42 results about "7-Dehydrocholesterol" patented technology

The invention discloses a preparation method of a 7-dehydrocholesterol, which comprises the following steps: a) protecting a hydroxyl on the 3 position of a cholesterol; b) oxidizing a carbon on the 7 position of the product made in step a) into a carbonyl; c) carrying out hydrazone reaction to the product made in step b) with hydrazine derivatives, the structure of the hydrazine derivatives being at most one H to be substituted by the following hydrazine; d) reacting the product made in step c) with a strong basic reagent having stronger alkaline than a sodium hydroxide; e) reacting the product made in step d) with water or an acid. In the preparation method, no halogen group elements such as Br element are participated, the side effects are less and the yield is high, and the prepared product is safe and innocuous.

The invention belongs to the field of organic photochemistry synthesis, and in particular relates to a method for synthesizing a resinous vitamin D3 by using a micro-flow photoreaction technology, and a micro-flow photochemical reactor for synthesizing the resinous vitamin D3. The method for synthesizing the resinous vitamin D3 by using the micro-flow photoreaction technology comprises the steps of performing illumination reaction in the micro-flow photochemical reactor under the protection of nitrogen (preferably high-purity nitrogen); controlling the temperature of a photochemical reaction solution during reaction to be lower than 28 DEG C, and adjusting and controlling the flow rate of the photochemical reaction solution in a micro-flow pipe by using a peristaltic pump, wherein the conversion rate of 7-dehydrocholesterol after the illumination reaction reaches 90.5-95%, and the HPLC (high-performance liquid chromatography) yield of a pre-vitamin D3 is improved to 70-73.1%. The resinous vitamin D3 is prepared through thermal isomerization reaction, and the HPLC yield of the resinous vitamin D3 is 68-71.1%. The whole preparation process is simple and easy to operate, so that large-scale production can be realized easily.

An implant to be used as medical or dental implant, comprising a metallic or polymeric base which is covered by the vitamin D precursor cholecalciferol. The implant can be obtained by direct covering of the polymeric or metallic base with a solution comprising cholecalciferol or also covering the base with the 7-dehydrocholesterol (7-DHC), and subsequently irradiated with UV light to induce the formation of cholecalciferol. Optionally, the coating of the implant may include an antioxidant such as vitamin E. This implant enhances osseointegration in compromised patients by means of the endogenous synthesis and activity of vitamin D in hard and mineralized tissue regeneration. Furthermore, a method to obtain these implants which comprises coating the surface of the implant directly with cholecalciferol or with a specific concentration of 7-DHC and irradiated with UV light to induce the formation of cholecalciferol.

A photochemical process for the production of previtamin D3 or a derivative thereof from 7-dehydrocholesterol is disclosed. The process includes irradiating the 7-dehydrocholesterol in a reactor with a UV radiation source, wherein the UV source includes an excimer or exciplex emitter which emits quasi-monochromatically according to the corona discharge mechanism in the UV range, and recovering previtamin D3 or a derivative thereof. Previtamin D3 compositions and derivatives made from this process are also provided.

The present invention discloses a preparation method of 7-dehydrocholesterol. The saponified waste from the prior production of vitamin D3 is always treated as waste liquid, which wastes the resources greatly and causes the environmental pollution. The 3-carbonyl holest-4, 6-diene is prepared by the oxidation reaction of the 3-hydroxyl cholest-4, 6-diene vitamin D3 and weak oxidants under the nonpolar or weak polar solvent; then the mixture of 3-carbonyl holest-4, 6-diene and 3-hydroxyl cholest-5, 7-diene is prepared by the reduction reaction of the 3-carbonyl holest-4, 6-diene under weak polar solvent; the crystal of 3-hydroxyl cholest-5, 7-diene and the filtrate rich in 3-hydroxyl cholest-4, 6-diene is obtained after recrystallization purification of the mixture, and the filtrate can be used as materials of oxidation reaction after eliminating the solvent. The present invention prepares the 7-dehydrocholesterol with saponified waste, thereby the saponified waste is used efficiently and the environmental pollution is reduced.

Provided herein are enzymatic methods of producing steroid compounds such as 7-dehydropregnenolone, hydroxy derivatives thereof and vitamin D2- and D3-like compounds and derivatives thereof. Also provided are the derivatives of the vitamin D3-like compounds so generated via the action of cytochrome P450scc enzyme on substrates 7-dehydrocholesterol, vitamin D2 or vitamin D3.

