148 results about "Trichoderma virens" patented technology

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a microorganism bacterium agent for straw and excrement mixed composting. The microorganism bacterium agent is composed of the following strains: (2-6)*10<10>cfu of hair mould per gram, (2-6)*10<10>cfu of trichoderma virens per gram, (2-6)*10<10>cfu of geotrichum candidum per gram, (2-8)*10<9>cfu of saccharomyces cerevisiae per gram, (3-5)*10<11>cfu of bacillus thermophilus per gram, (2-6)*10<11>cfu of lactobacillus thermophilus per gram, (2-8)*10<10>cfu of white rot fungi per gram, (2-8)*10<10>cfu of monilinia fructicola per gram, (3-7)*10<8>cfu of pseudomonas putida per gram, (2-6)*10<11>cfu of nitrosococcus per gram, (1-4)*10<6>cfu of green algae per gram and (2-5)*10<7>cfu of blue-green algae per gram. The microorganism bacterium agent can promote a straw and excrement mixed aerobatic composting progress, shorten a composting period, improve a composting rotten degree, accelerate biodegradation of organic matters, alleviate ozone generation and diffusion during composting and reduce loss of nutrient substances such as nitrogen, can be applied to composting of different straw and excrement raw materials for composting enterprises and is used for producing bio-organic fertilizers.

The invention discloses a crop straw decomposing inoculant and application in straw bioreactor and water-logged compost. The inoculant is compounded of 0.5-2 parts of phanerochaete chrysosporium, 0.5-2 parts of trichoderma aureoviride, 0.5-2 parts of bacillus subtilis and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The straw decomposing inoculant is applied to a tomato stalk bioreactor planting technology, and the average ground temperature is 18.3 DEG C and is increased by 2.2 DEG C as compared with that of contrast without use of the inoculant; the average greenhouse temperature is 14.3 DEG C and is increased by 2.0 DEG C as compared with the average greenhouse temperature of the contrast; the average CO2 concentration is 956ppm and is 2.17 times higher than that of the contrast; the harvest time is 15 days earlier than the harvest time of the contrast; the acre yield is 5881kg and is increased by 22.7 percent as compared with the acre yield of the contrast; the occurrence rate of plant diseases and insect pests is lower as compared with that of the contrast. The inoculant is applied to wheat straw retting and decomposing, is high in decomposition speed, high in compost flexibility, the straws are basically decomposed, and the fermentation time is shorter than that of an inoculant purchased in the market.

The invention relates to the field of algae inhibitors, the invention specifically relates to a sesquiterpene alcohol compound derived from algal epiphytic fungus, a preparation method thereof and anapplication of the sesquiterpene alcohol compound in the aspect of inhibiting microalgae. The specific structural formula is shown in the formula (I). The preparation method comprises the following steps: inoculating a fungal culture medium with Trichoderma virens Y13-3 for fermentation culture, and subjecting a fermentation product to separating and purifying, and the product is namely a sesquiterpene alcohol compound as shown in the formula (I). The median inhibition concentration of the sesquiterpene alcohol compound to the microalga can be up to 1.2 microgram / ml through the microalga activity inhibition experiment.

The invention discloses a straw organic fertilizer and a preparation method thereof. The preparation method is to use a microbial inoculum used for degrading straw to ferment straw so as to obtain thefertilizer; the active ingredients of the microbial inoculum for degrading straw comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratioof Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The fertilizer used for degrading straw can be used as a base fertilizer and can obviously improve the quality of vegetables.

The invention relates to a trichoderma atroviride B-8-1-34 strain capable of highly yielding cellulose and application of the trichoderma atroviride B-8-1-34 strain in the preparation of the cellulose, belonging to the technical field of microorganisms. The trichoderma atroviride B-8-1-34 strain disclosed by the invention has been preserved in China General Microbiological Culture Collection Center on Aug.10, 2012, with the preservation number of CGMCC No.6390. The trichoderma atroviride B-8-1-34 disclosed by the invention can be used for producing the cellulose by making use of lignocellulose-containing industrial and agricultural wastes in combination of low-cost raw materials such as bran, maize slurry and soybean cake powder through liquid or solid fermentation, has the advantages of short fermentation period, high activity of produced cellulose, reasonable components and high lignocellulose hydrolysis, and provides a novel strain resource to solve the problems on high production cost, low saccharifying efficiency and the like caused by low enzyme activity and unreasonable enzyme components in the utilization of cellulose resources.

