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Dendrobium nobile florigen DnFT gene and cloning method and application thereof

A technology of Dendrobium nobile and flowering element, applied in the field of genetic engineering, to achieve the effect of promoting flowering

Inactive Publication Date: 2012-10-03
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TFL1 has a high homology with FT, but it is a flowering inhibitor. Changing an amino acid of the TFL1 protein will turn TFL1 into a flowering activator. TFL1 can also be transported to the apical meristem through the phloem like FT protein However, the massive expression of TFL1 in the apical meristem can inhibit the expression of LFY and AP1 / AP2, thereby inhibiting Arabidopsis flowering (Hanzawa, 2005)

Method used

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  • Dendrobium nobile florigen DnFT gene and cloning method and application thereof
  • Dendrobium nobile florigen DnFT gene and cloning method and application thereof
  • Dendrobium nobile florigen DnFT gene and cloning method and application thereof

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Embodiment 1

[0042] 1. Dendrobium nobile DnF gene cloning

[0043] 1. Primer design

[0044] The constructed cDNA library has been sequenced and compared to verify that there are two FT homologous sequences. According to the obtained cDNA fragment sequence, use the biological software Primer Premier 5.0 to design two specific primers FT 3 near the 3′ end of the fragment sequence For '-RACE 1st Primer and FT 3'-RACE 2nd Primer, two specific primers Ft5raceprimer1 and Ft5raceprimer2 were designed near the 5' end of the fragment sequence (Table 1).

[0045]

[0046] 2. Dendrobium nobile DnF Gene 3′RACE and 5′RACE

[0047] (1) 3′ RACE:

[0048] The total RNA of Dendrobium nobile flower buds extracted by the CTAB method was reverse-transcribed to obtain cDNA, and the 3′ end was amplified using the nested method of the Takara 3′Full RACE Core Set Kit kit, and the primer was FT 3′-RACE 1st Primer (SEQ ID NO:3) and FT 3′-RACE 2nd Primer (SEQ ID NO:4). The PCR reaction conditions are as fo...

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Abstract

The invention discloses a dendrobium nobile florigen DnFT gene and a cloning method and application thereof. The nucleotide sequence of the dendrobium nobile florigen DnFT gene is shown as SEQIDNo1, and the amino acid sequence of the coded polypeptide is shown as SEQIDNo2. The dendrobium nobile florigen DnFT gene is tested to be capable of promoting flowering of plants, providing fundamental basis for the DnFT gene in a floral induction process of dendrobium nobile, providing theoretical basis for building of flower induction path networks of the dendrobium nobile, and providing new gene resources for using of the DnFT gene to improve the dendrobium nobile or other flowers and plants to obtain new flowers and plants with a shortened growth cycle in the future.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to anthocyanins from Dendrobium nobile DnF Genes and their cloning methods, containing DnF Gene recombinant expression vector, DnF genetically encoded polypeptides, and DnF The use of genes in promoting flowering of plant extracts. Background technique [0002] Dendrobium nobile ( Dlnobile Lindl ) is a commonly used precious Chinese medicinal material. In addition to its medicinal value, Dendrobium nobile is often used as a potted plant for indoor appreciation because of its beautiful flower shape, variety of flower colors, beautiful flower posture, pleasant fragrance, and long flowering period. It is used for table decorations and hangings. It is also a good cut flower material, very popular with people from all over the world, and has become a world-renowned ornamental flower, occupying an important position in the international flower market. However, its growth cycle is ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/10C07K14/415A01H5/00
Inventor 孙姝兰李瑞红王爱珂梁山王小菁李洪清
Owner SOUTH CHINA NORMAL UNIVERSITY
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