31 results about "Alternaria brassicae" patented technology

The present invention relates to a strain of Bacillus subtilis R31 with broad-spectrum antagonistic ability to phytopathogenic fungi, in particular to provide Bacillus subtilis R31 (Bacillus subtilis R31) bacterial strain (CCTCC NO: M 209261) and its growth to phytopathogenic fungi Inhibition, especially in the biological control of banana wilt. The plant endophytic Bacillus subtilis provided by the present invention is proved to be a new Bacillus subtilis through the obtained 16S rDNA, gyrA and gyrB gene fragments, and through BLAST search and comparison on NCBI, capable of treating various plant pathogens The fungus has good plate antagonistic activity, especially for various soil-borne vascular diseases such as Fusarium oxysporum, Rhizoctonia solani, Alternaria and other soil-borne vascular diseases, and has shown good activity in greenhouses and fields. Control effect.

The invention discloses application of a dehydroabietylamine (substituted) benzaldehyde Schiff base derivative, which is used for wood preservation. The dehydroabietylamine (substituted) benzaldehydeSchiff base derivative disclosed by the invention has an effective inhibition effect on botrytis cinerea, fusarium solani, fusarium graminearum, fusarium oxysporum, alternaria brassicae, coriolus versicolor and the like, and can be directly used for wood preservation or preparation of wood preservatives; and the raw materials are wide in sources and low in cost, and the dehydroabietylamine (substituted) benzaldehyde Schiff base derivative has the advantages of naturalness, no toxicity, renewability and the like.

The invention relates to a bacillus subtilis str.TR21 (hereinafter referred to as 'TR21') strain having broad-spectrum antagonism to plant pathogenic fungi, in particular to the bacillus subtilis TR21 strain and a growth inhibition function thereof on the plant pathogenic fungi. The bacillus subtilis TR21 strain provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) in January 20, 2010 with a preservation number of CCTCC No: M2010019, wherein the China Center for Type Culture Collection is located in Wuhan University, Lojia Mountain, Wuchang, Hubei, China. The strain provided by the invention is proved by obtained gene segments 16S rDNA, gyrA and gyrB and BLAST search and comparison on NCBI to be a novel bacillus subtilis strain having relatively higher flat antagonistic activity to a plurality of plant pathogenic fungi, particularly having high antagonism to various soil borne vascular diseases, such as fusarium oxysporum, rhizoctonia solani, alternaria and the like, and having good prevention and control effects shown in fields.

The invention relates to the field of biotechnology, in particular to the kale BoWRKY33 gene and its application in resistance to black spot caused by Alternaria brassicicola. Specifically, the present invention discloses a gene BoWRKY33 for improving the resistance of kale to Alternaria brassicae and a protein encoded by the gene, and also discloses a gene overexpression vector 35Spro::BoWRKY33-YFP, a gene silencing VIGS vector BoWRKY33 ‑PTY.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker which is used for identifying alternaria brassicae of a sand pear variety, and belongs to the technical field of molecular genetic breeding of fruit trees. A method for identifying the alternaria brassicae of the sand pear variety comprises the following steps: (1) extracting a genomic DNA (Deoxyribonucleic Acid) of a to-be-detected pear; (2) carrying out a PCR (Polymerase Chain Reaction) amplification reaction by taking the genomic DNA of the to-be-detected pear as a template and utilizing a primer pair; (3) detecting a PCR amplification product, wherein through sequence alignment, basic group in an S83.0_275630 position is the SNP marker of a high-susceptible variety or a high-resistance variety for identifying the alternaria brassicae of the sand pear variety, and the primer pair comprises a forward primer S83-F, SEQ ID NO.1 and a reverse primer S83-R, SEQ ID NO.2. According to the SNP molecular marker, which is screened by using the method and is used for identifying the resistance to the alternaria brassicae of the sand pear variety, disclosed by the invention, a theoretical foundation is laid for the research on molecular breeding of resistance to the alternaria brassicae and a mechanism of resistance to the alternaria brassicae of sand pears.