The invention discloses an e-commerce express cabinet. The cabinet comprises a cabinet body, an industrial personal computer, an operation panel, a payment device, first disinfection equipment, seconddisinfection equipment, third disinfection equipment, a proximity switch, a detection device and a measurement device, the cabinet body is provided with a storage grid, an operation panel grid and ameasurement grid; the storage grid, the operation panel grid and the measurement grid are respectively provided with electric doors for sealing and covering openings of the storage grid, the operational panel grid and the measurement grid, the operation panel, the payment device and the first disinfection equipment are arranged in the operation panel grid, the measurement device and the second disinfection equipment are arranged in the measurement grid, the third disinfection equipment is arranged in the storage grid, and the proximity switch is arranged on the outer wall of the cabinet body to control the door of the operation panel equipment. The invention further discloses an express sending method and an express delivery and pickup method adopting the e-commerce express cabinet. According to the e-commerce express cabinet, the express sending method and the express delivery and pickup method thereof, contact transmission and droplet transmission of viruses in the express receivingand pick-up process can be effectively blocked, and the health of express sending and pickup personnel and deliverymen is guaranteed.