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Preparation method and application of Sargassum henslowianum fucosan sulfate

A technology of Sargassum fucoidan sulfate and Sargassum Heinzii, which is applied in the direction of antiviral agents, etc., can solve the problems such as the material basis of drug effect and the mechanism of action are not clear, and achieve the effect of novel mechanism of action and definite curative effect

Active Publication Date: 2020-10-09
TIANJIN MEDICAL UNIV
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Problems solved by technology

Although some scholars have carried out chemical research on the polysaccharide components of Sargassum heinzii in recent years, due to the complexity of the polysaccharide structure and the limitation of analysis methods, the research on the polysaccharides of Sargassum heinzii is limited to the extraction process, The research on the composition of monosaccharides, the pharmacological activity is therefore limited to the research level of crude polysaccharides, and its pharmacodynamic material basis and mechanism of action are still unclear

Method used

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  • Preparation method and application of Sargassum henslowianum fucosan sulfate
  • Preparation method and application of Sargassum henslowianum fucosan sulfate
  • Preparation method and application of Sargassum henslowianum fucosan sulfate

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preparation example Construction

[0052] Prepare the preparation method of Heinz Sargassum fucoidan sulfate ester, the specific steps are as follows:

[0053] Step 1: Take the dried Sargassum henley medicinal material, reflux the hot water, concentrate the obtained solution or obtain an extract;

[0054] Step 2: adding ethanol to the extract for alcohol precipitation, collecting the alcohol precipitate and redissolving it in water, dialysis with deionized water, and lyophilizing the dialyzed internal solution to obtain the crude polysaccharide SHCP from Sargassum heinzii;

[0055] Step 3: Take Heinz Sargassum crude polysaccharide SHCP to make a crude polysaccharide solution, and obtain Heinz Sargassum fucoidan sulfate SHAP through multiple column chromatography purification;

[0056] Wherein, the specific purification steps in step three are as follows:

[0057] Step A prepares the crude polysaccharide SHCP solution of Sargassum Heinzii, passes through an anion exchange column, and uses H 2 O, 0.5mol / L NaCl,...

Embodiment 1

[0071] Example 1 Extraction, separation and purification of Heinz Sargassum fucoidan sulfate

[0072] The extraction and separation flow chart of Heinz Sargassum Fucoidan Sulfate is as follows figure 1 shown. Take the dried medicinal material of Sargassum Heinz, add 3 times the volume of absolute ethanol and degrease at room temperature for 24 hours, filter the dregs to remove the alcohol smell, add 30 times the quality of water, extract 3 times with hot water reflux, 2 hours each time, combine three times The filtrate was concentrated under reduced pressure. Add 98% ethanol to the concentrated solution to a final concentration of 80%. Depending on the situation, 95% ethanol or absolute ethanol can also be used, and put it at 4°C overnight for alcohol precipitation. The alcohol precipitate was redissolved in deionized water, dialyzed against deionized water with a 12000Da molecular weight dialysis bag, and the solution in the bag was collected, concentrated under reduced pre...

Embodiment 2

[0076] Example 2 Heinz Sargassum Fucoidan Sulphate SHAP-1 and SHAP-2 Structural Characteristic Analysis

[0077] 1. Homogeneity analysis and molecular weight determination

[0078] Take 5mg of pullulan series standard substances (molecular weights are 642, 337, 194, 107, 47.1, 21.1, 9.6, 6.1kD respectively), fully dissolve in 1 mL of ultrapure water, and prepare 5mg·mL respectively -1 Pullulan series standard solution, pass through 0.22μm filter membrane, set aside. Take 10 μL of the standard solution and inject it into a high-performance gel chromatograph (HPGPC), and use 0.02M CH 3 COONH 4 Elution, the flow rate is 0.6mL·min -1 , column temperature 40°C. Take the retention time A as the ordinate, and the molecular weight C as the abscissa to draw the standard curve A=-1.449C+19.868, R 2 = 0.9968. Take 5 mg of a single component sample of Sargassum polysaccharide from Heinz, fully dissolve it in 1 mL of ultrapure water, and configure it to 5 mg·mL -1 The sample solutio...

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Abstract

The invention provides Sargassum henslowianum fucosan sulfate which is composed of fucose and galactose and which has molecular weight of 4.0*10<5> to 6.0*10<5>. The Sargassum henslowianum fucosan sulfate is extracted from Sargassum henslowianum. By extracting Sargassum henslowianum crude polysaccharide and subjecting the crude polysaccharide to multiple times of purification with h ion exchange chromatography and molecular sieve chromatographic columns, the Sargassum henslowianum fucosan sulfate capable of being used for anti-herpes virus research is obtained. According to the scheme, the natural polysaccharide which has a novel antiviral action mode, is low in toxicity, efficient, safe and easy to accept can be separated from the marine medicinal resource Sargassum henslowianum, and a research basis can be provided for further developing antiviral drugs which have novel action mechanisms, definite curative effects and definite targets. The Sargassum henslowianum fucosan sulfate can play an antiviral role by effectively inhibiting herpes viruses from being adsorbed to the surface of host cells, and the acting site of the Sargassum henslowianum fucosan sulfate is the surface of thecells, can act on the first step of virus infection and does not enter the cells, so that the possibility of causing toxic and side effects is relatively small.

Description

technical field [0001] The invention belongs to the field of antiviral drugs, in particular to a preparation method and application of Heinz Sargassum fucoidan sulfate. Background technique [0002] Herpes simplex virus type II (HSV-2) can cause infectious diseases such as genital herpes and congenital herpetic encephalitis, and is also an important factor affecting human immunodeficiency virus (Human immunodeficiency virus, HIV) infection. The current treatment methods for HSV-2 infection mainly include vaccine immunization and antiviral drug therapy. Since 1990, the six clinical trials of HSV-2 vaccine development have all ended in failure. There are still many problems to be solved in HSV-2 vaccine research Further solving, it may take a long time to finally come out, therefore, drug therapy is still the main means for the treatment of HSV-2 infection at present. [0003] Due to the late start of the development of antiviral drugs, currently clinically effective drugs fo...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/22
CPCC08B37/006C08B37/0003A61P31/22
Inventor 董彩霞李轶邓菲
Owner TIANJIN MEDICAL UNIV
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