64results about How to "Accurate spatial structure" patented technology

The invention discloses a near blue fluorescent material and a preparation method thereof. The material is prepared through chemical reactions of quinoline carboxylic acid and 4,4'-bipyridine with conjugated extended pi bonds, and zinc ions with optical activity. The fluorescent material is a zinc quinoline carboxylic crystal with a molecular formula of C84H56N8O8Zn2. The crystal belongs to a crystal system of triclinic system, and a space group of P-1. Cell parameters of the crystal are that: alpha= 91.357 DEG, gamma=106.573 DEG, and beta=101.953DEG. The zinc ions have two coordination modes, one is a square pyramid coordination mode, wherein two nitrogen coordinating atoms come from two different 4,4'-bipyridines, and three oxygen coordinating atoms come from two different quinoline carboxylic acid ligands; the other is a octahedral coordination mode, wherein two nitrogen coordinating atoms come from two different 4,4'-bipyridines, and four oxygen coordinating atoms come from two different quinoline carboxylic acid ligands. The material is advantaged in that: the fluorescent material contains abundant optically active Zn (II) ions and conjugated extended bonds, and has good near blue light emission performance.

The invention discloses a 5-aminoorotic acid dinuclear cadmium complex. The 5-aminoorotic acid dinuclear cadmium complex is characterized in that the complex is prepared from a cadmium salt, an orotic acid derivative and 2,2'-bipyridine through a hydrothermal method; the complex has a definite space structure and an accurate molecular formula; and synthesis steps are simple. The constitutional unit of the complex comprises two metallic cadmium ions, two 5-aminoorotic acids, one oxalate ions, two 2,2'-bipyridines, two coordination water molecules and four object water molecules; the molecular formula of the complex is C32H36N10O18Cd2; the complex belongs to a triclinic system; the space group of the complex is P-1; and cell parameters are that a is equal to 8.8941 angstrom, b is equal to 9.2338 angstrom, c is equal to 13.759 angstrom, alpha is equal to 97.978 DEG, beta is equal to 97.975 DEG and gamma is equal to 116.416 DEG. The prepared complex is a purple fluorescent material, has excellent physicochemical performance and shows wide application prospects in the field of photoelectric materials or catalytic materials.

The invention discloses a method for expressing human interleukin 7 in eucaryotic host. When the eucaryotic host is pichia X-33, the method comprises the following steps of: (1) cloning an IL-7 gene of human; (2) building an eucaryotic expression vector; (3) transforming recombinant vector to the eucaryotic yeast host; and (4) expressing a rIL-7 protein in the yeast host. A prior method for expressing IL-7 by using prokaryotic host escherichia coli is changed, and a method for expressing IL-7 through eucaryotic host pichia is searched out, thereby ensuring an IL-7 biologic activity and obtaining a large amount of stable protein.

The invention discloses a cobalt complex and a preparation method thereof, and the cobalt complex is characterized by having good electrocatalytic activity on hydrogen peroxide, the molecular formula of the cobalt complex is C26H26N4O10Co, the crystal system is orthorhombic, the space group is Pbcn, the cell parameters alpha, gamma and beta satisfy: a=15.928Angstrom, b=10.602Angstrom, c=15.596Angstrom, alpha is equal to gamma, is equal to beta and is equal to 90 degrees. The cobalt complex prepared in the invention is obtained by a mixing reaction of a cobalt salt and a derivative of 5-aminosalicylic acid, namely, 5-((2-pyridyl) methylene amino)-2-hydroxybenzoic acid according to a certain proportion, the cobalt complex has a specific spatial structure and an accurate molecular formula, the synthesis steps are simple, the yield can reach 45-70%, and the cobalt complex has good electrocatalytic activity, thereby being capable of being used as an electrocatalytic active material having a potential application prospect.

