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37results about How to "Sampling is simple" patented technology

Simultaneous localization and mapping method based on distributed edge unscented particle filter

The invention relates to a simultaneous localization and mapping method based on distributed edge unscented particle filter. First, a coordinate system is built and an environmental map is initialized; then subfilters are built for each landmark point with successful matching respectively; next, based on a robot motion model, a particle swarm is generated in each subfilter respectively, and the state vector and the variance of each particle are obtained; noise is introduced, particle state vectors after extension are calculated by utilization of unscented transformation, the particles after extension are updated and the particle swarms are optimized; then particle weights are calculated and normalization is carried out, and aggregated data of each subfilter are subjected to statistics and the data are sent to a master filter; next, global estimation and variance are calculated; then the effective sampling draw scale and sampling threshold of each subfiter are determined, the subfilters with severe particle degeneracy are subjected to resampling; then the state vectors and the variances of the robot are output, and stored in a map. Finally, landmark point states are updated by utilization of kalman filtering algorithm until the robot is no longer running.
Owner:BEIJING UNIV OF TECH

Completely split type rapid ecological soil sampler

The invention relates to an?ecological soil sampler, and in order to solve the problems of difficult sample output after sampling, easy disturbance of soil layers, easy change of soil biological patterns and easy biomass loss existing in existing soil collectors, the invention further provides a completely split type rapid ecological soil sampler. Two plug pins are inserted into two jacks correspondingly to fixedly connect a first semicylindrical body and a second semicylindrical body into a complete sampling cylinder. Two inverted L-shaped sockets that are centrosymmetric with respect to a socket pipe cap central axis are processed on the lower outer wall of a socket pipe cap. A handle is in threaded connection with the upper end of an upright pipe through a second pipe hoop, and the upright pipe lower end is fixedly connected to the center of the socket pipe cap upper end. The upper end of the sampling cylinder is sheathed on the lower end inner wall of the socket pipe cap, and a first cylindrical pin and a second cylindrical pin are respectively clamped in the two inverted L-shaped sockets of the socket pipe cap. The ecological soil sampler provided in the invention is used for collecting ecological soil samples.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Immunochromatography reagent strip for quantitative detection of 25-hydroxyvitamin D and preparation method of immunochromatography reagent strip

The invention relates to the technical field of biology, and discloses an immunochromatography reagent strip for quantitative detection of 25-hydroxyvitamin D and a preparation method of the immunochromatography reagent strip. The immunochromatography reagent strip for quantitative detection of the 25-hydroxyvitamin D comprises a sample adding region, a conjugate release region, a reaction region and a water absorption region, which are sequentially arranged; the conjugate release region is a glass cellulose membrane enveloped with colloidal gold or a quantum dot or an up-conversion luminescent material UCP-marked mouse-anti-human 25-hydroxyvitamin D monoclonal antibody; the reaction region is a test region and a control region; the test region is enveloped with 25-hydroxyvitamin D-coupling protein; and the control region is enveloped with a nitrocellulose membrane of a goat anti-mouse IgG antibody. The immunochromatography reagent strip is capable of accurately, quantitatively, rapidly and sensitively detecting the 25-hydroxyvitamin D in human whole blood, serum and plasma; the required instrument is different from a traditional large expensive instrument for a clinical laboratory; and only one corresponding miniature immunity analyzer is required, therefore, real-time detection is realized.
Owner:PRO MED BEIJING TECH

