A kind of polymer composite bacterial cellulose loaded zinc oxide antibacterial material and its preparation method and application

A technology of bacterial cellulose and antibacterial materials, applied in the field of materials, can solve the problems of detachment and dissociation, bacterial cellulose without antibacterial and antibacterial, poor binding stability, etc., and achieve the effect of improving binding capacity, improving loading efficiency, and simple operation

Active Publication Date: 2021-08-31
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the clinical process, body surface wounds are often accompanied by infection, and bacterial cellulose itself has no antibacterial and antibacterial ability, so it is difficult to be used in body surface wounds. Therefore, the needs of clinical practice put forward new requirements for bacterial cellulose wound dressings , there is an urgent need for a bacterial cellulose material with good antibacterial properties
[0003] In the prior art, the antibacterial and antibacterial function of bacterial cellulose is generally realized by loading nano silver by soaking method. Although the antibacterial properties of nano silver have been widely recognized, the bioaccumulation and toxic side effects caused by its long-term and large-scale use can no longer be ignored. ; and the method of soaking is adopted to realize the loading of nanomaterials through osmosis. On the one hand, the loading capacity of nanomaterials is very limited, and on the other hand, the combination stability of nanomaterials in bacterial cellulose is poor, and it is easy to fall off and free

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Seed solution preparation: get two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1 and insert them into a 300mL triangular flask equipped with 50mL of liquid activation medium (liquid activation medium per liter of formula group The ingredients are as follows: glucose 20g, peptone 5g, yeast extract 5g, disodium hydrogen phosphate 2.7g, citric acid 1.15g, solvent is water, pH value is 6.0; preparation: mix and dissolve all ingredients in water, sterilize and cool to get ), 28°C, 100rpm, stroke 65mm, shaking culture for 36h;

[0027] (2) Fermentation of bacterial cellulose: Take 1mL seed liquid and add 100mL Erlenmeyer flask with 20mL fermentation medium (the fermentation medium contains 20g of glucose per liter, 0.8g of yeast extract, 6g of peptone, 6g of disodium hydrogen phosphate, biochemical grade 4g of corn steep liquor, 0.8g of acetic acid, 8g of absolute ethanol, 1g of sodium alginate with a viscosity of 500mPa·s, the balance is water, and th...

Embodiment 2

[0034] (1) Seed solution preparation: get two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1 and insert them into a 300mL triangular flask equipped with 50mL of liquid activation medium (liquid activation medium per liter of formula group The ingredients are as follows: glucose 20g, peptone 5g, yeast extract 5g, disodium hydrogen phosphate 2.7g, citric acid 1.15g, solvent is water, pH value is 6.0; preparation: mix and dissolve all ingredients in water, sterilize and cool to get ), 28°C, 100rpm, stroke 65mm, shaking culture for 36h;

[0035] (2) Fermentation of bacterial cellulose: Take 2mL seed liquid and add 100mL Erlenmeyer flask with 20mL fermentation medium (fermentation medium contains 60g of glucose per liter, 1.2g of yeast extract, 8g of peptone, 10g of disodium hydrogen phosphate, biochemical grade 8g of corn steep liquor, 1.2g of acetic acid, 12g of absolute ethanol, 20g of sodium carboxymethyl cellulose with a viscosity of 50mPa·s, the balance is ...

Embodiment 3

[0042] (1) Seed solution preparation: get two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1 and insert them into a 300mL triangular flask equipped with 50mL of liquid activation medium (liquid activation medium per liter of formula group The ingredients are as follows: glucose 20g, peptone 5g, yeast extract 5g, disodium hydrogen phosphate 2.7g, citric acid 1.15g, solvent is water, pH value is 6.0; preparation: mix and dissolve all ingredients in water, sterilize and cool to get ), 28°C, 100rpm, stroke 65mm, shaking culture for 36h;

[0043] (2) Fermentation of bacterial cellulose: Take 1mL seed liquid and add 100mL Erlenmeyer flask with 20mL fermentation medium (fermentation medium contains 40g of glucose per liter, 1g of yeast extract, 7g of peptone, 8g of disodium hydrogen phosphate, biochemical grade corn 5g of slurry, 1g of acetic acid, 10g of absolute ethanol, 2g of sodium carboxymethylcellulose with a viscosity of 4500mPa·s, the balance is water, and ...

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Abstract

The invention discloses a zinc oxide antibacterial material loaded on high polymer composite bacterial cellulose, a preparation method and application thereof. The antibacterial material is added into the fermentation medium with sodium alginate or carboxymethyl cellulose as an additive, and the bacterial cellulose is combined with sodium alginate or sodium carboxymethyl cellulose during synthesis. The fermentation product has the carboxyl group in sodium alginate or sodium carboxymethyl cellulose, and the carboxyl group is used to combine with zinc ions, and the nano-zinc oxide is loaded on the polymer composite bacterial cellulose through microwave high temperature and high pressure hydrothermal reaction. The method can effectively increase the loading capacity of the nanometer zinc oxide in the bacterial cellulose, and provide stable loading. And this method is more environmentally friendly than traditional chemical grafting, and does not require the use of highly corrosive chemicals. Polymer composite bacterial cellulose-supported zinc oxide antibacterial material Bacterial cellulose with good biocompatibility has antibacterial properties and can be applied to some body surface wounds.

Description

technical field [0001] The invention belongs to the field of materials, and in particular relates to a high-polymer composite bacterial cellulose-supported zinc oxide antibacterial material and a preparation method and application thereof. Background technique [0002] Bacterial cellulose (BC) is formed by the growth and assembly of bacteria itself, and has a natural nanometer three-dimensional network structure. Bacterial cellulose has a high ability to bind water, has a larger surface area than plant fibers, and has a higher chemical adsorption capacity. Bacterial cellulose has high biocompatibility, adaptability and good biodegradability, and is widely used in various fields of tissue engineering. Due to the good mechanical properties and excellent water retention capacity of bacterial cellulose materials, their application in wound dressings has attracted great attention. In the clinical process, body surface wounds are often accompanied by infection, and bacterial cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/16A01N25/10A01P3/00A01P1/00A61L15/18A61L15/28A61L15/46
CPCA01N25/10A01N59/16A61L15/18A61L15/28A61L15/46A61L2300/102A61L2300/404A61L2400/12C08L1/02C08L5/04C08L1/286
Inventor 冯劲施庆珊张丹丹王倩彭如群周刚谢小保
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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