The invention relates to the field of organic synthesis, and discloses a method for preparing acetyl cholesterol-7-one p-toluenesulfonylhydrazone. The method comprises the following steps: carrying out a contact reaction on acetyl cholesterol-7-one and p-methylbenzenesulfonhydrazide under a condensation reaction condition in a solvent containing cation exchange resin. According to the method for preparing the acetyl cholesterol-7-one p-toluenesulfonylhydrazone, acetyl cholesterol-7-one p-toluenesulfonylhydrazone with a relatively high content can be obtained under mild reaction conditions, sothat the yield of subsequent preparation of 7-dehydrocholesterol is improved. Meanwhile, a catalyst in the method is easy to recycle after the reaction, so that overall cost is reduced.

The present invention relates to a 25-hydroxy-7-dehydrocholesterol synthetic method, a 25-hydroxy-7-keto-cholesterol derivative is used as a raw material for mechanical milling reaction in a ball mill pot under the effect of sulfonyl hydrazide and a base in the absence of an organic solvent, and then hydrolysis reaction is performed to obtain 25-hydroxy-7-dehydrocholesterol synthetic method. According to the method, in the absence of a solvent, mechanical milling reaction is performed, no dust is spilled over in the grinding process, reaction controllability is good, reaction efficiency is high, the solvent recovery operation in the post-processing process can be avoided, operation is simplified, environmental pollution is reduced, the reaction selectivity is good, yield is high, operation is simple, cost is low, post-treatment is simple, three-waste is less, product is easy to separate, purity is high, the yield is up to 89%, the melting process is short, and the product purity is high.

The invention relates to a method for preparing 7-dehydrocholesterol, which comprises the following steps: 1) protecting hydroxyl on the 3-site of cholesterol; 2) oxidizing carbon on the 7-site of the product obtained in step 1) into carbonyl; 3) carrying out a hydrazone reaction on the product obtained in step 2) and a hydrazine derivative; 4) reacting the product obtained in step 3) with a strongly alkaline reagent; and 5) reacting the product obtained in step 4) with water or an acid. The method has the advantages of simple operation process, less secondary reaction, high yield and less corrosion to equipment, thereby having high industrial production values.

The invention discloses a method for preparing vitamin D3 and used equipment. In the prior method, a solvent must be replaced during the crystallization process of the post-treatment, and the heating time is long, which affect the content of a final product. A solvent used by the method in a photochemical reaction is a mixed solvent consisting of a high melting point solvent and a low melting point solvent; an obtained photochemical reaction liquid is subjected to freezing crystallization and filtering post-treatment after the photochemical reaction is finished; and crude vitamin D3 oil is obtained after filtrate is concentrated; after a filter cake is heated and dissolved, 7-dehydrocholesterol and the low melting point solvent which is concentrated from the filtrate are added to serve as raw materials for the photochemical reaction; the high melting point solvent is alcoholic solvents or hydrocarbon solvents with melting points of between 10 DEG C below zero and 10 DEG C, and the low melting point solvent is alcohol solvents with melting points less than or equal to 50 DEG C below zero. The vitamin D3 obtained by the method has high content and less polymerization side reactions, does not need to replace the solvent during the post-treatment process, and has good quality.

The present invention relates to a PEGylated 7-dehydrocholesterol derivative, and a composition for wrinkle alleviation and antiaging, containing the same. The PEGylated 7-dehydrocholesterol derivative, according to the present invention, has improved water solubility and stability, and thus can be effectively used, as an excellent vitamin D source, in a cosmetic composition, a pharmaceutical composition, a dietary supplement and the like for wrinkle alleviation and antiaging.

The present invention provides, in certain aspects, novel 7-dehydrocholesterol (7DHC) derivatives that are useful in treating or preventing cancer, as well as in treating or preventing uncontrolled angiogenesis, in a subject. In certain embodiments of the present invention, the subject is a human. In other aspects, the present invention provides a method of preparing compounds of the invention, or a salt or solvate thereof.

Provided herein are enzymatic methods of producing steroid compounds such as 7-dehydropregnenolone, hydroxy derivatives thereof and vitamin D2- and D3-like compounds and derivatives thereof. Also provided are the derivatives of the vitamin D3-like compounds so generated via the action of cytochrome P450scc enzyme on substrates 7-dehydrocholesterol, vitamin D2 or vitamin D3.