Method for detecting Clenbuterol residual quantity in hair

InactiveCN101458238ADynamic monitoring of the history of illegal abuseEnsure safetyComponent separationAcid waterSmall sample
The invention discloses a method for detecting clenbuterol residue in hair. The method sequentially comprises the following steps: (1) pre-processing a hair sample: hydrolyzing the sample with alkali at a high temperature, extracting the sample with an organic solvent and reversely extracting the sample with acid water; and (2) taking deuterated clenbuterol-D9 aqueous solution with the concentration of 5-500ng / ml as an internal standard, and detecting the clenbuterol residue in hair by liquid chromatogramy-tandem mass spectrometry. The method has the following advantages: 1. hair is taken as a detection material, which provides easy sampling, convenient storage and repeatable sampling, sampling is simple and rapid and has trace amount and high efficiency; 2. the pre-processing method of the hair sample is optimized, which shortens the pre-processing time, simplifies the processing steps and obviously reduces the cost; and 3. an isotopic compound is taken as the internal standard, and the liquid chromatogramy-tandem mass spectrometry is used as a detection means, the minimum detection limit reaches 0.01ng / g, and the minimum quantification limit is 0.5ng / g. The method has the accurate and reliable capacity to qualitatively and quantitatively detect the clenbuterol, and can be taken as a tool for large sample screening or small sample confirmation.
Owner:CENT HOSPITAL XUHUI DISTRICT SHANGHAI CITY

Real-time graph generation method for scene matching navigation technology

The invention relates to the field of navigation and guidance and relates to a real-time graph generation method for scene matching navigation technology. The real-time graph generated by the method can simulate the influence of various major error sources, including the influence of attitude and height error of an airplane, the noise interference of an imaging system and the parameter error interference of a CCD camera; and data in the generated real-time graph closely approximates real flight data. The method can provide abundant reliable test data for the research and development of the scene matching navigation technology and can sufficiently evaluate and verify the performance indexes of various system algorithms, thereby having important use value.
Owner:FLIGHT AUTOMATIC CONTROL RES INST

Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip

The invention discloses an immunochromatographic reagent strip, and particularly relates to an immunochromatographic reagent strip for detecting PCT (procalcitonin) and a preparation method of the reagent strip. A detection line is arranged on immunochromatographic test paper, and is formed by coating of an antibody, the antibody is selected from a mouse anti-human PCT monoclonal antibody, and a quality control line is formed by coating of a sheep anti-mouse IgG polyclonal antibody; the mouse anti-human PCT monoclonal antibody and colloidal gold are prepared into a colloidal gold combined pad. The preparation method of the immunochromatographic reagent strip for detecting the PCT is characterized by comprising the following steps: A, coating a nitrocellulose membrane with a detection line working solution and a quality control line working solution of each antibody, drying the obtained nitrocellulose membrane at a temperature of 36-38 DEG C for 8-12 hours to obtain a chromatographic membrane; B, preparing the mouse anti-human PCT monoclonal antibody and the colloidal gold into the colloidal gold combined pad; and C, sequentially adhering a sample pad, the colloidal gold combined pad, the chromatographic membrane and a water absorbing pad on a rubber plate to prepare the immunochromatographic reagent strip for detecting the PCT.
Owner:管义东

Automatic gain control apparatus and method in wireless telecommunication system based on time division duplex

InactiveCN101375510AOptimal Receive SensitivityOptimal reception strengthGain controlTransmissionCommunications systemSignal on
Disclosed is an apparatus and a method for controlling automatic gain in a wireless communication system using a Time Division Duplex (TDD) scheme. The apparatus comprises an power detecting unit for detecting a power of a TDD frame signal on a receive path; an Automatic Gain Control (AGC) synchronizing signal generating unit for generating an AGC synchronizing signal enabled or disabled either during a Down Link (DL) frame or during an Up Link (UL) frame of a TDD frame; an automatic gain calculating unit for calculating a gain control value based on the power detected by the power detecting unit either during the DL frame or during the UL frame of the TDD frame in response to the AGC synchronizing signal; and an AGC unit for performing gain control for the TDD frame signal on the receive path on the basis of the calculated gain control value. Hence, as AGC is performed during only an actual receive frame, the optimized demodulation performance can be maintained.
Owner:POS DATA CO LTD

