The invention belongs to the technical field of medicines, and particularly relates to selenium-rich eye protection liquid and a preparation method thereof. The eye protection liquid comprises the following components: selenium-rich green tea, cordyceps militaris, propolis, corn stigma, soybeans, field sowthistle herb, purple sweet potatoes, taurine, aloe, water-soluble lutein, and vitamin B1. Themethod comprises the following steps of: filtrate preparation, cordyceps militaris extract preparation, corn stigma extraction, mixed feed liquid preparation, distillate A preparation, finished product preparation and the like. During combination, the selenium-rich green tea, the corn stigma, the soybeans, the field sowthistle herband the purple sweet potatoes are added, have the effects of improving eyesight, relieving eye fatigue, preventing and improving myopia, and can promote the absorption of selenium and other nutrient elements, improve the vision, and quickly relieve eye fatigue. Thesoybeans, the cordyceps militaris and the purple sweet potatoes have high amino acid content, and can promote the taurine part to be integrated with other amino acids, and improve the proliferation, migration and differentiation of cells. The selenium-rich eye protection liquid prepared by the invention also has good antibacterial and anti-inflammatory capability and little irritation to eyes, canbe used for a long time, and has good application environment.