31 results about "Zinc ion binding" patented technology

The invention relates to an anti-tumor polypeptide, a preparation method and anti-tumor applications thereof. A purpose of the present invention is to provide a polypeptide having an anti-tumor function and a preparation method thereof. A structure of the polypeptide comprises three histidine structures containing an endostatin N-terminal zinc ion binding site, a beta-pleated sheet structure and a serial RGD sequence. According to the invention, a chemical synthesis method for the polypeptide drug is disclosed, and a tumor proliferation inhibition effect of the polypeptide is proved.

An article of manufacture and methods of making same. In one embodiment, the article of manufacture has a plurality of zinc oxide layers substantially in parallel, wherein each zinc oxide layer has a thickness d1, and a plurality of organic molecule layers substantially in parallel, wherein each organic molecule layer has a thickness d2 and a plurality of molecules with a functional group that is bindable to zinc ions, wherein for every pair of neighboring zinc oxide layers, one of the plurality of organic molecule layers is positioned in between the pair of neighboring zinc oxide layers to allow the functional groups of the plurality of organic molecules to bind to zinc ions in the neighboring zinc oxide layers to form a lamellar hybrid structure with a geometric periodicity d1+d2, and wherein d1 and d2 satisfy the relationship of d1≦d2≦3d1.

The invention discloses nano ZnO / cellulose gel particles and a preparation method thereof. According to the nano ZnO / cellulose gel particles, cellulose gel is used as a core, and the outer layer is loaded with nano ZnO. According to the preparation method, a ZnCl2 aqueous solution is used as a solvent for cellulose and a zinc source for nano ZnO; dissolving pulp cellulose fibers are cellulose raw materials; the nano ZnO / cellulose gel particles are prepared by dissolving, co-separation, hydrothermal synthesis and freeze drying. As the ZnCl2 aqueous solution is ingeniously used as the solvent for the cellulose and the zinc source for nano ZnO, other cellulosesolvents are not needed to be added, and the preparation technology is clean and environment-friendly; furthermore, after cellulose is dissolved, hydroxyl on the molecules of the cellulose is combined with zinc ions, so that generation of nano ZnO is greatly promoted, and the hydrothermal synthesis temperature is correspondingly obviously reduced; therefore, the preparation technology has the advantage of energy conservation.

The invention discloses a hydrothermal preparation method of a nano-ZnO cellulose composite material on the basis of a NaOH-urea solution and application. The method comprises the step that in the NaOH-urea cellulose solution, the nano-ZnO cellulose composite material is prepared through in-situ compounding by adopting the hydrothermal method. According to the hydrothermal preparation method of the nano-ZnO cellulose composite material on the basis of the NaOH-urea solution, after cellulose is dissolved, hydroxyl groups on molecules of the cellulose are combined with zinc ions, and the defect that a zinc source does not easily penetrate into a carrier is overcome; in addition, the adopted NaOH-urea solvent is cheap and easy to obtain, and the hydrothermal synthesis temperature is obviously reduced. The nano-ZnO cellulose composite material contains 47.5% of ZnO and has the high removal efficiency on phenol in photodegradation wastewater. The ZnO cellulose composite material prepared through the method is cheap and environmentally friendly and has the very good practicability on phenolic wastewater treatment by serving as a photodegradation catalyst.

An article of manufacture and methods of making same. In one embodiment, the article of manufacture has a plurality of zinc oxide layers substantially in parallel, wherein each zinc oxide layer has a thickness d1, and a plurality of organic molecule layers substantially in parallel, wherein each organic molecule layer has a thickness d2 and a plurality of molecules with a functional group that is bindable to zinc ions, wherein for every pair of neighboring zinc oxide layers, one of the plurality of organic molecule layers is positioned in between the pair of neighboring zinc oxide layers to allow the functional groups of the plurality of organic molecules to bind to zinc ions in the neighboring zinc oxide layers to form a lamellar hybrid structure with a geometric periodicity d1+d2, and wherein d1 and d2 satisfy the relationship of d1≦d2≦3d1.