Infrared test approach for venomous injurant acute toxicity algae

The invention provides an acute toxicity algae infrared testing method of toxic and harmful substances, which adopts the sensitive algae liquid with the chlorophyll content not less than 4.0mg.L-1 and the toxicity test factors of a portable infrared thermometer. The contrast toxicity test is carried out by selecting the processing and concentration design of a test sample, the response dose and the minimum response concentration of the sensitive algae to the sample are determined by the sample dilution concentration and the adding amount of a first-appeared temperature response during the dilution concentration test of using different samples for response temperature difference and response time so as to determine the toxicity of the sample. The sensitivity of the method of the invention can achieve the sensitivity of a luminous bacteria method, the average testing time of the method and the average testing time of the luminous bacteria method are similar, the testing effect ofthe acute toxicity algae infrared testing method of the toxic and harmful substances is the same with the testing effect of the luminous bacteria method. The invention is characterized by convenient test, low cost, strong specificity, field test and so on.
Owner:CHONGQING UNIV

Sampling apparatus and sampling method for simultaneous collection of exhaust gas pollution source size-fractionated particles and gas phase organic matters

The invention provides a sampling apparatus and a sampling method for simultaneous collection of exhaust gas pollution source size-fractionated particles and gas phase organic matters. The sampling apparatus comprises the following components: a multifunctional sampling pipe which is connected to a flue; an Andersen fractionation sampler which is connected to the multifunctional sampler, wherein the diameter of an air intake of the Andersen fractionation sampler and the diameter of an inner pipe of the multifunctional sampling pipe are identical; an outlet end of the Andersen fractionation sampler which is connected to a quartz fiber filter membrane cylinder; a condensation unit which is connected to an outlet end of the quartz fiber filter membrane cylinder; an ion exchange adsorption resin unit which is connected to an outlet of the condensation unit; a drying unit which is connected to the ion exchange adsorption resin unit; and a sampling instrument host which is connected to the drying unit. The sampling apparatus can be used for simultaneously collecting the exhaust gas pollution source size-fractionated particles and the gas phase organic matters; equipment in the sampling apparatus has the advantages of easy connection, easy operation, simple and easy sampling, good effects, high accuracy, and environmental and social dual benefits.
Owner:UNIV OF SCI & TECH OF CHINA

Simple and easy flow equalization scheme of parallel system

The invention provides a simple and easy flow equalization scheme of a parallel system. The parallel system consists of power supply modules with multiway switches in parallel connection, the flow equalization scheme adopts an independent PWM (Pulse Width Modulation) controller, output currents of all the power supply modules are sampled and fed back to the PWM controller, and output voltage of all the power supply modules is regulated by the PWM controller, so that the flow equalization of the output of all the power supply modules is achieved. The flow equalization scheme is characterized in that sampling of the output currents of all the power supply modules is realized through detecting voltages at both terminals of a capacitor C in an RC (Remote Control) network. For the flow equalization scheme of the parallel system, when the output currents of all the power supply modules are sampled, the sizes of a load current and an inductive current are detected, only the size of the voltage of the capacitor in the RC (Remote Control) network is required to be detected, by the method, the currents are conveniently, simply and easily sampled without depletion, the cost is low, and current distortion is not caused.
Owner:GUANGXI UNIV

Audio and video transmission system based on quantum encryption

The invention discloses an audio and video transmission system based on quantum encryption. The system comprises a first quantum key generation module, a second quantum key generation module, a quantum key transfer module, a file storage module, an audio and video extraction module, an audio encryption module, an audio decryption module, a video sampling module, a video coding module, a video decoding module, a video encryption module and a video decryption module. The method has the beneficial effects that the two quantum key generation modules are respectively used for encrypting and decrypting audios and videos, so that the high requirement of simultaneously transmitting the audios and the videos in one file by utilizing one quantum key generation module on the coding speed of the quantum key in the prior art is reduced.
Owner:江苏宇太网智科技有限公司

