The invention relates to a preparation method of a eurotium cristatum
mycelium extract, and mainly relates to a process for liquid
fermentation of eurotium cristatum, collection of
mycelium and extraction of active ingredients. A
fermentation culture medium of the eurotium cristatum uses tea
powder as a nutritional ingredient and a dispersion medium to promote the
pelletizing property of the eurotium cristatum
mycelium, so that the nutritional ingredients in the culture medium are used and converted to the maximum. Through
fermentation culture in a 1L fermentation tank, the
dry weight of the mycelium can be up to 15g / L. The fermentation liquor is centrifuged to collect the mycelium, then the mycelium is subjected to weight suspending by using water and then is twisted into fragments, a wall-breaking
enzyme is added and uniformly mixed with the mycelium, and then the mixture is subjected to high-pressure homogenization and
alcohol precipitation to extract the active ingredients in the mycelium. The preparation method improves the existing relative processes, so that the extraction rate of the active ingredients in the mycelium is improved, and large-scale production can be realized. The active ingredients in the mycelium mainly comprise
polysaccharide, and have better effects on reducing fat and improving the
stomach and intestines.