31 results about "Zinc ion binding" patented technology

An article of manufacture and methods of making same. In one embodiment, the article of manufacture has a plurality of zinc oxide layers substantially in parallel, wherein each zinc oxide layer has a thickness d1, and a plurality of organic molecule layers substantially in parallel, wherein each organic molecule layer has a thickness d2 and a plurality of molecules with a functional group that is bindable to zinc ions, wherein for every pair of neighboring zinc oxide layers, one of the plurality of organic molecule layers is positioned in between the pair of neighboring zinc oxide layers to allow the functional groups of the plurality of organic molecules to bind to zinc ions in the neighboring zinc oxide layers to form a lamellar hybrid structure with a geometric periodicity d1+d2, and wherein d1 and d2 satisfy the relationship of d1≦d2≦3d1.

The invention provides a bidirectional screening system construction method for directed evolution of lead binding protein. A positive screening marker ampicillin resistance gene amp and an inverse screening marker levansucrase gene sacB are adopted to form a bidirectional screening system, and the bidirectional screening system is regulated by the lead binding protein PbrR and specific promoter Ppbr expression. Results of bidirectional screening system expression verification and expression optimization show that the screening conditions of lead ions combined with lead binding protein are 50MuM of lead ions and 100Mug / mL; the screening conditions of zinc ions and lead binding protein combined with weakened optimization pressure are 50MuM of lead ions and 10% of saccharose, and the growthof cells strongly combined with the zinc ions is inhibited due to saccharose sensitivity. The optimization condition is a pressure screening condition of directed evolution of the lead binding proteinand used for weakening the interference of non-target metal zinc ions to achieve the special directed evolution of the lead binding protein; and a new screening tool is provided for specific optimization of the metal regulator protein.

The invention relates to a histone deacetylase inhibitor and preparation and an application thereof, in particular to a novel histone deacetylase inhibitor based on a beta-elemene structure and a preparation method thereof, and further relates to an intermediate for synthesizing the histone deacetylase inhibitor and a preparation method thereof. The invention also relates to an application of the histone deacetylase inhibitor in preparation of antitumor drugs, and belongs to the technical field of chemical synthesis of drugs. The zinc ion combined compound and the salt or solvate thereof are shown as a general formula (I) or (II), wherein R, X, n and i are described in the claims and the specification.

There is provided inter alia an aqueous liquid pharmaceutical formulation comprising (i) an insulin compound, (ii) ionic zinc, (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a log K with respect to zinc ion binding in the range 4.5-12.3 at 25° C., and (iv) a non-ionic surfactant which is an alkyl glycoside; and wherein the formulation is substantially free of EDTA and any other zinc binding species having a log K with respect to zinc ion binding of more than 12.3 at 25° C.

There is provided inter aliaan aqueous liquid pharmaceutical formulationcomprising (i) an insulincompound at a concentration of 500-1000 U / ml, (ii) ionic zinc, (iii) a zinc binding species at a concentration of 1 mM or more selected from species having a logK with respect to zinc ion binding in the range 4.5-12.3 at 25 DEG C, and (iv) a non-ionic surfactant; and wherein the formulation is substantially free of EDTA and any other zinc binding species havinga logK with respect to zinc ion binding of more than 12.3at 25 DEG C.

In particular, the present invention relates to an aqueous liquid pharmaceutical formulation comprising: (i) an insulin compound; (ii) zinc ions; (iii) a concentration of 1 mM or higher selected from the group consisting of compounds having a logK for zinc ion binding at 25°C between 4.5-4.5°C. Zinc-binding substances of substances in the range of 10; (iv) zinc-binding substances selected from substances having a logK greater than 12.3 for zinc ion binding at 25° C. at a concentration of less than about 0.3 mM; and (v) non-ionic surface active agent. Related methods, uses and pharmaceutical compositions are also provided.

The invention discloses a zinc oxide antibacterial material loaded on high polymer composite bacterial cellulose, a preparation method and application thereof. The antibacterial material is added into the fermentation medium with sodium alginate or carboxymethyl cellulose as an additive, and the bacterial cellulose is combined with sodium alginate or sodium carboxymethyl cellulose during synthesis. The fermentation product has the carboxyl group in sodium alginate or sodium carboxymethyl cellulose, and the carboxyl group is used to combine with zinc ions, and the nano-zinc oxide is loaded on the polymer composite bacterial cellulose through microwave high temperature and high pressure hydrothermal reaction. The method can effectively increase the loading capacity of the nanometer zinc oxide in the bacterial cellulose, and provide stable loading. And this method is more environmentally friendly than traditional chemical grafting, and does not require the use of highly corrosive chemicals. Polymer composite bacterial cellulose-supported zinc oxide antibacterial material Bacterial cellulose with good biocompatibility has antibacterial properties and can be applied to some body surface wounds.