Three-phase driving bridge lower bridge arm three-resistor based motor current sampling method and system

The invention proposes a three-phase driving bridge lower bridge arm three-resistor based motor current sampling method and system. Current sampling is triggered according to periodic change of a PWMdriving signal, a section where a motor rotor is currently located is simultaneously judged, current sampling is performed by combining the characteristic of a central edge aligned to the PWM modulation signal and distribution characteristic of each phase voltage at each section, the current sampling accuracy is high, the voltage utilization efficiency is high, moreover, no complicated circuit isused, the cost is low, soft implementation is easy, and a few CPU resources are occupied.
Owner:ZHUZHOU ELITE ELECTRO MECHANICAL

Device and method for testing fogdrop content in smoke gas

The invention discloses a device for testing fogdrop content in smoke gas and a method for testing fogdrop content in smoke gas. The device for testing fogdrop content in smoke gas comprises a fogdrop absorbing bottle, a pitot tube, an acid gas absorbing bottle, a dryer, an intelligent dynamic pressure balance smoke dust sampling pump and a connecting pipe between the fogdrop absorbing bottle and the pitot tube, and a connecting pipe between the pitot tube and the intelligent dynamic pressure balance smoke dust sampling pump. The device applies absorption liquid to absorb fogdrop with all grain diameters in the smoke gas, thus the sampling bias is reduced; through measuring the Mg2+ content in the absorption liquid, the fogdrop content is directly obtained; the fogdrop absorbing bottle is arranged at the head part of the pitot tube, and extended to a smoke flue in measurement to sample; the device and method for testing fogdrop content in smoke gas can absorb the fogdrop in the smoke gas by the absorption liquid, thus the sampling bias is reduced; through measuring the Mg2+ content in the absorption liquid, the fogdrop content is directly obtained; the method and device are convenient and fast.
Owner:HUADIAN ELECTRIC POWER SCI INST CO LTD

Sampling device and sampling method for massively collecting atmospheric gaseous mercury in short term

The invention discloses a sampling device and sampling method for massively collecting atmospheric gaseous mercury in a short term. The sampling device comprises a filter membrane box for filtering away atmospheric particulate matter, an atmospheric gaseous mercury sampling unit connected with an outlet of the filter membrane box, an instantaneous flow meter connected with the outlet end of the atmospheric gaseous mercury sampling unit, a vacuum pump connected with the outlet end of the flow meter, and a volumetric flow meter connected with an outlet of the vacuum pump, wherein the atmosphericgaseous mercury sampling unit is composed of six gold-plated quartz sand tubes and two modified conical funnels. The sampling device is capable of collecting a sufficient amount of atmospheric gaseous mercury for isotope analysis within a few hours; and equipment in the sampling device is easy to assemble and disassemble and easy to operate; sampling is simple and easy; and the accuracy is high.
Owner:TIANJIN UNIV

Standard clamp rail type intelligent photovoltaic battery pack string monitoring device

The invention discloses a standard clamp rail type intelligent photovoltaic battery pack string monitoring device. The device adopts a milliohm divertor and one sampling channel of a dual-channel analog-digital converter ADC to sample the stringing current, adopts a voltage sampling resistor and another sampling channel of the dual-channel analog-digital converter ADC to sample the stringing voltage, adopts a build-in temperature sensor of the dual-channel analog-digital converter ADC to measure the working temperature of the fuse, and adopts a self-excited isolated power source to supply power. The device of the embodiments of the invention can be used to overcome various defects in the prior art, can be used to monitor various parameters, such as the photovoltaic battery pack string voltage, current, fuse state, temperature, etc., and has advantages of high acquisition accuracy, no current measurement zero offset, small temperature drift, high accuracy voltage measurement, small occupied space and easy installation.
Owner:SHENZHEN GOLDEN HIGHWAY TECH CO LTD

A kind of udp network protocol realizes the device and method of displaying electrocardiogram in browser