The invention relates to application of a biocontrol fungus Trichoderma virens SS161 and metabolites thereof, belonging to the technical field of microorganisms. The collection number of the biocontrol fungus Trichoderma virens SS161 provided by the invention is CGMCC No.7224. The strain is separated from a rotten rice straw in a rice seedling breeding disc, and shows high antagonistic action on multiple pathogenetic fungi. A fermentation product of the strain has growth promoting action on rice and contains antibacterial substances. The separation of the strain provides an excellent original strain for the research and development of microorganism-derived agricultural chemicals.

The invention provides a pig manure and medicine slag fermentation organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizerpreparation. The organic fertilizer is prepared from the following raw materials in parts by weight through fermentation: 50 to 70 parts of pig manure, 25 to 40 parts of thoroughly decomposed anemarrhena pill medicine slag, 10 to 30 parts of corn stalk subjected to blasting pretreatment, 10 to 20 parts of oyster shell powder, 5 to 8 parts of potassium fulvic acid, 3 to 5 parts of molasses, 1.5 to3.5 parts of EM germ compost fermenting agents and 0.02 to 0.07 part of cellulase. The enzyme activity of the cellulase is one hundred thousand to two hundred thousand U / g, wherein the thoroughly decomposed anemarrhena pill medicine slag is obtained by fermenting and thoroughly decomposing medicine slag through trichoderma virens F7 strains; the preservation code of the trichoderma virens F7 strains is CGMCC 3.17613. The organic fertilizer is prepared from the raw materials through twice fermentation. The organic fertilizer has rich nutrition; the nutrition proportioning is reasonable; the organic fertilizer can be applied to crop planting.

The invention provides crop growth promoting antagonistic trichoderma, in particular to Trichoderma atroviride H18-1-1 and TF28 with preservation numbers of CGMCC No.9774 and 9775 and aims to solve the problem that using agricultural chemicals for preventing and treating agricultural diseases is prone to causing environment pollution and agricultural chemical residues, jeopardizing an agricultural ecological system, killing beneficial microorganisms in the environment, improving drug resistance of phytopathogen and finally causing decline of prevention and treatment effects and even failure in prevention and treatment. The trichoderma not only can be applied to agricultural biological prevention and treatment but also can promote growth of crops such as wheat and prevent and treat diseases caused by rhizoctonia cerealis, fusarium culmorum, fusarium proliferatum and / or fusarium oxysporum and the like.

The invention belongs to the technical field of the agricultural microbiology and discloses a complex bacterial preparation and application thereof. The complex bacterial preparation is prepared by mixing the following components: 1 to 3 parts of fermentation liquor of Escherichia.coli, 2 to 3 parts of fermentation liquor of Trichoderma virens, 1 to 4 parts of fermentation liquor of Pseudomonas fluorescens and 4 to 6 parts of fermentation liquor of Bacillus subtilis. The complex bacterial preparation provided by the invention not only can improve the methane yield of straws and cow dung and improve the methane generating speed, but also can further reduce content of solid waste by 30 percent. The method has the advantages of quick response, low cost, simple using method, easy popularization and the like and can be popularized and used.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, can survive for a long term in a plurality of improved soil to play a role, is good in adaptation of improving soil / land and especially can be used for effectively improving a plurality of soil, such as secondary salinization land, desertification soil.

The invention relates to the technical field of crop fertilizers, and particularly relates to a special stubble-resistant biological bacterial fertilizer for radix salviae miltiorrhizae. The special stubble-resistant biological bacterial fertilizer comprises the following components in percentage by weight: 8%-12% of microbial agents, 40%-50% of fresh pepper seeds, 40%-50% of organic substances and 2%-5% of trace elements. According to the special stubble-resistant biological bacterial fertilizer, the problems of serious blight, root rot and root-knot nematode disease, which are caused due to years of continuous cropping, of the radix salviae miltiorrhizae are well solved, so that the output and quality of the radix salviae miltiorrhizae are increased; the double roles of sterilization and protection are played by adopting a trichoderma aureoviride W2256 strain, so that the soil is improved, and the environment is protected; the addition of a pure natural organic fertilizer is favorable to the growth of trichoderma, and the pure natural organic fertilizer and the trichoderma complement each other; a raw material is a pure biological raw material without pollution on the environment and crops, so that the content of heavy metals contained in the soil can be effectively reduced, and the content of organic matters contained in the soil can be increased; in addition, the reasonable formula, equivalent nutrition and better using effect are achieved, the utilization ratio of the fertilizer can be obviously enhanced, and the output and quality of the radix salviae miltiorrhizae can be increased.

