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Preparation method and application of high-water solubility oyster zinc chelate peptide

A chelating peptide, water-soluble technology, applied in the field of preparation of oyster zinc chelating peptide, can solve the problems of biological effects accompanied by side effects, etc., and achieve the effect of improving zinc ion binding ability, small molecular weight, and good solubility

Pending Publication Date: 2019-11-12
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The earliest zinc supplements are inorganic supplements such as zinc sulfate, which are simple in structure and low in price. Although taking an appropriate amount can eliminate the symptoms caused by zinc deficiency, they are often accompanied by side effects and have low biological effects after taking them.

Method used

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  • Preparation method and application of high-water solubility oyster zinc chelate peptide
  • Preparation method and application of high-water solubility oyster zinc chelate peptide
  • Preparation method and application of high-water solubility oyster zinc chelate peptide

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specific Embodiment approach 1

[0046] Embodiment one: the preparation method of the oyster zinc chelating peptide with high water solubility in this embodiment is realized in the following steps:

[0047] Fresh oyster meat is cleaned, and dried by vacuum freeze drying to obtain oyster freeze-dried powder;

[0048] Mix the oyster freeze-dried powder obtained in step 1 with deionized water at a ratio of 1:3 to 1:5, use an alkaline regulator to adjust the pH of the system to 12 to 13, stir for 2h to 3h, and set the temperature at 1000rpm to 12000rpm Centrifuge for 15min to 20min, and take the supernatant. Use an acidic regulator to adjust the pH of the system to 4.8-5.1, let it stand for 1h-2h, centrifuge at 10000rpm-12000rpm for 15min-20min to collect the precipitate, and then use vacuum freeze-drying to obtain oyster protein powder;

[0049] Calculated according to the protein content of 80%, use the oyster protein powder obtained in step 2 to prepare an oyster protein solution with a protein concentration ...

specific Embodiment approach 2

[0054] Embodiment 2: This embodiment differs from Embodiment 1 in that step 2 mixes oyster freeze-dried powder and deionized water in a ratio of 1:3. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0055] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that step two is centrifuged at 10,000 rpm for 15 minutes to get the supernatant. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a preparation method and application of oyster zinc chelate peptide with high water solubility. Oyster meat is used as raw material. The method comprises the following steps of: preparing oyster freeze-dried powder, extracting oyster protein, carrying out alkaline protease enzymolysis, carrying out 3KDa ultrafiltration centrifugation, carrying out SPE column purification bytaking C18 as a filler, and carrying out vacuum freeze drying to obtain the high-water solubility oyster zinc chelate peptide. The preparation method is simple and safe. The prepared high-water solubility oyster zinc chelate peptide is a natural edible component, has higher zinc ion binding capacity and high water solubility, has good solubility, is easy to absorb, can be used as a zinc supplement, and has an application prospect of practical development.

Description

technical field [0001] The invention relates to a preparation method of a bioactive peptide, in particular to a preparation method and application of an oyster zinc chelating peptide with high water solubility. Background technique [0002] Oysters, commonly known as oysters or sea oysters, are one of the largest seafood resources in my country. They contain a high level of protein, as well as various vitamins and trace elements necessary for the human body. Under the best enzymatic hydrolysis conditions, the crude protein content of the oyster protein isolate is as high as 81.7%, the total sugar content is 2.0%, the ash content is 4.2%, and the essential amino acids in the protein isolate account for 42.44% of the total amino acids. Because of its smaller molecular weight, oyster polypeptides are easier for the human body to digest and absorb than macromolecular proteins. Many studies have shown that oyster polypeptide has various functional activities such as anti-oxidati...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16C07K1/14A23L33/18A23L33/165
CPCC12P21/06C07K1/36C07K1/34C07K1/16C07K1/14A23L33/18A23L33/165
Inventor 杜明王子烨王震宇陈慧许喆吴迪叶灏铎李国栋
Owner DALIAN POLYTECHNIC UNIVERSITY
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