The invention discloses a device and a method for realizing electrocardiogram display of a browser by a UDP (User Datagram Protocol) network protocol, and the device comprises an electrocardiogram sampling data sending device consisting of a NODEMCU (Non-Oriented Digital Enhanced Microprogrammed Control Unit) module, an AD623 instrumentation amplifier and an anti-interference electrocardiogram amplification circuit realized by a broadband pass filter consisting of two stages of 358 operational amplifiers. The NODEMCU module is wirelessly connected with a mobile phone or a computer through a built-in WIFI network, and a NodeJS UDP-to-WS protocol is configured on the mobile phone or the computer, so that the single-chip microcomputer can display a transverse wide dynamic electrocardiogram onthe mobile phone or the computer through UDP protocol network communication. Implementation of an HTML5-JS program at a browser end is a mainstream front-end technology, so that the programming environment requirement is reduced to the minimum, and the invention is particularly suitable for popularization and open sources. The mode has the greater significance that data processing and storage management are put on a mobile phone or a computer with stronger processing capacity and ultra-large storage capacity, so that the combination of the WIFI single chip microcomputer and the mobile phone or the computer makes the best of each other, and a new WIFI single chip microcomputer application mode is provided.
Owner:ZHEJIANG UNIV

Seal device with lid

The present invention relates to a kind of covered sealer, used especially in water processing facility to collect exhausted waste gas. By setting up a movable combined sealing top cover capable of being opened out and dranw in and easy to install and disassemble, the opened part of the structural unit in water processing facility may be sealed for waste gas collection, sampling and processing of waste gas or opened for service, overhaul and replacement of equipment and member inside the structural unit and observation of the equipoment running state.
Owner:CHINA PETROLEUM & CHEM CORP +1

Method and device for measuring soil potential radon concentration using electrostatic collection method

The invention provides a method and a device for sampling and measuring surface soil to obtain soil potential radon concentration by using an electrostatic collection emanometer. The method comprisesa sampling process and a measuring and calculating process, and can quickly obtain an accurate soil potential radon concentration by a simple calculation method. A measurement system is closed by a sealing cover to form a closed loop gas path measurement device to improve the accuracy of the measurement. When measuring the production rate of radon and soil porosity for sampled soil, the decay, leakage and anti-diffusion effects of the radon are taken into consideration. An electrostatic collection manometer is used, the concentration of the radon is deduced reversely by the concentration of Po-218, and the accurate soil potential radon concentration is quickly calculated by using a nonlinear fitting algorithm, which saves measurement time since it is needless to wait for the concentrationof the radon in a measurement device to be stable.
Owner:HENGYANG NORMAL UNIV

A method and device for measuring soil potential radon concentration by electrostatic collection method

The invention provides a method and a device for sampling and measuring surface soil to obtain soil potential radon concentration by using an electrostatic collection emanometer. The method comprisesa sampling process and a measuring and calculating process, and can quickly obtain an accurate soil potential radon concentration by a simple calculation method. A measurement system is closed by a sealing cover to form a closed loop gas path measurement device to improve the accuracy of the measurement. When measuring the production rate of radon and soil porosity for sampled soil, the decay, leakage and anti-diffusion effects of the radon are taken into consideration. An electrostatic collection manometer is used, the concentration of the radon is deduced reversely by the concentration of Po-218, and the accurate soil potential radon concentration is quickly calculated by using a nonlinear fitting algorithm, which saves measurement time since it is needless to wait for the concentrationof the radon in a measurement device to be stable.
Owner:HENGYANG NORMAL UNIV

Preparation method and application of human pluripotent stem cell senescence model

