40results about How to "Increase fermentation intensity" patented technology

The invention provides a method for preparing citric acid by fermentation. The method comprises the following steps of: under the condition that the citric acid can be generated, inoculating aspergillus niger to a fermentation medium for fermentation, wherein the citric acid generated during fermentation is separated, and the concentration of the citric acid in fermentation liquor is enabled to be not higher than 13.5% by weight during fermentation and at a fermentation terminal point. According to the method, preferably, the citric acid generated during fermentation is separated by means of adopting an anion exchange adsorption method, so that the yield, fermentation strength and fermentation conversion rate of the citric acid are effectively increased, and the fermentation period is shortened simultaneously.

The invention relates to a method for producing citric acid by using a high-strength fermentation technology. The method is characterized by comprising the steps as follows: using corns as raw materials; pulverizing and adding water for size mixing; continuously injecting and liquefying twice after preprocessing by a homogenizer or a colloid mill; adding appropriate nitrogen sources and acid proteinase for fermentation at the same time; and adjusting the corresponding production parameters for adaptation. The method not only improves the fermentation acid yield rate, shortens the fermentation period, improves the utilization rate of equipment, improves the economic benefit of enterprises, and promotes the competitiveness of enterprises.

The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6> / mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference forpreparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.

The invention provides a method for producing L-lactic acid through continuous fermentation. The method adopts the crop waste saccharification liquid as a carbon source and produces L-lactic acid through continuous fermentation with lactobacillus, performs primary inoculation and proliferation and repeatedly utilizes thalli for multiple times, reduces the workload in strain preparation, simplifies the fermentation process and reduces the labor intensity; and moreover, the strain is subjected to continuous fermentation acclimation, the fermentation period of L-lactic acid is shortened from 50-55 hours to 24-30 hours, the fermentation intensity of L-lactic is obviously improved, and the production efficiency is improved by over 50%. The method provided by the invention has a simple process and strong continuity, is easily applied to industrial production and can obviously reduce the production cost of L-lactic acid.

The invention discloses a method and equipment for separating volatile organic matters from a fermented product. In the method, in-situ separation of the volatile organic matters is realized by coupling fermentation with a gas stripping / vapor permeation process. The method and the equipment are characterized in that: inert gas is introduced from a gas distributor at the bottom of a fermentation tank; in-situ gas is extracted from the volatile organic matters and enters a vapor permeation device so as to separate water from the organic matters; dehydrated mixed gas enters a condenser to recycle the volatile organic matters; and non-condensable gas and a water vapor component containing a small number of organic matters can be recycled. The method and the equipment have the advantages of eliminating product inhibition, enhancing fermentation intensity, saving water and reducing energy consumption along with high in-situ movement efficiency of the volatile organic matters in a fermentation process. The volatile organic matters in the invention comprise ethanol, propanol, acetone, butanol and the like.

The invention discloses a cell cultivation method of immobilized rhizopus oryzae without a carrier. The steps are as that: (1) spores are inoculated with high concentration and then thick spores-suspended emulsion is formed. (2). a number of bacterium balls are produced by controlling the aeration condition. The proportion of sodium ion and potassium ion in the culture medium is controlled to leadthe rhizopus oryzae spawn to increase and keep the stability of the cell form. Methyl silicone oil is added to form a plurality of bacterium ball cells with certain diameter. The bacterium balls cutflow and the immobilized rhizopus oryzae without a carrier are obtained. (3) when the bacterium ball obtained is transferred to a big pot, the upgrade of the bacterium balls and the increasing of thenumber are controlled. The invention has the advantages that firstly, the appearance of bacterium ball formed in the suspending culture system is regular and the size is equal; secondly, the conditionof bacterium ball accumulated by cells is mild and controllable; thirdly, the density of bacterium ball cells is more than 104 / L and the cell activity is more than 95 percent; fourthly, the large-scaled culture is easy to realize; fifthly, the cost of immobilized carrier can be saved.

The invention provides a critic acid production fermentation technology, which is characterized in that: during a critic acid fermentation process, protease is added into a fermentation tank through a fed-batch method so as to hydrolyze the protein in a culture medium into nitrogen sources, which can be rapidly utilized by bacterial strains, such as small molecular peptides, amino acid, and the like. The method can increases the content of amino acid, which can be directly utilized by bacterial strains, in a fermentation fluid; also can fully dissolves out the starch particles, which combine with the protein, reduces the viscosity of the fermentation fluid, improves the oxygen transmission efficiency in the fermentation fluid, promotes the bacterial strains to produce acid, solves the problem that in the current critic acid production process the protein utilization effect is bad in the early fermentation period, shortens the fermentation period, reduces the residual total sugar and grain consumption for fermentation, and increases the economic profits for entrepreneurs.

The invention discloses a method for seed culture of Aspergillus niger and preparing citric acid. The method comprises the following steps: (1) Aspergillus niger mouldy bran spores which are cultivated and maturated, in order to obtain dispersed spore suspension; (2) dispersed spore suspension in the step (1) is cultivated in order to obtain germinated spores; (3) germinated spores are dispersed again, the spores are cultivated in a seed tank, in order to obtain matured seed liquid; (5) matured seed liquid is converted to a fermentation tank for cultivation and fermentation, in order to obtaincitric acid fermentation broth. The method solves the aggregation problems in spore germination process, in order to improve conglomeration rate of spores, increase quantity of mycelium pellets, effectively control uniformity of size of mycelium pellets, improve conversion efficiency of thallus, improve conversion rate of fermentation, and shorten fermentation period.