The invention discloses a preparation method of a micro-nano ZnO / composite cellulose strip material. The method is as follows: using a ZnCl2 aqueous solution as a moistening and swelling agent and a solvent of cellulose and a zinc source of nano ZnO, using dissolving pulp cellulosic fibers as cellulose raw materials, moistening, swelling and partially dissolving the cellulose raw materials, performing injection eutectoid and hydrothermal synthesis and then freeze-drying, so as to prepare the micro-nano ZnO / cellulose strip material which uses raw cellulosic fibers and regenerated cellulose fibers as carriers. As the ZnCl2 aqueous solution is used as the moistening and swelling agent and the solvent of cellulose and the zinc source of nano ZnO, no additional cellulose solvent is required, and the preparation process is clean and environment-friendly; in addition, after cellulose is moistened, swelled and partially dissolved, hydroxyls and zinc ions of the cellulose molecules are combined, which promotes the generation of nano ZnO, and the hydrothermal synthesis temperature is obviously reduced, therefore, the preparation process has the energy saving advantage.

The invention belongs to the field of flame retardants, and particularly relates to a ZIFs nanosheet / chitosan / diatomite hybrid. The hybrid is prepared by combining the nitrogen element of a chitosan molecule with cobalt or zinc ions in a ZIFs nanosheet and then combining an amino group with a hydroxyl group on the surface of diatomite. When the hybrid is added into a polymer, the chitosan can be used as an effective carbon source, the diatomite and the ZIFs nanosheet have a certain physical barrier effect, cobalt oxide or zinc oxide generated by thermal decomposition of the ZIFs nanosheet andacidic active sites on the surface of SiO2 can promote the formation of a compact carbon layer, and the carbon layer has an excellent barrier effect, so that the flame retardance and the smoke suppression performance of the polymer material are improved. The invention also relates to a preparation method of the hybrid. The preparation method has the advantages of low reaction requirements, easilyavailable raw materials, simple technique, and realization of no environmental pollution, greenness and environmental protection by using water as the solvent.

The invention provides a bidirectional screening system construction method for directed evolution of lead binding protein. A positive screening marker ampicillin resistance gene amp and an inverse screening marker levansucrase gene sacB are adopted to form a bidirectional screening system, and the bidirectional screening system is regulated by the lead binding protein PbrR and specific promoter Ppbr expression. Results of bidirectional screening system expression verification and expression optimization show that the screening conditions of lead ions combined with lead binding protein are 50MuM of lead ions and 100Mug / mL; the screening conditions of zinc ions and lead binding protein combined with weakened optimization pressure are 50MuM of lead ions and 10% of saccharose, and the growthof cells strongly combined with the zinc ions is inhibited due to saccharose sensitivity. The optimization condition is a pressure screening condition of directed evolution of the lead binding proteinand used for weakening the interference of non-target metal zinc ions to achieve the special directed evolution of the lead binding protein; and a new screening tool is provided for specific optimization of the metal regulator protein.

The invention discloses a preparation method and application of oyster zinc chelate peptide with high water solubility. Oyster meat is used as raw material. The method comprises the following steps of: preparing oyster freeze-dried powder, extracting oyster protein, carrying out alkaline protease enzymolysis, carrying out 3KDa ultrafiltration centrifugation, carrying out SPE column purification bytaking C18 as a filler, and carrying out vacuum freeze drying to obtain the high-water solubility oyster zinc chelate peptide. The preparation method is simple and safe. The prepared high-water solubility oyster zinc chelate peptide is a natural edible component, has higher zinc ion binding capacity and high water solubility, has good solubility, is easy to absorb, can be used as a zinc supplement, and has an application prospect of practical development.

The present invention provides apparatuses and methods for determining zinc ion concentration in a fluid sample. Some apparatuses of the invention include (i) a light source designed to emit an excitatory light at a first known wavelength that activates a fluorescent emission having a second and different wavelength by at least one selected fluorescent reporter probe that generates a different fluorescent response when bound to zinc ion; (ii) at least one sample-holding component adapted to hold a liquid sample in a pathway of the excitatory light; (iii) a photodetector for measuring fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion within the liquid sample; and (iv) electronic means for creating a data signal that correlates with fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion in the liquid sample to the free zinc ion concentration. Such apparatuses and methods can be used to determine the presence of or the risk of having prostate cancer in a subject.

The invention relates to a histone deacetylase inhibitor and preparation and an application thereof, in particular to a novel histone deacetylase inhibitor based on a beta-elemene structure and a preparation method thereof, and further relates to an intermediate for synthesizing the histone deacetylase inhibitor and a preparation method thereof. The invention also relates to an application of the histone deacetylase inhibitor in preparation of antitumor drugs, and belongs to the technical field of chemical synthesis of drugs. The zinc ion combined compound and the salt or solvate thereof are shown as a general formula (I) or (II), wherein R, X, n and i are described in the claims and the specification.