The invention discloses a copper complex and a preparation method thereof, and the copper complex is characterized by having good electrocatalytic activity on hydrogen peroxide, the molecular formula of the copper complex is C46H34N6O14Cu2, the crystal system is clinorhomboidal, the space group is P-1, the cell parameters are as follows: alpha is equal to 99.90 degrees, beta is equal to 101.2 degrees and gamma is equal to 93.69 degrees. The copper complex is prepared from a mixing reaction of a copper salt, 5-(di(carboxymethyl) amino)-2-hydroxybenzoic acid and 1,10-phenanthroline according to a certain proportion, the copper complex has a specific spatial structure and an accurate molecular formula, the synthesis steps are simple, and the yield can reach 30-70%. The copper complex prepared in the invention has good electrocatalytic activity, thereby having a broad application prospect of being used as a potential electrocatalytic active material.

The invention discloses a compound with electrochemical response to formaldehyde and a preparation method thereof. The cobalt complex electrocatalytic material is a 4,4'-dipyridine sulfadiazine cobalt compound with certain space structure, and the molecular formula is C40H34CoN12O4S2. The preparation method of the sulfadiazine cobalt compound electrocatalytic material is implemented by carrying out chemical reaction on sulfadiazine with conjugated extended pai bond and 4,4'-dipyridine to obtain the novel cobalt compound. The compound used as an electrocatalytic active material has accurate space structure and accurate molecular formula; the cobalt ions can form stable coordinate bonds with the ligand by utilizing 3d orbit, thereby being beneficial to the transfer of electrons and promoting the proceeding of the electrochemical reaction, and thus, the compound has favorable electrocatalytic activity for formaldehyde. The cobalt complex used as the electrocatalytic active material has wide application prospects.

The invention discloses a blue-light-emitting fluorescent material and a preparation method thereof. The invention is characterized in that the fluorescent material is a cadmium compound of orotic acid, which is prepared by carrying out chemical reaction on orotic acid with carboxylic acid, phenanthroline and cadmium ions with optical activity; the molecular formula of the fluorescent material is C44H52N12O28Cd2, the crystal system is triclinic, and the space group is P-1; and the cell parameters are as follows: a=9.5220 Angstroms, b=11.8790 Angstroms, c=12.6141 Angstroms, alpha=73.082 degrees, gamma=78.073 degrees, and beta=81.100 degrees. The cadmium ions are in a pentagonal bipyramidal coordination mode, two nitrogen coordinating atoms are from the phenanthroline, three oxygen coordinating atoms are from orotic acid, and two oxygen coordinating atoms are from water. The fluorescent material has the advantages of accurate space structure and accurate molecular formula, and contains abundant Cd(II) ions with optical activity and carboxylic acid with strong coordinating capacity, thereby being beneficial to electron transition and energy transmission. The fluorescent material has favorable photoelectric activity and favorable blue light emission performance.

The invention discloses an oxalate-containing two-dimensional terbium coordination polymer green fluorescent material and a preparation method of the oxalate-containing two-dimensional terbium coordination polymer green fluorescent material and relates to a green fluorescent material and a preparation method of the green fluorescent material. The invention aims to solve the problems that the conventional organic green luminous material is low in stability and the organic green luminous material prepared by the conventional method is low in yield. The oxalate-containing two-dimensional terbium coordination polymer green fluorescent material has the molecular formula of C9H12NO9Tb. The preparation method comprises the following steps of: 1, stirring and mixing; 2, heating; and 3, washing and drying, and thus obtaining the oxalate-containing two-dimensional terbium coordination polymer green fluorescent material. The oxalate-containing two-dimensional terbium coordination polymer green fluorescent material and the preparation method fo the oxalate-containing two-dimensional terbium coordination polymer green fluorescent material have the advantages that 1, the prepared oxalate-containing two-dimensional terbium coordination polymer green fluorescent material is bright in color and high in stability; 2, the method is simple and is easy to operate, the yield is up to 84-87 percent, and compared with the yield of 60-70 percent in the prior art, the yield is improved by over 14 percent. The method can be used for preparing the oxalate-containing two-dimensional terbium coordination polymer green fluorescent material.

