The invention provides a method for judging the time phase of embryonic development of Chinese softshell turtles without
anatomy and belongs to the field of temperature determining gender and determining animal sex differentiation and gender regulation.In the incubation season of the Chinese softshell turtles, zygotes of the Chinese softshell turtles are collected to be hatched in a naked mode; in the whole naked
hatching process, the change situation of fertilization spots is observed, and the change situation of blood streaks is observed through illumination, photographed and recorded.During the first
hatching week, the time phase changes fast, embryos are too small and can not be observed with the naked eyes to achieve accurate judgment, accordingly, during the first
hatching week,
embryo anatomical observation is not carried out, starting from the eighth day of hatching, 4-6 zygotes are taken each day, appearance of the zygotes is observed and then dissected, the embryonic development situation is observed through a stereoscopic
microscope, text description, photographing and recording are carried out, and by referring to the division standard of the time phase of embryonic development of the turtles, the time phase of the embryonic development of the Chinese softshell turtles is divided.Without dissecting the zygotes, the accurate development time phase of the whole process from the time before beginning of the
temperature sensitive period of the Chinese softshell turtles to finishing of the embryonic development can be obtained by observing the appearance of the zygotes.