The invention relates to a clinical-laboratory traumatic bactericidal inflammation-diminishing drug and a preparation method thereof, the drug is prepared from effective components comprising the following raw materials: callicar pa nudiflora Hook. et Arn, magnolia officinalis, sambucus chinensis, honeysuckle, ganoderma lucidum, aloe leaf, radix angelicae pubescentis, radix zanthoxyli, purslane, dandelion, leptochloa chinensis, sweet wormwood, frankincense, astragalus, embelia laeta (L.) mez root and cortex ailanthi; the drug has the effects of dispelling wind-evil and relieving pains, clearing heat and removing toxicity, diminishing inflammation and killing bacterium, is suitable for bacterium killing and inflammation diminishing of traumatic wound specimen extraction in a clinical laboratory, and also can be used for skin and mucosa disinfection, skin trauma disinfection, and treatment of trichomonas vaginitis, colpitis mycotica, skin fungal infection and the like; the compatibility of the selected medicinal materials of the drug is appropriate, and the drug is prepared by use of a modern advanced pharmaceutical technology, is safe to use, has no stimulation and no side effects, can kill bacterium propagules, fungus, protozoon, and part of virus, and is worthy of wide use in the clinical laboratory.