Porcine contagious pleuropneumonia actinobacillus serum 1-type zinc ion binding protein A deletion strain, construction method therefor and applications thereof

Abstract

The invention relates to a porcine contagious pleuropneumonia actinobacillus serum 1-type zinc ion binding protein A (znuA) deletion strain, a construction method therefor and applications thereof. The microbial colllection number is CCTCC No: M2012476. The classification and nomenclature is inobacillus pleuropneumoniae APP-1( delta znuA). The collection time is November 23th, 2012. The collection place is China Center for Type Culture Collection. The collection address is Wuhan University, Wuhan, China. The znuA gene deletion mutantion strain is compared to the parent strain to study biological characteristics and pathogenic immunogenicity. The discoveries show that the pathogenicity of the gene deletion strain is obviously weaker than that of the parent strain APP. The gene deletion strain can be used for developing gene deletion inactivated vaccines of APP, and provides effective technologies and methods for preventing and treating porcine contagious pleuropneumonia safely and effectively.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a deletion strain of Actinobacillus pleuropneumoniae serum type 1 zinc ion binding protein A (znuA) and its construction method and application. Background technique [0002] Porcine contagious pleuropneumonia (PCP) is a highly contagious respiratory disease caused by Actinobacillus pleuropneumoniae (APP). The disease mainly causes hemorrhagic and necrotizing pneumonia accompanied by pleurisy in pigs, and most of them are the most acute or acute course and quickly die. The disease can occur in pigs of any age and is prevalent all over the world. Once an outbreak occurs, the economic loss will be huge. The current domestic control of porcine pleuropneumonia mainly relies on inactivated vaccine immunization and antibiotic treatment. Although inactivated vaccines can effectively control the infection of homologous strains, due to the large number of serotypes and complex a...

AI Technical Summary

Problems solved by technology

Although inactivated vaccines can effectively control the infection of homologous strains, due to the large number of serotypes and complex antigenic components, the control effect on heterologous strains is not satisfactory

The abuse of antibiotics has led to the emergence of a large number of drug-resistant strains in my country, increasing the risk of multi-drug resistance, making disease prevention and treatment more difficult, and easily leading to excessive drug residues in pork products and causing public health problems

Images