The invention discloses a sterilizing and disinfecting agent used for operating rooms, and a preparation method thereof, and belongs to the field of traditional Chinese medicines. The effective components of the sterilizing and disinfecting agent comprise, by weight, 26-50 parts of
Nephelium lappaceum L., 26-50 parts of
Herb of Himalayan Foamflower, 25-49 parts of
Herb of Whitebracteole Bugle, 24-48 parts of
Herb of White Chinaure, 24-48 parts of Christmas flower, 24-48 parts of Leaf of Emblic Leafflower, 23-47 parts of garlic, 22-46 parts of Root of Chinese Bushclover, 22-46 parts of
Syzygium aromaticum, 21-45 parts of Herba Siegesbeckiae, 20-44 parts of
water lily, 20-44 parts of common
embelia root branchlet and leaf, 20-44 parts of Viola hamiltoniana and 19-42 parts of Root of Torpedograss. The sterilizing and disinfecting agent has the advantages of substantial effect, good safety in use, no
irritation, low cost, effective killing of viruses,
bacteria, fungi and pathogens, avoiding of the propagation of the pathogens in air, reduction of infection in operations, and improvement of the safety of the operations, and is very suitable for being massively promoted and used in the operating rooms of hospitals.