There is provided inter alia an aqueous liquid pharmaceutical formulation comprising (i) an insulin compound, (ii) ionic zinc, (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a log K with respect to zinc ion binding in the range 4.5-12.3 at 25° C., and (iv) a non-ionic surfactant which is an alkyl glycoside; and wherein the formulation is substantially free of EDTA and any other zinc binding species having a log K with respect to zinc ion binding of more than 12.3 at 25° C.

The invention discloses a method for preparing a nanometer ZnO cellulose composite based on a NaOH / urea solution by using a direct precipitation process, and application of the nanometer ZnO cellulose composite. The nanometer ZnO cellulose composite is prepared in a cellulose solution of NaOH / urea through in-situ compounding via the direct precipitation process. According to the preparation method, after dissolving of cellulose, a hydroxyl group on the molecule of cellulose bonds with zinc ions, which is beneficial for uniform distribution of ZnO in cellulose; the method is simple; the solvent, i.e., the NaOH / urea solution, is cheap and easily available; and equipment used in the invention is simple and easily practicable. The nanometer ZnO cellulose composite contains 57% of ZnO, has high removal efficiency on methylene blue in photodegradation of dye wastewater, and is cheap and environment-friendly; and when used as a photodegradatic catalyst, the nanometer ZnO cellulose composite has good practicality in treatment of methylene blue wastewater.

There is provided inter aliaan aqueous liquid pharmaceutical formulationcomprising (i) an insulincompound at a concentration of 500-1000 U / ml, (ii) ionic zinc, (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a logK with respect to zinc ion binding in the range 4.5-12.3 at 25 DEG C, and (iv) a non-ionic surfactant; and wherein the formulation is substantially free of EDTA and any other zinc binding species havinga logK with respect to zinc ion binding of more than 12.3at 25 DEG C.

The invention discloses a chelating type zinc ion fluorescent probe and a preparation method and application thereof, a hemicyanine derivative is modified to synthesize a near-infrared light fluorescent molecular probe capable of specifically reacting with zinc ions, the fluorescent probe can generate an obvious optical effect after being combined with the zinc ions, and the fluorescent probe can be used for detecting the zinc ions. The intracellular zinc ion fluorescent probe can be effectively positioned, and can be used for detecting abnormity of the intracellular zinc ions.

In particular, the present invention relates to an aqueous liquid pharmaceutical formulation comprising: (i) an insulin compound; (ii) zinc ions; (iii) a concentration of 1 mM or higher selected from the group consisting of compounds having a logK for zinc ion binding at 25°C between 4.5-4.5°C. Zinc-binding substances of substances in the range of 10; (iv) zinc-binding substances selected from substances having a logK greater than 12.3 for zinc ion binding at 25° C. at a concentration of less than about 0.3 mM; and (v) non-ionic surface active agent. Related methods, uses and pharmaceutical compositions are also provided.

The invention discloses a zinc oxide semiconductor material, a preparation method thereof, a semiconductor device and an air conditioner. The preparation method of the zinc oxide semiconductor material comprises the following steps: first preparing a zinc oxide buffer layer; secondly, growing a zinc film on the zinc oxide buffer layer, and adding a first element and a second element to the zinc oxide film, wherein the ionic radius of the first element is less than the radius of a zinc ion, and the ionic radius of the second element is greater than the radius of an oxygen ion; and finally, increasing the temperature of the zinc oxide film and then annealing the zinc oxide film. The method adds two different types of elements to the zinc oxide film so that the first element and the oxygen ions are combined to form a new lattice and introduce pressure stress, and the second element and the zinc ion are combined to form a new lattice and introduce tensile stress. Macroscopically, the tensile stress and the pressure stress inside the zinc oxide film cancel out each other to form a stable P-type doped zinc oxide semiconductor material.