The invention discloses a preparation technology of fly maggot chitin and chitosan and an application of fly maggot chitin to inducing a trichoderma aureoviride T2 bacterial strain to generate chitinase, belonging to the field of biological technology. The preparation technology of fly maggot chitin has the main characteristics that the traditional hydrochloric acid calcium removal technology is changed into an EDTA (Ethylene Diamine Tetraacetic Acid) calcium removal technology, the environmental pollution caused by waste acid discharge can be reduced, the damage of concentrated acid on chitin molecular chains can be avoided, and the chitin product with the high quality is obtained; and according to the preparation technology of fly maggot chitin, the traditional technology of 4-hour high-temperature concentrated alkaline refluxing is changed into a sodium hydroxide-amyl alcohol reaction system, microwave heating treatment is carried out twice, the utilization rate of alkali liquid is increased, the reaction time is greatly shortened, the energy consumption is saved, and a white powdery chitosan product with the lower moisture content and the higher degree of deacetylation is obtained. The fly maggot chitin disclosed by the invention can be used as a carbon source for inducing the trichoderma aureoviride T2 bacterial strain to generate the chitinase and has obvious induction effect.

The invention discloses an organic special fertilizer capable of enhancing sweetness of citrus fruits and enabling citruses to have high yield, for planting citruses. The organic special fertilizer is prepared from the following raw materials in parts by weight: 30-50 parts of vinegar dregs, 30-50 parts of Maya blue powder, 65-105 parts of livestock manure, 20-40 parts of nostoc sphaeroides yeast powder, 15-20 parts of bamboo pulp dregs, 5-10 parts of EDTA ferric sodium salt, 10-20 parts of sugar alcohol magnesium, 10-15 parts of crop straw powder, 6-10 parts of dried stevia rebaudiana leaves, 3-6 parts of abandoned lime peel, 3-6 parts of durian shells, 10-20 parts of extracted leaf residues of stevia rebaudiana, 10-30 parts of red jujube leaves, 0.1-0.5 part of nanometer activated carbon, 0.4-0.5 part of photosynthetic induction factors, 2-6 parts of bacillus subtilis microbial inoculum, 1-5 parts of corynebacterium glutamicum, 3-5 parts of trichoderma virens, 3-5 parts of phosphate solubilizing bacteria, 1-3 parts of potassium-releasing bacteria and the like. The organic special fertilizer disclosed by the invention has various functions of increasing yield, enhancing sweetness, improving soil, restraining soil-borne diseases and the like.

The invention relates to the technical field of biopesticides, and particularly relates to application of trichoderma atroviride HB20111 to prevention and treatment of wheat stem rot and banded sclerotial blight. The trichoderma atroviride HB20111 is preserved in China General Microbiological Culture Collection Center on December 5, 2018, and the preservation number of the trichoderma atroviride HB20111 is CGMCC No. 16963. The invention belongs to the field of green biopesticides, and the use of the trichoderma atroviride HB20111 can correspondingly reduce the use of chemical pesticides and pesticide residues in agricultural products and has important significance for improving the quality of the agricultural products and is suitable for production and application of pollution-free green agricultural products.

A seed treated with a fungal / bacterial antagonist combination and a seed assembly comprising a seed and a fungal / bacterial antagonist combination. The fungal / bacterial antagonist combination comprises a Trichoderma virens fungal antagonist and a Bacillus amyloliquefaciens bacterial antagonist for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. In preferred embodiments, the invention produces an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention belongs to the field of microorganisms and microorganism application, and in particular relates to a trichoderma aureoviride strain TW351 (Trichodermaaureoviridesp.) and application of the trichoderma aureoviride strain in inhibition against plant diseases. The strain is stored in China Center for Type Culture Collection in Oct., 17th in 2012, and the preservation number is CCTCC NO:M2012407; the strain can quickly grow on a personal digital assistant (PDA) solid culture medium under the temperature of 28 DEG C; after 48 hours, the radius of a bacterial colony is 3.4 cm; after 72 hours, the radius of the bacterial colony is 5.4 cm; the form of the strain under an optical microscope is that formed mycelium is white or colorless and is in a short fur type; after being matured, the bacterial colony is light yellow green. When ginger seeds are planted, the seed gingers are soaked in a trichoderma preparation according to the proportion of the preparation to water of 1:100; and therefore, the ginger damping off disease prevention rate is up to 82 percent. When the damping off diseases are prevented, effects for accelerating germination of the ginger seeds and hestening the rooting of the ginger seeds can be achieved.

The combination of a Trichoderma virens species and a rhizosphere competent Trichoderma harzianum species is used to control plant diseases and enhance plant growth.