The invention relates to the technical field of biomedicine, and provides a preparation method and application of a human pluripotent stem cell senescence model. The preparation method of the human pluripotent stem cell senescence model comprises the steps that peripheral blood monocytes from Chinese mutant HGPS patients are reprogrammed into Chinese HGPS-iPSC; c.1579C)T(p. R527C) mutation of LMNAis repaired by using a single nucleotide mutation repair technology mediated by CRISPR / Cas9 and ssODNs; normal iPSC, Chinese HGPS-iPSC and mutation correction iPSC are differentiated into functionalMSCs respectively in vitro, and the expression, cell proliferation, apoptosis, cell cycle, cell senescence and the like of LMNA protein in three iPSC-MSCs are detected, thereby disclosing the effect of the c.1579C)T(p. R527C) mutation on an expression function of cell-related passage signaling molecules, and studying the molecular mechanism and pathogenic mechanism of the Chinese HGPS which are different from Caucasian population mutant HGPS.
Owner:深伦生物科技(深圳)有限公司

Double-sampling single-refreshing method based on three-phase two-level topological structure

ActiveCN114257102ACause distortionEliminate Digital Control LatencyEfficient power electronics conversionAc-ac conversionCapacitanceA wave amplitude
The invention discloses a double-sampling single-refreshing method based on a three-phase two-level topological structure, and the three-phase two-level topological structure comprises a three-phase fully-controlled rectifier bridge, a DC bus capacitor, a three-phase fully-controlled inverter bridge, an LCL filter circuit at a rectification side, an LCL filter circuit at an inverter side, and a series transformer coupled with a power grid voltage. The controller DSP generates a modulation wave through operation and generates a PWM signal after PWM modulation to control the on-off state of the three-phase full-control inverter bridge, specifically, the controller DSP generates the modulation wave through operation, a comparison threshold value of the amplitude of the modulation wave is set in the controller DSP, the modulation wave is sampled at the wave crest and the wave trough of a carrier wave, and the carrier wave is sampled at the wave crest and the wave trough of the carrier wave; and comparing a modulation wave amplitude value obtained by actual sampling with a comparison threshold value to determine a refreshing loading moment. According to the method, the problem of digital control delay is effectively eliminated, and the adverse effect of control delay on the system is solved.
Owner:SUZHOU AIKE BORUI POWER SUPPLY TECH

Animal tissue sampler

The invention relates to animal disease prevention and control equipment, in particular to an animal tissue sampler, which comprises a first arm handle, a secondary arm handle, an upper jaw and a lower jaw, wherein the first arm handle and the lower jaw are integrated into one body, the secondary arm handle and the upper jaw are integrated into one body, the first arm handle and the secondary arm handle are connected by a rotary shaft, a cutter is fixedly arranged on the lower part of the lower jaw, a through hole corresponding to the cutter in term of position is arranged on the lower jaw, a baffle and a pipe clamp are fixed on the lower part of the lower jaw, the right end of the baffle is fixedly connected with a pushing device, at least one sampling pipe is arranged between the pushing device and the right end of the pipe clamp, the upper part of the pipe clamp is fixed with two side surfaces of the lower jaw, a clamping groove is formed in the middle of the right side of the pipe clamp, the sampling pipes are arranged in the clamping groove, and the sampling pipe on the right end of the pipe clamp is corresponding to the through hole in term of position. The animal tissue sampler can simply, quickly, conveniently and easily sample, with less stress on animals, and the tissue samples are normative and unified, and easy to collect without pollution.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI

Lens assembly, device for detecting attachment magnitude and household appliance

ActiveCN105628573ASampling is simpleIncreased reflected light rangeParticle suspension analysisPhysics
The invention discloses a lens assembly, a device for detecting the attachment magnitude and a household appliance. The lens assembly comprises a lens and a surface mesh part and is arranged on the device for detecting the attachment magnitude; a light transmitting surface is arranged on one side of the lens, and an incident surface and an emergent surface are arranged on the other side of the lens; the surface mesh part and the light transmitting surface are spaced with each other and are oppositely arranged, and a plurality of meshes are formed in the surface mesh part. The lens assembly disclosed by the invention is easy to sample and can increase the scope of the quantity of light reflected by attachments.
Owner:GUANGDONG MIDEA KITCHEN APPLIANCES MFG CO LTD +1