The invention discloses a zinc oxide antibacterial material loaded on high polymer composite bacterial cellulose, a preparation method and application thereof. The antibacterial material is added into the fermentation medium with sodium alginate or carboxymethyl cellulose as an additive, and the bacterial cellulose is combined with sodium alginate or sodium carboxymethyl cellulose during synthesis. The fermentation product has the carboxyl group in sodium alginate or sodium carboxymethyl cellulose, and the carboxyl group is used to combine with zinc ions, and the nano-zinc oxide is loaded on the polymer composite bacterial cellulose through microwave high temperature and high pressure hydrothermal reaction. The method can effectively increase the loading capacity of the nanometer zinc oxide in the bacterial cellulose, and provide stable loading. And this method is more environmentally friendly than traditional chemical grafting, and does not require the use of highly corrosive chemicals. Polymer composite bacterial cellulose-supported zinc oxide antibacterial material Bacterial cellulose with good biocompatibility has antibacterial properties and can be applied to some body surface wounds.

The invention relates to a porcine contagious pleuropneumonia actinobacillus serum 1-type zinc ion binding protein A (znuA) deletion strain, a construction method therefor and applications thereof. The microbial colllection number is CCTCC No: M2012476. The classification and nomenclature is inobacillus pleuropneumoniae APP-1( delta znuA). The collection time is November 23th, 2012. The collection place is China Center for Type Culture Collection. The collection address is Wuhan University, Wuhan, China. The znuA gene deletion mutantion strain is compared to the parent strain to study biological characteristics and pathogenic immunogenicity. The discoveries show that the pathogenicity of the gene deletion strain is obviously weaker than that of the parent strain APP. The gene deletion strain can be used for developing gene deletion inactivated vaccines of APP, and provides effective technologies and methods for preventing and treating porcine contagious pleuropneumonia safely and effectively.

The invention discloses a hydrothermal preparation method of a nano-ZnO cellulose composite material on the basis of a NaOH-urea solution and application. The method comprises the step that in the NaOH-urea cellulose solution, the nano-ZnO cellulose composite material is prepared through in-situ compounding by adopting the hydrothermal method. According to the hydrothermal preparation method of the nano-ZnO cellulose composite material on the basis of the NaOH-urea solution, after cellulose is dissolved, hydroxyl groups on molecules of the cellulose are combined with zinc ions, and the defect that a zinc source does not easily penetrate into a carrier is overcome; in addition, the adopted NaOH-urea solvent is cheap and easy to obtain, and the hydrothermal synthesis temperature is obviously reduced. The nano-ZnO cellulose composite material contains 47.5% of ZnO and has the high removal efficiency on phenol in photodegradation wastewater. The ZnO cellulose composite material prepared through the method is cheap and environmentally friendly and has the very good practicability on phenolic wastewater treatment by serving as a photodegradation catalyst.

The invention discloses a preparation method of a highly stable antibacterial fruit and vegetable coating agent, and belongs to the technical field of food additive materials. According to the technical scheme, sodium-based montmorillonite is firstly used as a base carrier for synergistic bacterium resistance, meanwhile, fish enzymolysis is adopted to prepare polypeptide compound zinc chloride materials in the technical scheme, the polypeptide materials are combined through zinc, and a zinc-ion compound maintains good stability to the change of storage time; and the zinc-ion compound has certain tolerance to digestive enzymes, and the stability of zinc-ion compounding peptides is obviously higher than the stability of zinc-ion compounding glutamate. The compound coating agent prepared by the technical scheme can effectively form a coating film of a cross-linked structure through polypeptide coating, through a dense film, the effect of the environment on fruits and vegetables is well blocked, the respiration of the fruits and vegetables is effectively controlled, the film can inhibit the water loss of the fruits and vegetables during storage, the aging rate of the fruits and vegetables is slowed down, and the physical properties of the fruits and vegetables during storage are protected.

The invention relates to the field of biomedical technology, specifically, it provides a single-domain antibody specific for the zinc ion binding domain of MMP-9 protein, as well as its products and applications. The single domain antibody provided by the present invention includes complementarity determining region CDR including CDR1, CDR2 and CDR3, the amino acid sequence of CDR1 is as any one of SEQ ID NO.29-SEQ ID NO.34; the amino acid sequence of CDR2 is as SEQ ID NO. Any one of 35-SEQ ID NO.41; the amino acid sequence of CDR3 is any one of SEQ ID NO.42-SEQ ID NO.50. The single-domain antibody has obvious affinity and can replace the traditional monoclonal antibody for rapid expression to detect MMP‑9 protein or change its activity.

