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Method for preparating citric acid by fermentation

A technology of citric acid and fermentation broth, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of shortened fermentation period, low fermentation conversion rate, low fermentation intensity and the like

Active Publication Date: 2015-04-22
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of low citric acid production, low fermentation intensity, low fermentation conversion rate and long fermentation period of the citric acid produced by the prior art, and provide a method for preparing citric acid by fermentation, so as to improve the production of citric acid, ferment Intensity, fermentation conversion and shortening the fermentation period

Method used

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  • Method for preparating citric acid by fermentation

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0066] Preparation of fermentation medium

[0067] 1) Crushing of raw materials: The harvested corn is moistened and stewed in a hot water tank until the water content of the corn is 15% by weight, and then crushed by a grinder (Jiangsu Muyang Group Co., Ltd., 968-3 type) to obtain crushed starchy raw materials Products (50% by weight of crushed particles have a particle diameter of less than 0.1 cm);

[0068] 2) Sizing: mixing water and starchy raw materials to obtain starch slurry, the weight ratio of the starchy raw material to water is 7:3, and the pH value of the starch slurry is 5.8.

[0069] 3) Enzymolysis: mix the starch slurry obtained in 2) with amylase (Novozymes, α-amylase, all of which are amylases in the examples of the present invention), and carry out at 90°C and pH 5.5 Enzymolysis was carried out for 100 minutes, and the amount of amylase used was 40 enzyme activity units per gram of the pulverized product to obtain an enzymolysis product.

[0070] 4) Filtra...

preparation example 2

[0075] Preparation of fermented strain seed solution

[0076] Dilute part of the enzymatic hydrolysis product in step 3) of Preparation Example 1 with water until the sugar content is 10% (weight ratio) to obtain a culture solution, put the culture solution into a seed tank, heat to 121°C for disinfection, maintain it for 30 minutes, and then quickly cool down To 37 DEG C, insert Aspergillus niger bacterial classification (Aspergillus niger T01, Tianjin Institute of Industrial Microbiology, be this Aspergillus niger bacterial classification in the embodiment of the present invention, inoculum size is: every gram of culture fluid is a benchmark, inoculates 3 * 10 5 spores), cultured at pH 6, temperature 36°C, and 0.4V / (V·min) aeration conditions; the growth of Aspergillus niger was observed by sampling microscope inspection, acidity measurement and pH measurement. After 27 hours, when the pH is 2.0, the acidity is 10g / L, the size of the bacteria balls is uniform, and the hyphae...

Embodiment 1

[0078] This example is used to illustrate the method for preparing citric acid by fermentation provided by the present invention.

[0079] The Aspergillus niger seed liquid cultivated in Preparation Example 2 was added to the fermentation medium 1 in Preparation Example 1 to start fermentation, wherein, in terms of per gram of fermentation medium, the inoculum size of Aspergillus niger was 3.0 × 10 4 spores, the fermentation conditions include: the temperature is 37°C, the pressure is 0.05Mpa, the ventilation rate is 0.2 volume: (volume·min), and the stirring speed is 80rpm. When the concentration of citric acid in the fermented liquid reaches 4% by weight, start to start the resin column that is filled with SQD816 anion exchange resin (resin filling capacity is 70% of resin column volume; The ratio of the volume of the volume of anion exchange resin and the volume of citric acid solution is 1:10), that is, firstly open the valves at the upper inlet and lower outlet of the res...

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Abstract

The invention provides a method for preparing citric acid by fermentation. The method comprises the following steps of: under the condition that the citric acid can be generated, inoculating aspergillus niger to a fermentation medium for fermentation, wherein the citric acid generated during fermentation is separated, and the concentration of the citric acid in fermentation liquor is enabled to be not higher than 13.5% by weight during fermentation and at a fermentation terminal point. According to the method, preferably, the citric acid generated during fermentation is separated by means of adopting an anion exchange adsorption method, so that the yield, fermentation strength and fermentation conversion rate of the citric acid are effectively increased, and the fermentation period is shortened simultaneously.

Description

technical field [0001] The invention relates to a method for fermenting and preparing citric acid. Background technique [0002] Citric acid is the largest acid among organic acids. Due to its excellent physical and chemical properties, it is widely used in medicine, chemistry, electronics, textiles, petroleum, leather, construction, photography, plastics, casting and ceramics and other industrial fields. [0003] At present, the fermentation technology adopted by the citric acid fermentation industry is usually an intermittent liquid submerged secondary fermentation technology that feeds once and discharges once. The fermentation process is as follows: Enzymatic hydrolysis of starchy raw materials to prepare seed medium and fermentation medium; insert Aspergillus niger into the seed tank, expand the culture and then inoculate it into the fermenter for fermentation to produce citric acid. After the fermentation stops, the fermentation liquid The bacterial residue and fermen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C07C59/265C07C51/47C12R1/685
Inventor 罗虎卢宗梅杨儒文鲁小云
Owner COFCO BIOTECHNOLOGY CO LTD
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