The invention discloses a method for efficiently preparing recombinant active scorpion insect neurotoxin LqhIT2. The method mainly comprises a step (1) of optimizing and synthesizing LqhIT2 genes with C- ends provided with 6*His labels according to amino acid sequences of mature LqhIT2 toxins and characteristics of a pichia pastoris expression system and constructing pichia pastoris secretory expression vectors; a step (2) of converting recombinant vectors into a pichia pastoris host, and through screening, obtaining yeast transformants in high-level secretory expression; a step (3) of expressing recombinant scorpion insect neurotoxin LqhIT2 proteins in the pichia pastoris host, and quickly obtaining the recombinant scorpion insect neurotoxin LqhIT2 proteins with the purity over 98% through a Ni2+ affinity chromatography method, wherein the purified recombinant proteins have very strong poisonous activity on insect cells. The method is modified based on conventional expression of insect neurotoxin LqhIT2 through prokaryotic host escherichia coli, the method of using eukaryotic host pichia pastoris to express the insect neurotoxin LqhIT2 is explored, and the yeast transformants in high-level secretory expression are screened.

The invention discloses a cobalt complex with electrochemical catalytic activity and a preparation method thereof. The cobalt chemical combined electro-catalysis material is a cobalt complex with a certain spatial structure, the molecular formula of the cobalt complex is C3OH26CoN10O4S2. The preparation method of the cobalt complex chemical combined electro-catalysis material comprises the following steps: enabling sulfadiazine with conjugate big Phi bond, 2, 2'-dipyridyl and cobalt ions with electro-catalysis activity to be subjected to chemical reaction, so as to obtain the novel cobalt complex containing sulfadiazine and dipyridyl. According to the invention, the cobalt complex has electro-catalysis activity, accurate spatial structure and molecular formula; cobalt atoms can form a stable coordination bond with a ligand by using the empty d electronic orbit of the atoms, so as to facilitate electron to be transmitted, and further facilitate the proceeding of the electrochemical reaction; the prepared cobalt complex has good electro-catalysis activity, and has wide application prospects as an electro-catalysis active material.

The invention discloses a fluorescent material with a purple luminescence property and a preparation method thereof. The fluorescent material is characterized in that the fluorescent material is a zinc ethylcarbonate crystal prepared by carrying out a chemical reaction on phenanthroline and quinolinecarboxylic acid having a carboxylic group as well as zinc ions having optical activities, wherein the molecular formula of the zinc ethylcarbonate crystal is C88H64N8O12Zn2, the crystal system of the crystal is monoclinic, the space group of the crystal is P21c, and the cell parameters of the crystal are as follows: alpha is equal to 90 degrees, gamma is equal to 90 degrees, and beta is equal to 97.5650 degrees; and the zinc ion is in a deformed octahedral coordination mode, two nitrogen coordination atoms are derived from phenanthroline, three oxygen coordination atoms are derived from quinoline carboxylic acid, and an oxygen coordination atom is derived from water. The fluorescent material has the advantages of correct space structure and molecular formula, and contains Zn (II) ions with rich optical activities and carboxylic acid with strong coordination capability, thereby being beneficial to electron transition and energy transfer. Thus, the fluorescent material has a good purple luminescence property.

The invention relates to a high-activity cellulose incision enzyme artificially synthesized gene. The gene is shown as a nucleotide sequence of SEQ ID NO.1 in a sequence table; or the gene is as shownin the sequence of a biological function protein with 90 or above homology with the nucleotide sequence and same coding. According to the high-activity cellulose incision enzyme artificially synthesized gene, such as the nucleotide sequence of SEQ ID NO.1 in the sequence table, a pichia pastoris constitutive expression plasmid pGAPZalpha can be used as a carrier, and high-level recombinant secretory expression of a target protein in pichia pastoris host bacteria X33 can be realized. Meanwhile, screened high-level expression converters can realize high-level serection of a recombinant proteinby using high-density fermentation of an inorganic salt, and novel high-activity recombinant cellulose incision enzyme protein sterling can be obtained through simple nickel affinity chromatography purification. The recombinant protein has the high ability of hydrolyzing cellulose substances and generating glucose, and has important application value in aspects such as biological energy sources, fodder and food industries.