There is provided inter alia an aqueous liquid pharmaceutical formulation comprising (i) an insulin compound at a concentration of 500-1000 U / ml, (ii) ionic zinc, (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a logK with respect to zinc ion binding in the range 4.5-12.3 at 25° C., and (iv) a non-ionic surfactant; and wherein the formulation is substantially free of EDTA and any other zinc binding species having a logK with respect to zinc ion binding of more than 12.3 at 25° C.

The invention provides a switch-type zinc ion fluorescent probe and its preparation method and application. Sensitivity, the fluorescence of the probe is enhanced by 96 times after the probe is combined with zinc ions through the rhodamine switch ring. The preparation method of the probe is simple and suitable for scale-up synthesis and practical application. It can be used for the detection of intracellular zinc ions in the field of chemical analysis and detection, and has broad application prospects for the detection of zinc ions in other biological samples.

The invention discloses a preparation method of a strip nano-ZnO / cellulose gel material. ZnCl2 aqueous solution is used as a solvent for cellulose and a zinc source of nano-ZnO, and the cellulose fiber of a dissolved pulp is used as a cellulose raw material. Precipitation, hydrothermal synthesis and freeze-drying, a new strip-shaped nano-ZnO / cellulose gel material was prepared. The invention skillfully uses ZnCl2 aqueous solution as the solvent of cellulose and the zinc source of nanometer ZnO, therefore, no need to add other cellulose solvents, and the preparation process is clean and environment-friendly. In addition, after the cellulose is dissolved, the hydroxyl groups on its molecules combine with zinc ions to promote the formation of nano-ZnO, and the hydrothermal synthesis temperature is also significantly reduced accordingly. Therefore, the preparation process has the advantage of energy saving.

The invention discloses a zinc ion fluorescent probe as well as a preparation method and application thereof. A hemi-cyanine derivative is modified to synthesize a near-infrared fluorescent molecular probe capable of specifically reacting with zinc ions, and the fluorescent probe can generate an obvious optical effect after being combined with zinc ions, can effectively position the intra-cellular zinc ions, and can be used for detecting abnormality of intra-cellular zinc ions.

The invention provides a method for constructing a bidirectional screening system for the directional evolution of lead-binding proteins. The bidirectional screening system is formed by using the forward screening marker ampicillin resistance gene amp and the reverse screening marker fructan sucrase gene sacB. Protein PbrR and specific promoter P pbr expression regulation. According to the results of expression verification and expression optimization of the two-way screening system, the screening conditions for the binding of lead ions and lead-binding proteins are 50 μM of lead ions and 100 μg / mL of ampicillin; 10%, cells with strong binding to zinc ions inhibited growth due to sucrose sensitivity. The optimization conditions are pressure screening conditions for the directed evolution of lead-binding proteins, which are used to reduce the interference of non-target metal ions, zinc ions, and realize the specific directed evolution of lead-binding proteins; provide a new screening for the specific optimization of this type of metal regulatory proteins tool.

The present invention relates inter alia to an aqueous liquid pharmaceutical formulation comprising: (i) an insulin compound; (ii) ionic zinc; (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a log K with respect to zinc ion binding in the range 4.5-10 at 25° C.; (iv) a zinc binding species selected from species having a log K with respect to zinc ion binding of more than 12.3 at 25° C. at a concentration of less than about 0.3 mM; and (v) a non-ionic surfactant. It also provides related methods, uses and pharmaceutical compositions.

The invention belongs to the field of flame retardants, and specifically relates to a ZIFs nanosheet / chitosan / diatomite hybrid, wherein chitosan molecules are first combined with cobalt or zinc ions in ZIFs nanosheets through nitrogen elements , and then prepared by combining amino groups with hydroxyl groups on the surface of diatomite. When the hybrid is added to the polymer, chitosan can be used as an effective carbon source, and diatomaceous earth and ZIFs nanosheets have a certain physical barrier effect. At the same time, cobalt oxide or zinc oxide produced by thermal decomposition of ZIFs nanosheets and SiO 2 The acidic active sites on the surface can promote the formation of a dense carbon layer, which can play a good barrier role, thereby improving the flame retardant and smoke suppression properties of polymer materials. The invention also relates to a preparation method of the above-mentioned hybrid compound, which has low requirements for reaction, easy-to-obtain raw materials and simple process, uses water as a solvent, has no pollution to the environment, and is environmentally friendly.