The invention relates to the technical field of automatic assembling, in particular to automatic assembling equipment of needles with spring holders for acupuncture and moxibustion. The automatic assembling equipment comprises a vibration feeding mechanism, a jet feeding mechanism, an indexing mechanism, a first linear conveying mechanism, a feeding robot, a discharging robot, an inserting mechanism, a second linear transportation mechanism, a rack and a controller, wherein the output end of the vibration feeding mechanism is sequentially connected with the input end of the jet feeding mechanism, the output end of the jet feeding mechanism is sequentially connected with the input end of the indexing mechanism, the first linear transportation mechanism is arranged on one side of the indexing mechanism, the feeding robot, the discharging robot and the inserting mechanism are all fixedly connected with the rack and are all distributed around the indexing mechanism, and the structures of the horizontal moving parts and vertical moving parts of the feeding robot and the discharging robot are identical. According to the scheme, the manpower is saved, the assembling efficiency is high, the economic benefit is good, positioning is accurate, and the quality of a finished product is excellent.