The invention discloses a single-light-source implantable nerve multipoint synchronous interaction chip. The single-light-source implantable nerve multipoint synchronous interaction chip comprises a substrate, a first insulation isolation layer, a second insulation isolation layer, an excitation light path layer and a single light source emitting module, wherein a recording circuit layer is arranged in the middle of the first insulation isolation layer, the first insulation isolation layer is coated on the surface of the recording circuit layer, the excitation light path layer is fixedly connected onto the top surface of the first insulation isolation layer, the first insulation isolation layer and the excitation light path layer form an interaction layer, the opposite two ends of the interaction layer respectively are extension ends extending out of the edge of the substrate, the second insulation isolation layer is fixedly connected onto the bottom surface of the substrate and the bottom surfaces of the two extension ends of the interaction layer, the single light source emitting module is located on the interaction layer, and one extension end of the interaction layer forms a comb-teeth-shaped probe set. According to the chip with the structure, due to the introduction of a beam splitting type multipoint excitation probe, the number of required light sources is reduced, the energy consumption and the calorific value are reduced, and the stability of continuous operation is improved. Meanwhile, the invention further discloses a preparation method of the single-light-source implantable nerve multipoint synchronous interaction chip, which is simple and feasible.