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Fenghuang Dancong tea plant endogenous antergic Bacillus sp. ZF04 and its use in biocontrol

A technology of Bacillus and ZF04, which is applied in agricultural biological control, from the endophytic antagonistic bacteria of Fenghuang Dancong tea tree and its application in biological control, can solve the problems that there are not many researches on Fenghuang Dancong tea, and achieve a strong inhibitory effect , the effect of good application prospects

Inactive Publication Date: 2016-09-28
HANSHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Fenghuang Dancong tea mainly focuses on the variety identification, heritage diversity analysis, quality chemical characteristics, physiological activity, quality safety and greening process, and there are not many studies on the endophytic bacteria of Fenghuang Dancong tea tree.

Method used

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  • Fenghuang Dancong tea plant endogenous antergic Bacillus sp. ZF04 and its use in biocontrol
  • Fenghuang Dancong tea plant endogenous antergic Bacillus sp. ZF04 and its use in biocontrol
  • Fenghuang Dancong tea plant endogenous antergic Bacillus sp. ZF04 and its use in biocontrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Isolation, screening and identification of Bacillus sp. ZF04 GDMCC No: 60014

[0039] 1. Isolation and screening of strains

[0040] The bacillus (Bacillus sp.) used in the present invention is sampled from the root of Phoenix Dancong tea tree in Fubin Town, Raoping County, Chaozhou, Guangdong Province by Hanshan Teachers College. The root sample adopts the root of Phoenix Dancong tea tree, collected in Chaozhou, Guangdong Province In Fubin Town, Raoping County, the tree ages are 15, 25 and 50 years old, and the planting altitude is 750m. The tea tree variety is Phoenix Dancong tea narcissus, which is organically planted. Endophytic bacteria in roots were isolated by tissue block culture method, with different culture temperature, pH value and culture medium as enrichment conditions, a batch of well-growing bacterial strains were screened out through optimization, and one strain was selected as ZF04 strains.

[0041] Separation steps:

[0042] (1) Separati...

Embodiment 2

[0057] Example 2: Molecular level identification of Bacillus sp. ZF04 GDMCC No: 60014

[0058] The bacterial DNA genome rapid extraction kit was used to extract the DNA of the target strain.

[0059] The primers are bacterial universal primers, bacterial 16SrDNA universal primer sequence:

[0060] 27F: 5′-AGAGTTTGATCCTGGCTCAG-3′

[0061] 1492R: 5'-GGTTACCTTGTTACGACTT-3'

[0062] Primer 27F, primer 1492R, and 2×TapPCR Master mix used in this application were all purchased from Biological Engineering (Shanghai) Co., Ltd.

[0063] The 20 μL PCR amplification system used to extract genomic DNA: 1.0 μL each of primer 27F and primer 1492R, 7.0 μL of 2×TapPCCR Master mix, 1.0 μL of endophytic bacterial DNA mixture, and 10.0 μL of double distilled water.

[0064] PCR amplification program: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 40s, 30 cycles from denaturation to extension, extension at 72°C for 7 minut...

Embodiment 3

[0068] Example 3: Growth Factors of Bacillus sp. ZF04 GDMCC No: 60014

[0069] Table 3: Effects of temperature, pH and NaCl on the growth of strain ZF04

[0070] temperature(℃)

4

10

15

20

25

30

37

40

45

growing situation

-

+

+

++

++

++

+++

+

+

pH

4

5

6

7

8

9

growing situation

-

+

++

+++

+

-

NaCl concentration

5％

6％

7％

8％

9％

10％

15％

growing situation

+++

++

+

+

+

+

-

[0071] Bacillus sp. (Bacillus sp.) ZF04 GDMCC No: 60014 was cultivated according to the above method. The culture conditions of the bacteria were: the culture medium was beef extract peptone liquid medium and nutrient agar medium, and the culture conditions were pH7.0, temperature 37 Cultivate at ℃ for 20h.

[0072] It can be concluded from Table 3 that the most suitable growth factors for bacte...

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Abstract

The invention discloses Fenghuang Dancong tea plant Bacillus sp. ZF04 and its use in biocontrol. Through Fenghuang Dancong tea plant root sampling, separation purification, screening and culture, Bacillus sp. ZF04 GDMCC No. 60014 with good biocontrol effects is obtained. Physiological and biochemical characteristics and a molecular level of the Bacillus sp. ZF04 are obviously different from those of the common bacillus and is a typical novel strain. The Bacillus sp. ZF04 has good bacteriostasis effects on pear alternaria brassicae, walnut leaf blight pathogens, red date alternaria brassicae and rhizoctonia solani, has mycelia growth inhibition rates of 77.55%, 83.30%, 74.52% and 75.19%, has a fruit pythium hyphae growth inhibition rate of 31.60%, is typical, outstanding and specific biocontrol bacteria and has wide application values in biocontrol.

Description

technical field [0001] The invention relates to the technical field of agricultural biological biocontrol, in particular to the technical field of an endophytic antagonistic bacteria from Fenghuangdancong tea tree and its application in biological control. Background technique [0002] Endophyte refers to a class of microorganisms that survive in various tissues or intercellular spaces of healthy plants during part or all of their life history, without causing obvious pathological symptoms to the host. Antagonistic endophytes refer to microorganisms capable of producing antagonistic effects. Plants are a complex micro-ecological system. Healthy plants contain a large number of endophytic bacteria (Endophytic Bacteria), which have been isolated and identified from more than 50 kinds of plants such as wheat, cotton, rice, peanut, potato, tomato, lemon, and citrus. There are more than 50 genera of endophytic bacteria, and almost all healthy plants contain endophytic bacteria. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 傅力蔡丽王忠合王军侯小桢
Owner HANSHAN NORMAL UNIV
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