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Streptomyces viridocyaneus Sv-29 and application thereof

A technology of Streptomyces cyanobacterium and Streptomyces cyanus, applied in the application, bacteria, fungicides and other directions, can solve the problems of poor stability, slow effect, and drug efficacy is greatly affected by environmental factors, and achieves strong control effect and good antagonistic effect. , the effect of a wide range of applications

Inactive Publication Date: 2013-11-06
昆明保腾生化技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the biological control of these two diseases has progressed rapidly, but the development efforts are still in the stage of experimental exploration, and there are defects such as slow effect, poor stability, and large influence of environmental factors on drug efficacy.

Method used

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  • Streptomyces viridocyaneus Sv-29 and application thereof
  • Streptomyces viridocyaneus Sv-29 and application thereof
  • Streptomyces viridocyaneus Sv-29 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Isolation and identification of bacterial strain Sv-29

[0013] The bacterial strain Sv-29 in the present invention is isolated from the rhizosphere soil of Cotinus sinensis in the suburb of Qujing City, Yunnan Province, and the preservation number is CGMCC NO.7398. The strain identification was determined comprehensively by morphological characteristics, physiological and biochemical characteristics and 16SrDNA sequence comparison analysis.

[0014] Morphological characteristics of the strain Sv-29: the strain grows well on Gao's No. 1 medium, the color of the colony is off-white, the surface is powdery, raised, and has obvious folds. The hyphae in the base are abundant, multi-branched, and do not break. The aerial hyphae are well developed, and when mature, sporocysts are formed until they are spiral; the spore chains are long and differentiate into spores.

[0015] Physiological and biochemical characteristics of strain Sv-29: growth temperature range...

Embodiment 2

[0020] Embodiment 2: the bacteriostatic spectrum determination of bacterial strain Sv-29

[0021] Insert phytopathogenic fungi into the center of the PDA plate, inoculate the strain Sv-29 at a distance of 35 mm from the center on the vertical intersection line of the plate, and cultivate at 28°C to observe the size of the radius of the inhibition zone. Three repetitions were set up for each treatment, and the statistical method used One-Way ANOVA / Daucan in SPSS 19.0 for Windows, and different capital letters indicated that there were significant differences between different treatments at the P=0.01 level.

[0022] The strain Sv-29 has inhibitory effects on all the tested plant pathogenic fungi, and the inhibitory effects on different pathogenic fungi are different, among which the inhibitory effect on Alternaria brassicae and Fusarium oxysporum wilt transformation is the best, and the inhibition zone The radii are 10.8 mm and 11.3 mm, respectively, and the results are shown i...

Embodiment 3

[0025] Embodiment 3: the antibacterial component analysis of bacterial strain Sv-29

[0026] The strain Sv-29 was cultured in PDB medium, and after shaking culture at 28°C for 7 days, the mycelia and supernatant were separated by centrifugation at 7000 r / min for 15 minutes, the mycelium was soaked in 2 times the volume of acetone at 4°C for 24 hr, and the supernatant was washed with Extract with 2 times the volume of dichloromethane or ethyl acetate, concentrate under reduced pressure (vacuum degree 0.07 Mpa), and use gas chromatography-mass spectrometry to analyze the chemical composition of the extract. The instrument model is Agilent6890-5973 N GC- MS, the ion source was EI, the electron energy was 70 Ev, the ion source temperature was 230°C, the interface temperature was 280°C, the mass scanning range was 29-500 amu, and the chromatographic column was HP-5MS (30 m×0. 25 mm× 0. 25 μm), the initial column temperature was 50°C, and then the temperature was raised to 300°C at ...

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Abstract

The invention relates to a streptomyces viridocyaneus Sv-29 and an application thereof, and belongs to the field of biotechnology. The streptomyces viridocyaneus (S. viridocyaneus) Sv-29 is obtained from nature by a general method. The S. viridocyaneus Sv-29 is preserved in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.7398. The S. viridocyaneus Sv-29 can produce various antibacterial active substances, and has good antagonism against various fungal pathogens. Especially, the S. viridocyaneus Sv-29 has stronger prevention and treatment effects on Chinese cabbage alternaria leaf spot caused by alternaria brassicae and cotton fusarium wilt caused by fusarium oxysporum f. sp. vasinfectum.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Streptomyces viridocyaneus Sv-29 and an application thereof. Background technique: [0002] Streptomyces is an important class of microbial resources, and it has been valued for producing a wide variety of biologically active substances with diverse structures. In the research and development of agricultural antibiotics, 80% of the active substances are derived from Streptomyces in the soil, which has the advantages of short cycle, easy research, convenient production, non-toxic and harmless, so excavating and utilizing antibacterial activities in the soil Streptomyces is an important way to develop biopesticides. However, with the extensive and in-depth research, the probability of discovering new compounds becomes lower and lower, and the discovery of new compounds is the basic premise of drug development. The biggest problem in the development of microbia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
Inventor 闫春丽解燕曾嵘林选之陈穗云王建光张廷金孙文向杨发尹忠仁李文荣
Owner 昆明保腾生化技术有限公司
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