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SNP (Single Nucleotide Polymorphism) marker related to resistance to alternaria kikuchiana and application

A resistance-related, pear black spot technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of weak research on anti-black spot gene localization and no SNP markers, etc. Achieve the effect of eliminating the interference of human factors, speeding up the breeding process, and accurate and reliable results

Active Publication Date: 2016-04-20
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although black spot is one of the important diseases, the research on the mapping of black spot resistance genes is relatively weak
The current reports mainly focus on the inoculation identification of pear black spot, and there is no report on the use of SNP markers for assisted selection of pear breeding traits

Method used

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  • SNP (Single Nucleotide Polymorphism) marker related to resistance to alternaria kikuchiana and application
  • SNP (Single Nucleotide Polymorphism) marker related to resistance to alternaria kikuchiana and application

Examples

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Embodiment 1

[0023] The development of SNP markers linked to pear black spot resistance was obtained by:

[0024] 1. Screen pear resistant and sensitive materials, and build a research group for pear black spot resistance genetics

[0025] The pear cultivar 'Jinjing' with high resistance to black spot disease and the variety 'Hongfen' with high susceptibility to black spot disease, which were screened after years of natural disease investigations in the field and identification of artificial inoculation, were selected as parents for hybridization, and 341 F1 plants were obtained.

[0026] 2. Inoculation identification of pear black spot resistance

[0027] Select new shoots with the same leaf age and just fully expanded young leaves for inoculation. Each plant was inoculated with 2 slices and repeated 3 times. After screening, the strain was determined to be the H strain with large spore production, strong pathogenicity and wide distribution, and the spore concentration was 5×10 5 / ml, ...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker which is used for identifying alternaria brassicae of a sand pear variety, and belongs to the technical field of molecular genetic breeding of fruit trees. A method for identifying the alternaria brassicae of the sand pear variety comprises the following steps: (1) extracting a genomic DNA (Deoxyribonucleic Acid) of a to-be-detected pear; (2) carrying out a PCR (Polymerase Chain Reaction) amplification reaction by taking the genomic DNA of the to-be-detected pear as a template and utilizing a primer pair; (3) detecting a PCR amplification product, wherein through sequence alignment, basic group in an S83.0_275630 position is the SNP marker of a high-susceptible variety or a high-resistance variety for identifying the alternaria brassicae of the sand pear variety, and the primer pair comprises a forward primer S83-F, SEQ ID NO.1 and a reverse primer S83-R, SEQ ID NO.2. According to the SNP molecular marker, which is screened by using the method and is used for identifying the resistance to the alternaria brassicae of the sand pear variety, disclosed by the invention, a theoretical foundation is laid for the research on molecular breeding of resistance to the alternaria brassicae and a mechanism of resistance to the alternaria brassicae of sand pears.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic breeding of fruit trees, and provides an SSR molecular marker method for identifying black spot resistance of sand pear varieties, which can be used for early identification of black spot resistance of sand pear varieties and black spot resistance breeding of sand pears, so as to improve The efficiency of sand pear disease-resistant breeding is applicable to scientific research units and companies engaged in pear scientific research, breeding and production. Background technique [0002] The pathogen of pear black spot is Alternariakikuchiana Tanaka of the subphylum Alternaria. The fungus overwinters as conidia and mycelium on the dead branches, diseased leaves, diseased fruits and diseased residues falling on the ground. In the second spring, conidia are produced and spread by wind and rain, causing initial infection from stomata, lenticels and direct invasion of host tissues. After t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 胡红菊张靖国孙中海杨晓平陈启亮范净
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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