Synchronous Localization and Map Construction Method Based on Distributed Edgeless Particle Filter

The invention relates to a simultaneous localization and mapping method based on distributed edge unscented particle filter. First, a coordinate system is built and an environmental map is initialized; then subfilters are built for each landmark point with successful matching respectively; next, based on a robot motion model, a particle swarm is generated in each subfilter respectively, and the state vector and the variance of each particle are obtained; noise is introduced, particle state vectors after extension are calculated by utilization of unscented transformation, the particles after extension are updated and the particle swarms are optimized; then particle weights are calculated and normalization is carried out, and aggregated data of each subfilter are subjected to statistics and the data are sent to a master filter; next, global estimation and variance are calculated; then the effective sampling draw scale and sampling threshold of each subfiter are determined, the subfilters with severe particle degeneracy are subjected to resampling; then the state vectors and the variances of the robot are output, and stored in a map. Finally, landmark point states are updated by utilization of kalman filtering algorithm until the robot is no longer running.
Owner:BEIJING UNIV OF TECH

Excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application thereof

ActiveCN114088855AHigh sensitivity for early clinical detectionExpand clinical applicationComponent separationProteomicsMetaboliteTibial dyschondroplasia
The invention belongs to the technical field of biological detection and diagnosis, and particularly relates to an excrement biomarker for early diagnosis of broiler tibial cartilage dysplasia and application of the excrement biomarker. According to the present invention, differential metabolite 4-hydroxybenzaldehyde capable of distinguishing TD broilers and healthy broilers is obtained through screening based on a metabonomics method and can be used for indicating early TD of the broilers; compared with an existing TD clinical diagnosis method, the method has the advantages that the excrement sample is innovatively selected for clinical detection of a non-invasive biomarker, and the purposes of non-invasion, simplicity, convenience and no stress reaction are achieved; a sample is easy to sample, the detection sample can be collected under a stress-free condition, the stress reaction of the broiler chicken is avoided, and the clinical diagnosis of TD can be simply, conveniently and non-invasively carried out; the excrement biomarker has high sensitivity to early diagnosis of TD, can detect the sub-clinical disease broilers, and is beneficial to intervention and treatment at the early stage of disease.
Owner:HENAN AGRICULTURAL UNIVERSITY

Method for detecting Clenbuterol residual quantity in hair

InactiveCN101458238BDynamic monitoring of the history of illegal abuseEnsure safetyComponent separationSmall sampleAcid water
The invention discloses a method for detecting clenbuterol residue in hair. The method sequentially comprises the following steps: (1) pre-processing a hair sample: hydrolyzing the sample with alkali at a high temperature, extracting the sample with an organic solvent and reversely extracting the sample with acid water; and (2) taking deuterated clenbuterol-D9 aqueous solution with the concentration of 5-500ng / ml as an internal standard, and detecting the clenbuterol residue in hair by liquid chromatogramy-tandem mass spectrometry. The method has the following advantages: 1. hair is taken as a detection material, which provides easy sampling, convenient storage and repeatable sampling, sampling is simple and rapid and has trace amount and high efficiency; 2. the pre-processing method of the hair sample is optimized, which shortens the pre-processing time, simplifies the processing steps and obviously reduces the cost; and 3. an isotopic compound is taken as the internal standard, and the liquid chromatogramy-tandem mass spectrometry is used as a detection means, the minimum detection limit reaches 0.01ng / g, and the minimum quantification limit is 0.5ng / g. The method has the accurate and reliable capacity to qualitatively and quantitatively detect the clenbuterol, and can be taken as a tool for large sample screening or small sample confirmation.
Owner:CENT HOSPITAL XUHUI DISTRICT SHANGHAI CITY
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