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Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy

A technology for embryonic development and Chinese soft-shelled turtles, applied in fish farming, application, climate change adaptation and other directions, can solve the problem of equality of embryonic development without seeing turtles and soft-shelled turtles, and achieve the effect of reducing the workload of judgment.

Inactive Publication Date: 2016-07-06
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on judging the development phase of turtle embryos by their appearance

Method used

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  • Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy
  • Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy
  • Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: During the spawning season of Chinese soft-shelled turtle (May to July), 150 fertilized eggs of Chinese soft-shelled turtle were collected for hatching, and the hatching method was naked hatching. The incubation device includes a centrifuge tube and a dissection plate. The fertilized eggs are placed flat on the centrifuge tube rack with the animal pole upward. The distance between the fertilized eggs is kept at about 1.2cm. The centrifuge tube rack is placed on the dissection plate, and a little tap water (water depth 2~ 3cm), place the incubation device in a temperature-controlled and humidity-controlled incubation chamber, the relative humidity of the incubation chamber is maintained between 65% and 88%, and the incubation temperature is controlled at 29±0.1°C. In the early stage of incubation, the relative humidity of the incubation room is controlled at 65%, the relative humidity in the middle incubation period is increased to 75%, and the relative humidit...

Embodiment 2

[0078] Example 2: During the spawning season of Chinese soft-shelled turtle (May to July), 150 fertilized eggs of Chinese soft-shelled turtle were collected for hatching, and the hatching method was naked hatching. The incubation device includes a centrifuge tube and a dissection plate. The fertilized eggs are placed flat on the centrifuge tube rack with the animal pole upward. The distance between the fertilized eggs is kept at about 1.2cm. The centrifuge tube rack is placed on the dissection plate, and a little tap water (water depth 2~ 3cm), place the incubation device in a temperature-controlled and humidity-controlled incubation chamber, the relative humidity of the incubation chamber is maintained between 65% and 88%, and the incubation temperature is controlled at 31±0.1°C. In the early stage of incubation, the relative humidity of the incubation room is controlled at 65%, the relative humidity in the middle incubation period is increased to 75%, and the relative humidit...

Embodiment 3

[0080] Example 3: During the spawning season of Chinese soft-shelled turtle (May to July), 150 fertilized eggs of Chinese soft-shelled turtle were collected for hatching, and the hatching method was naked hatching. The incubation device includes a centrifuge tube and a dissection plate. The fertilized eggs are placed flat on the centrifuge tube rack with the animal pole upward. The distance between the fertilized eggs is kept at about 1.2cm. The centrifuge tube rack is placed on the dissection plate, and a little tap water (water depth 2~ 3 cm), place the incubation device in a temperature-controlled and humidity-controlled incubation chamber, the relative humidity of the incubation chamber is maintained between 65% and 88%, and the incubation temperature is controlled at 32±0.1°C. In the early stage of incubation, the relative humidity of the incubation room is controlled at 65%, the relative humidity in the middle incubation period is increased to 75%, and the relative humidi...

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Abstract

The invention provides a method for judging the time phase of embryonic development of Chinese softshell turtles without anatomy and belongs to the field of temperature determining gender and determining animal sex differentiation and gender regulation.In the incubation season of the Chinese softshell turtles, zygotes of the Chinese softshell turtles are collected to be hatched in a naked mode; in the whole naked hatching process, the change situation of fertilization spots is observed, and the change situation of blood streaks is observed through illumination, photographed and recorded.During the first hatching week, the time phase changes fast, embryos are too small and can not be observed with the naked eyes to achieve accurate judgment, accordingly, during the first hatching week, embryo anatomical observation is not carried out, starting from the eighth day of hatching, 4-6 zygotes are taken each day, appearance of the zygotes is observed and then dissected, the embryonic development situation is observed through a stereoscopic microscope, text description, photographing and recording are carried out, and by referring to the division standard of the time phase of embryonic development of the turtles, the time phase of the embryonic development of the Chinese softshell turtles is divided.Without dissecting the zygotes, the accurate development time phase of the whole process from the time before beginning of the temperature sensitive period of the Chinese softshell turtles to finishing of the embryonic development can be obtained by observing the appearance of the zygotes.

Description

technical field [0001] The invention belongs to the field of sex differentiation and sex regulation of temperature-determined sex-determining animals, and in particular relates to a method for judging the development phase of soft-shelled turtle embryos without dissection. Background technique [0002] Chinese soft-shelled turtle (Trionyx sinensis) belongs to Reptilia, Testudinata, Trionychidae, and Pelodiscus. It is a famous and precious edible aquatic animal in my country. Very popular. The Chinese soft-shelled turtle is a temperature-sex-determined animal. Its sex depends on the incubation temperature during a certain period of incubation (temperature-sensitive period). During this period, high-temperature incubation will produce more male turtle seedlings, and vice versa. . Because the male soft-shelled turtle grows faster than the female soft-shelled turtle, and its food value is also higher than that of the female soft-shelled turtle, the qualitative breeding technolo...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K61/00
CPCY02A40/81A01K67/02A01K61/00
Inventor 雷思佳任琴秦艳卿叶世洲
Owner SHENZHEN POLYTECHNIC
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