The invention discloses a hydrothermal preparation method of a nano-ZnO cellulose composite material on the basis of a NaOH-urea solution and application. The method comprises the step that in the NaOH-urea cellulose solution, the nano-ZnO cellulose composite material is prepared through in-situ compounding by adopting the hydrothermal method. According to the hydrothermal preparation method of the nano-ZnO cellulose composite material on the basis of the NaOH-urea solution, after cellulose is dissolved, hydroxyl groups on molecules of the cellulose are combined with zinc ions, and the defect that a zinc source does not easily penetrate into a carrier is overcome; in addition, the adopted NaOH-urea solvent is cheap and easy to obtain, and the hydrothermal synthesis temperature is obviously reduced. The nano-ZnO cellulose composite material contains 47.5% of ZnO and has the high removal efficiency on phenol in photodegradation wastewater. The ZnO cellulose composite material prepared through the method is cheap and environmentally friendly and has the very good practicability on phenolic wastewater treatment by serving as a photodegradation catalyst.

The invention relates to the field of biomedical technology, specifically, it provides a single-domain antibody specific for the zinc ion binding domain of MMP-9 protein, as well as its products and applications. The single domain antibody provided by the present invention includes complementarity determining region CDR including CDR1, CDR2 and CDR3, the amino acid sequence of CDR1 is as any one of SEQ ID NO.29-SEQ ID NO.34; the amino acid sequence of CDR2 is as SEQ ID NO. Any one of 35-SEQ ID NO.41; the amino acid sequence of CDR3 is any one of SEQ ID NO.42-SEQ ID NO.50. The single-domain antibody has obvious affinity and can replace the traditional monoclonal antibody for rapid expression to detect MMP‑9 protein or change its activity.

The invention provides a switch-type zinc ion fluorescent probe and its preparation method and application. Sensitivity, the fluorescence of the probe is enhanced by 96 times after the probe is combined with zinc ions through the rhodamine switch ring. The preparation method of the probe is simple and suitable for scale-up synthesis and practical application. It can be used for the detection of intracellular zinc ions in the field of chemical analysis and detection, and has broad application prospects for the detection of zinc ions in other biological samples.

The invention discloses a preparation method of a strip nano-ZnO / cellulose gel material. ZnCl2 aqueous solution is used as a solvent for cellulose and a zinc source of nano-ZnO, and the cellulose fiber of a dissolved pulp is used as a cellulose raw material. Precipitation, hydrothermal synthesis and freeze-drying, a new strip-shaped nano-ZnO / cellulose gel material was prepared. The invention skillfully uses ZnCl2 aqueous solution as the solvent of cellulose and the zinc source of nanometer ZnO, therefore, no need to add other cellulose solvents, and the preparation process is clean and environment-friendly. In addition, after the cellulose is dissolved, the hydroxyl groups on its molecules combine with zinc ions to promote the formation of nano-ZnO, and the hydrothermal synthesis temperature is also significantly reduced accordingly. Therefore, the preparation process has the advantage of energy saving.

The invention provides a method for constructing a bidirectional screening system for the directional evolution of lead-binding proteins. The bidirectional screening system is formed by using the forward screening marker ampicillin resistance gene amp and the reverse screening marker fructan sucrase gene sacB. Protein PbrR and specific promoter P pbr expression regulation. According to the results of expression verification and expression optimization of the two-way screening system, the screening conditions for the binding of lead ions and lead-binding proteins are 50 μM of lead ions and 100 μg / mL of ampicillin; 10%, cells with strong binding to zinc ions inhibited growth due to sucrose sensitivity. The optimization conditions are pressure screening conditions for the directed evolution of lead-binding proteins, which are used to reduce the interference of non-target metal ions, zinc ions, and realize the specific directed evolution of lead-binding proteins; provide a new screening for the specific optimization of this type of metal regulatory proteins tool.

The invention belongs to the field of flame retardants, and specifically relates to a ZIFs nanosheet / chitosan / diatomite hybrid, wherein chitosan molecules are first combined with cobalt or zinc ions in ZIFs nanosheets through nitrogen elements , and then prepared by combining amino groups with hydroxyl groups on the surface of diatomite. When the hybrid is added to the polymer, chitosan can be used as an effective carbon source, and diatomaceous earth and ZIFs nanosheets have a certain physical barrier effect. At the same time, cobalt oxide or zinc oxide produced by thermal decomposition of ZIFs nanosheets and SiO 2 The acidic active sites on the surface can promote the formation of a dense carbon layer, which can play a good barrier role, thereby improving the flame retardant and smoke suppression properties of polymer materials. The invention also relates to a preparation method of the above-mentioned hybrid compound, which has low requirements for reaction, easy-to-obtain raw materials and simple process, uses water as a solvent, has no pollution to the environment, and is environmentally friendly.