary

Problems solved by technology

In recent years, due to changes in climate conditions, banana planting in Guangdong, Guangxi, Yunnan and other provinces of our country is often threatened by cold damage, resulting in large-scale production reduction and failure, and causing serious economic losses to the majority of banana farmers.

Although some preventive measures have been established for cold damage in production, the preventive effect is still unsatisfactory for more serious cold damage. Therefore, breeding cold-resistant varieties of bananas is the fundamental way to solve the problem of banana cold damage.

Most of the currently cultivated bananas are triploid, parthenocarpic, and highly sterile. It is impossible to breed new cold-resistant varieties through conventional hybridization. Therefore, using 60 Co-γ-ray radiation mutagenesis bananas, cultivating banana mutants, screening new germplasm with cold-resistant mutations from mutants, and then cultivating new varieties is one of the important ways of banana cold-resistant breeding

[0003] At present, there are few domestic and foreign reports on the research on the cold-resistant breeding of banana radiation mutagenesis. In the selection of radiation mutagenesis materials, banana sucking buds, banana tissue culture buds or banana seedlings are mainly used. The main disadvantage of this method is the mutant materials that need to be processed. Many, long screening cycle and low efficiency