The invention discloses a fluorescent material with purple luminescence property and a preparation method thereof. The fluorescent material is characterized in that barbituric acid with relatively strong complexing capability serving as an organic ligand and zinc as a transition metal are prepared into a super-molecular zinc barbiturate compound by a solution method, the super-molecular zinc barbiturate compound has the molecular formula represented by C8H10ZnN4O8, the crystal system is an orthorhombic system, the space group is Iba2, and the cell parameters are shown in the specification, wherein alpha is equal to 90 degrees, gamma is equal to 90 degrees and beta is equal to 90 degrees. The fluorescent material has the advantages of accurate spatial structure, precise molecular formula, good thermal stability property and purple luminescence property and the fluorescent material containing a heterocyclic ring and cyclocarbonyl can be bonded with a transition metal to form a firm crystal framework by virtue of coordination bonds.

The present invention relates to an artificial synthesis gene for coding cellulose excision enzymes. The gene at least contains a DNA piece with one of the following nucleotide sequences: 1) a nucleotide sequence of SEQ ID NO.1 in a sequence table; and 2) a nucleotide sequence which has more than or equal to 90% of homology with the nucleotide sequence shown in SEQ ID NO. 1 and codes a protein with a same biological function. According to the gene sequence, a recombinant vector is further constructed to transform yeasts, so that secretory expression of a recombinant cellulose excision enzyme under induction of methanol can be realized, the active recombinant cellulose excision enzyme protein with a purity higher than 95% can be obtained through nickel affinity purification, and the activeprotein has capacity of hydrolyzing cellulose and generating glucose.

The invention discloses a preparation method for a spider neurotoxin TX4(6-1) recombinant protein. The preparation method comprises the following steps: (1) according to the amino acid sequence of TX4(6-1) mature toxin and the feature of a pichia pastoris expression system, optimizing and synthesizing gene of TX4(61-) of which the C-end is provided with a 6XHis label and constructing a pichia pastoris secretory expression carrier; (2) converting a recombinant carrier to a pichia pastoris host, screening to obtain a yeast converter of high-level secretory expression; and (3) expressing a recombinant spider insect neurotoxin TX4(6-1) protein in the yeast host, to obtain the recombinant spider insect neurotoxin TX4(6-1) protein of which the purity is more than 95% rapidly through a nickel affinity chromatography method, wherein the protein has the remarkable toxicity to silkworms. According to the preparation method, a large number of the stable recombinant spider insect neurotoxin TX4(6-1) proteins can be rapidly obtained. The preparation method has important value for the insect resistant field, specifically for cultivating insect resistant plant varieties, and the preparation method has important significance for improving the yield of crops.

The invention discloses a preparation method of a scorpion neurotoxin Bj alpha IT gene and a recombinant protein thereof. The preparation method comprises the following steps that: (1) According to the amino acid sequence of the Bj alpha IT mature toxin and the characteristics of Pichia pastoris expression systems, Bj alpha IT genes with 6*His tags at C- ends are optimally synthesized and Pichia pastoris secretion expression vectors are constructed; (2) the recombinant vectors are transferred into Pichia pastoris hosts, yeast transformants with high level secretion expression are obtained, and recombinant scorpion insect Bj alpha IT proteins with a purity of over 95% are obtained by nickel affinity chromatography. A method for expressing insect neurotoxin Bj alpha IT genes with eukaryotic host Pichia pastoris is disclosed. The recombinant scorpion insect Bj alpha IT protein with high purity is quickly obtained by the nickel affinity chromatography, and the protein performs high bioactivity at both cellular and animal levels. The protein is of great value in the field of insect resistance, especially for breeding varieties of insect resistant plants, and has important significance for improving the yield of crops.

