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Production process of fructo-oligosaccharide-neokestose

A new cos sugar and cos sugar technology, applied in the field of bioengineering, can solve the problems of no patent reports on new cos sugar, and achieve the effect of high activity of embedded live bacteria, avoiding direct mixing, and convenient separation of products

Inactive Publication Date: 2015-12-02
董晓娜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among the various immobilization technologies, the adsorption and embedding of natural polymers and synthetic polymer materials is the most active method of microbial cell immobilization. However, there is no patent report on the use of immobilized cells to prepare neocos sugar.

Method used

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  • Production process of fructo-oligosaccharide-neokestose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of bacteria:

[0037] Pick a ring of bacteria from the slant and place it in a 250mL shaker flask containing 25mL medium, culture it on a shaking table at 18°C ​​and 180r / min for 48h to obtain the primary seed liquid; then inoculate the primary seed culture liquid into 100mL of the above culture In a 500mL Erlenmeyer flask based on the base, 18°C, 180r / min shaker culture for 48h to get the secondary seed solution; inoculate the secondary seed solution with 10% of the inoculum to the fermenter, pH6.0, culture temperature 18°C, ventilation volume: 0.6vvm, the stirring speed was 160r / min, the fermentation time was 24h, and the fermentation broth was centrifuged at 4°C and 5000rpm for 10min to obtain Phaffia yeast cells.

[0038] The composition of the seed medium is: 10g sucrose, 3g peptone, 2g yeast extract, 2g wort juice, 1000mL water, pH6.0;

[0039]The composition of the fermentation medium is: 30 g of sucrose, 3 g of peptone, 2 g of yeast extract, 2 g...

Embodiment 2

[0050] (1) Preparation of bacteria:

[0051] Pick a ring of bacteria from the slant and place it in a 250mL shaker flask containing 25mL medium, and culture it on a shaker at 22°C and 180r / min for 32h to obtain a first-grade seed solution; then inoculate the first-grade seed culture solution into 100mL of the above In the base 500mL conical flask, 22 ℃, 180r / min shaker culture 32h obtains the secondary seed liquid; Inoculate the secondary seed liquid with 12% of the inoculum to the fermenter, pH6.2, culture temperature 22 ℃, ventilation volume: 0.8vvm, the stirring speed is 200r / min, the fermentation time is 32h, and the fermentation liquid is centrifuged at 4°C and 5000rpm for 10min to obtain Phaffia yeast cells.

[0052] The composition of the seed medium is: 30g sucrose, 5g peptone, 4g yeast extract, 4g wort juice, 1000mL water, pH6.2;

[0053] The composition of the fermentation medium is: 60g of sucrose, 5g of peptone, 4g of yeast extract, 4g of wort juice, 1000mL of wat...

Embodiment 3

[0064] (1) Preparation of bacteria:

[0065] Pick a ring of bacteria from the slant and place it in a 250mL shaker flask containing 25mL medium, and culture it on a shaking table at 28°C and 180r / min for 24h to obtain a first-grade seed liquid; then inoculate the first-grade seed culture liquid into 100mL of the above In the base 500mL conical flask, 28 ℃, 180r / min shaker culture 24h obtains the secondary seed liquid; Inoculate the secondary seed liquid with 15% of the inoculum to the fermenter, pH6.5, culture temperature 28 ℃, ventilation volume: 1.0vvm, the stirring speed is 220r / min, the fermentation time is 24h, and the fermentation liquid is centrifuged at 4°C and 5000rpm for 10min to obtain Phaffia yeast cells.

[0066] The composition of the seed medium is: 50g sucrose, 8g peptone, 8g yeast extract, 6g wort juice, 1000mL water, pH6.5;

[0067] The composition of the fermentation medium is: 100 g of sucrose, 8 g of peptone, 8 g of yeast extract, 6 g of wort juice, 1000 ...

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Abstract

The ivneniton discloses a production process of fructo-oligosaccharide-neokestose. Immobilized phaffia rhodozyma cells are used repeatedly, continuous and high-intensity production of fructo-oligosaccharide-neokestose is performed through phaffia rhodozyma cells immobilized with an embedding method, the production cycle is shortened, the yield of neokestose is increased, the production cost is reduced, industrial and large-scale production of fructo-oligosaccharide-neokestose is successful, and the production process has excellent application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a production process of fructooligosaccharide-Neocos sugar. Background technique [0002] Cane fructooligosaccharides, also known as oligofructose or fructooligosaccharides, are widely found in natural fruits and vegetables. They not only taste good, but also have excellent physiological functions. It can promote the proliferation of intestinal bifidobacteria, regulate the balance of intestinal flora, improve and regulate intestinal function, prevent constipation and diarrhea; in addition, it has low or no energy, prevents dental caries, lowers serum cholesterol, lowers blood lipids and blood pressure, etc. effect. The fructooligosaccharides usually produced include kestose, kestose, and fructopentose, all of which are linear molecules, that is, they are all 1F types, and the research on 6G type fructooligosaccharides (such as new cosose) is even more difficult. few. At p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12N11/10C12N11/08C12R1/645
CPCY02P20/50
Inventor 董晓娜
Owner 董晓娜
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