The invention discloses a preparation method of sea buckthorn fruit aerosol. Sea buckthorn fruits serve as main raw materials, deltoid goldthread rhizomes, dandelion herbs, immature flowers of dasystyle honeysuckle, wild mint herbs, yanhusuo rhizomes, ural licorice roots, Chinese galls, weeping forsythia fruits, dahurian angelica roots, great burdock fruits and spreading hedyotis herbs are added,and the raw materials are heated, cooked, deslagged, filtered, concentrated, cooled and mixed with borneol according to formula ratio to prepare the sea buckthorn fruit aerosol. The sea buckthorn fruit aerosol is unique in compatibility, directly enters a focus in a spraying mode, solves the problems of poor buccal taste and patient adaptability of an existing medicine, has the functions of diminishing inflammation, inhibiting bacteria, clearing heat and removing toxicity, can enhance immunity, can promote mucosa and wound healing and has special therapeutic effects on oral ulcer, swelling andpain in throat and wind-fire toothache.