A kind of method of feeding protease in citric acid fermentation process

Abstract

The invention provides a critic acid production fermentation technology, which is characterized in that: during a critic acid fermentation process, protease is added into a fermentation tank through a fed-batch method so as to hydrolyze the protein in a culture medium into nitrogen sources, which can be rapidly utilized by bacterial strains, such as small molecular peptides, amino acid, and the like. The method can increases the content of amino acid, which can be directly utilized by bacterial strains, in a fermentation fluid; also can fully dissolves out the starch particles, which combine with the protein, reduces the viscosity of the fermentation fluid, improves the oxygen transmission efficiency in the fermentation fluid, promotes the bacterial strains to produce acid, solves the problem that in the current critic acid production process the protein utilization effect is bad in the early fermentation period, shortens the fermentation period, reduces the residual total sugar and grain consumption for fermentation, and increases the economic profits for entrepreneurs.

Description

technical field [0001] The invention relates to a method for producing citric acid, in particular to a method for adding protease in a citric acid fermentation process. Background technique [0002] Citric acid, scientific name 2-hydroxypropane-1,2,3-tricarboxylic acid, molecular formula C 6 h 8 o 7 (anhydrous), is an organic acid widely used in food, medicine and chemical industry. With the development of the economy, the demand for citric acid in all walks of life will rise steadily. Of course, the challenges and opportunities faced by various enterprises are also increasing. It is mainly used in food industry, pharmaceutical industry and chemical industry, and it is also widely used in industrial fields such as electronics, textiles, petroleum, leather, construction, photography, plastics, casting and ceramics. [0003] At present, in the production of citric acid fermentation, there are problems in the early stage of fermentation, such as poor protein utilization by ...

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Problems solved by technology

[0004] In view of the problems referred to above, the present invention proposes a method of adding protease in the citric acid fermentation process, which improves the acid production rate of fermentation, shortens the fermentation period, and can improve acid production and conversion rate, and solves the problem of fermentation in the current production of citric acid. Insufficiency of the poor protein utilization effect of the initial strains, especially the lack of amino acids that can be quickly utilized by the strains, resulting in slow growth of the strains, delayed fermentation of acid production, long fermentation cycle, high energy consumption and low utilization of equipment, etc.