The invention relates to recombinant pectinase, a gene thereof, a recombinant vector, a preparation method and application. The gene at least comprises DNA fragment of one of a nucleotide sequence ofSEQ ID NO.1 in a sequence table, a nucleotide sequence having higher than 90% homology with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein and a nucleotide sequence hybridized with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein. The recombinant vector is further built according to the gene sequence, yeast is transformed, in this way, constitutive secretory expression of the recombinant pectinase in a high-density fermentation condition can be realized; active protein higher than 95% in purity and with high protein concentration can be obtained through simple nickel affinity purification; the active protein has high pectin hydrolyzing activity at low temperature, and a novel and efficient enzymeproduct can be provided for increasing juice yield of fruit juice.

The invention discloses a cobalt complex electrochemically responding to hydrogen peroxide and a preparation method thereof. The cobalt complex has a very good electro-catalytic activity and a good electrochemical responding performance on hydrogen peroxide. The chemical formula of the cobalt complex is CoC5H9N3O10, the structure unit is [Co(C5HN3O6(H2O)4], the crystal system is an orthorhombic crystal system, the space group is Pca2(1), the cell parameters are as follows: a=19.0262 angstrom, b=5.4287 angstrom, c=10.4010 angstrom, and alpha=beta=gamma=90 degrees; and the cobalt ions have a hexa-coordinated octahedral geometrical configuration. The cobalt complex is prepared by mixing cobalt acetate hydrate and potassium 5-nitroorotate according to a certain ratio to carry out reactions. The preparation method has the advantages of simple operation and low cost, and is suitable for massive production. The prepared cobalt complex has a definite space structure and an accurate molecular formula, has a very good electro-catalytic activity on hydrogen peroxide, and can be advantageously used in electro-catalytic responding or electrochemical sensor detection.

The invention discloses a copper complex and a preparation method thereof, and the copper complex is characterized by having good electrocatalytic activity on hydrogen peroxide, the molecular formula of the copper complex is C46H34N6O14Cu2, the crystal system is clinorhomboidal, the space group is P-1, the cell parameters are as follows: alpha is equal to 99.90 degrees, beta is equal to 101.2 degrees and gamma is equal to 93.69 degrees. The copper complex is prepared from a mixing reaction of a copper salt, 5-(di(carboxymethyl) amino)-2-hydroxybenzoic acid and 1,10-phenanthroline according to a certain proportion, the copper complex has a specific spatial structure and an accurate molecular formula, the synthesis steps are simple, and the yield can reach 30-70%. The copper complex prepared in the invention has good electrocatalytic activity, thereby having a broad application prospect of being used as a potential electrocatalytic active material.

The invention relates to an artificially modified HtA protein and a coding gene and application thereof. The protein provided is a ribosomal toxin protein molecule that is shown in a sequence table 1,undergoes molecular modification and originates from Hirsutella thompsonii. The gene corresponding to the protein is any one DNA molecule as shown in (1)-(3): (1) a DNA molecule shown in sequence 2 in the sequence table; (2) a DNA molecule of protein that is hybridized with a DNA sequence defined by (1) in a strict condition, and encoded and has insecticidal activity; and (3) a DNA molecule of protein that has at least 95%, at least 96%, at least 97%, at least 98% or at least 99% of homology to the DNA sequence defined in (1), is encoded and has insecticidal activity. The DNA sequence of theinsect-killing protein is optimized. A method capable of highly expressing and purifying HtA recombined protein is provided, has high value for the insect resisting field, particularly cultivation ofinsect-resistant plant varieties, and has great significance for increase of crop yield.

