The present invention relates to a serum-free full
suspension culture method of duck tempusu viruses and an application of the duck tempusu viruses in vaccines. The serum-free full suspension culturemethod comprises the following steps: a duck tambusu
virus strain is continuously passaged to
virus-adapted cells in a suspension BHK-21
cell line and the adapted
virus strain with the highest virus content is selected as a basic
virus strain; after the suspension BHK-21 cells are resuscitated, a serum-free culture solution is used in a
bioreactor for amplified culture; the suspension BHK-21 cellsafter the amplified culture are inoculated with the basic
virus strain for continuous culture; glucose monitoring is performed every day after
virus inoculation, when glucose in the culture solutionis 1-5 mmol / L, a
nutrition package is supplemented; 48 hours after the
virus inoculation, samples are taken every other 6 hours to calculate
cell viability and when the
cell viability is less than 50%, the virus culture solution is harvested; and the virus culture solution is repeatedly frozen, thawed, centrifuged and stored. The suspension BHK-21
cell culture is used, the viruses have good adaptability, virus content is high,
operating cost is low, product quality is stable, and the method is easy to operate and has feasibility of large-scale industrial production.