The invention relates to an artificially synthetic gene for coding poria cocos cellulose endonuclease. The gene at least contains a DNA segment of one of the following nucleotide sequence: 1) a nucleotide sequence in SEQ ID NO.1 in a sequence table; and 2) a nucleotide sequence with 90% homology or above and coding the same biological functional protein with the nucleotide sequence in SEQ ID NO.1.Reconstruction of recombinant vectors and yeast conversion are further performed according to the gene sequence, secretary expression of the recombinant poria cocos cellulose endonuclease under induction of methanol can be realized, purification is performed by ammonium sulfate precipitation and a DEAE ion exchange column, the recombinant poria cocos cellulose endonuclease with purity higher than95% can be obtained, the enzyme has very high capacity for degrading cellulose components and producing glucose, and recombinase with high activity can be provided for cellulose decomposition and application to production of feed, textile, food and biological energy sources.

The invention relates to an experimental device and for detecting distribution status of coal fissures around drill holes and an experimental method. The experimental device comprises a pressurizing system, a pressure transfer shaft, a cylinder, a round disk, a coal sample, a chassis, a drilling rig, a grouting pump, a glue pipe, a glue body and a hollow round disk; the method includes steps of filling high-temperature fluid glue in a coal sample drill hole, and penetrating it under a certain pressure to enter the coal fissures around drill holes; after cooling the glue body, dismounting the grouting device, pulling out a coal sample and peeling the coal powder; acquiring the glue body filled in the drill hole and the coal fissures; acquiring the space structure distribution of the coal fissures around drill holes after releasing pressure, scaling down at a certain ratio, and acquiring the space structure of coal fissures around drill holes under the pressing state. The experimental device and the method solve the technical difficulties that the coal fissures around drill holes are not intuitional and exact to observe for a long time. The method and device can be applied to directly and exactly detect the distribution status of the coal fissures around drill holes, and helpful to further explore a high-efficient drilling sealing technology.

The present invention relates to an artificially synthesized gene encoding cold tolerance lactase, the gene at least contains a DNA piece of one of the following nucleotide sequences: 1) the nucleotide sequence of SEQ ID NO.1 in the sequence table; 2) the nucleotide sequence with The nucleotide sequence shown in SEQ ID NO.1 has more than 90% homology and encodes a nucleotide sequence of the same biological function protein; or 3) hybridizes with the nucleotide sequence shown in SEQ ID NO.1 and encodes Nucleotide sequences of proteins with the same biological function. According to the gene sequence of the present invention, the recombinant vector is further constructed and transformed into yeast, which can realize the constitutive secretory expression of the recombinant lactase under high-density fermentation conditions, and can obtain a purity higher than 95% through simple nickel affinity purification. And active protein with high protein concentration, the active protein has a strong activity of hydrolyzing lactose at low temperature, and will provide efficient enzyme products for converting lactose in dairy products.

The invention relates to a ribosome toxin and an encoding gene, and an application thereof. The gene provided by the invention is any one DNA molecule of the following (1)-(3): (1) a DNA molecule shown in a sequence 1 in a sequence table; (2) a DNA molecule hybridized with the DNA sequence defined by the (1) under strict conditions and encoding a protein having the activity of poisoning and killing insects; and (3) a DNA sequence having at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology with the DNA sequence defined by the (1) and encoding a protein having the activity of poisoning and killing insects. The DNA sequence of HtA is optimized, the method for high-efficiency expression and quick purification of the HtA recombinant protein is provided, is of important value for the field of insect resistance, especially for breeding of insect-resistant plant varieties, and is of important significance for increasing the yield of crops.

The invention discloses a cadmium amrinone fluorescent material and a preparation method thereof. Small organic molecular ligand of a double-pyridine structure and cadmium ions with optical activity are coordinated, a cadmium amrinone complex with a specific space structure is prepared, the molecular formula is C20H16N6O2Cd, the crystal system is orthogonal, the space group is P212121, the cell parameters are shown in the specification, and alpha is equal to beta and gamma and is equal to 90 degrees. The fluorescent material has rich optical activity and Cd (II) ions and nitrogen heterocyclic rings of unique coordination configuration, is beneficial for electron transition and energy transmission, has unique optical activity and unique blue fluorescence emitting performance and has high application value.