The invention discloses a compound which is derived from trichoderma virens and has nematicidal activity as well as a preparation method and an application thereof. The compound viridiol has a structure as shown in a formula (I). The compound disclosed by the invention has strong killing effects on various nematodes such as soybean cyst nematode, pine wood nematode, meloidogyne javanica and meloidogyne incognita, can be applied to making preparations for preventing and controlling nematodes, and has the advantages of high efficiency, low toxicity, broad spectrum, good industrial prospect and the like.

The invention discloses a microorganism-based traditional Chinese medicine wastewater treatment agent, which is composed of A component and B component, and the A component is prepared from the following raw materials in parts by weight: Trichoderma viridans powder 0.3-0.5 part, Rhodopseudomonas marsh 0.2-0.3 parts of bacteria powder, 0.05-0.2 parts of Enterobacter YC105C bacteria powder, 0.05-0.3 parts of Thiobacillus bacteria powder, 5-10 parts of Maya blue powder, 1-6 parts of activated carbon fiber, 3-7 parts of furfural activated carbon, sharp Titanium-type sulfur-lanthanum co-doped nano-titanium dioxide 1-3 parts, Bacillus amyloliquefaciens 0.04-0.1 parts, sulfonic acid bacteria 0.05-0.1 parts, sodium alginate solution 10-20 parts, calcium chloride solution 10-20 parts; Component B includes cellulase, protease, xylanase, laccase and pectinase. The invention can degrade high-molecular organic matter and convert all of them into CO 2 , water and other small molecular substances, strong degradation ability, no secondary pollution.

The invention provides a straw returning cellulose-decomposing biological control fungus, a microbe and application of the microbe, wherein the strain F7JX993849 is classified and named as trichoderma virens which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 3.17613. The trichoderma virens F7JX993849 is relatively strong in decomposing capacity simultaneously on cellulose, hemicellulose and pectin components in crop straw. In addition, the trichoderma virens F7JX993849 is capable of antagonizing the invasion of phytopathogenic fungi, and has a biological control function. Furthermore, the microbe prepared from the straw returning cellulose-decomposing biological control fungus disclosed by the invention can be used for solving the problems of a general straw decomposition agent which is un-ideal in effect and is instable in the process of application; and the microbe has a good application value.

A seed treated with a fungal / bacterial antagonist combination and a seed assembly comprising a seed and a fungal / bacterial antagonist combination. The fungal / bacterial antagonist combination comprises a Trichoderma virens fungal antagonist and a Bacillus amyloliquefaciens bacterial antagonist for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. In preferred embodiments, the invention produces an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

A composition of matter comprising: a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi that is placed in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root; and a method for increasing plant yield comprising: placing a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root.

The invention discloses a co-culture method of trichoderma atroviride and bacillus subtilis and an application of a co-metabolite obtained by the method. According to the method, the trichoderma atroviride SG3403 and bacillus subtilis 22 strains are used as co-culture objects, the inhibition rate of a co-culture fermentation liquid against fusarium graminearum is used as a response value, and thefermentation conditions such as culture temperature, a rotation speed, a pH, a corn flour content, a molasses content, a yeast powder content and a KCl content are optimized by a response surface method to obtain the following best fermentation conditions: the culture temperature is 28 DEG C, the rotation speed is 214 rpm, the pH value is 6.8, the yeast powder content is 10 g / L, and the molasses content is 27.6 g / L. The co-culture fermentation liquid cultivated by the formula provided by the invention improves the resistance to the fusarium graminearum, a substance not found in a single culture is found in the co-culture fermentation liquid, a seed coating agent prepared from the substance promotes the growth of wheat, and a novel option is provided for the development of biological pesticides and biological seed coating agents.

The invention relates to the technical field of microbial medicines, in particular to a cadinane type sesquiterpenoid compound obtained from a fermentation product of Trichoderma virens, and a separation and purification method and application thereof. The molecular formulas of the sesquiterpene compound are C15H16O3 (compound 1) and C15H22O2 (compound 2), respectively, as shown in formulas 1 and2. The compounds 1 and 2 obtained by the invention have relatively good and broad-spectrum antibacterial activity and are expected to be further developed into a suppressing drug for treating infections caused by various bacteria or fungi. The formula is shown in the description.

The invention relates to a remediation method for copper and cadmium co-contaminated soil. The method specifically comprises the steps that eleusine indica is planted in the contaminated soil, and Trichoderma virens F7 or inactivation Trichoderma virens F7 are applied in a combined manner; and the classification name of the Trichoderma virens F7 is Trichoderma virens, and is stored in the ordinary microorganism center of the Chinese microorganism culture preservation administration committee with the storage number of CGMCC No.3.17613. The contaminated soil is treated with the Trichoderma virens F7 combined with the eleusine indica; remediation efficiency for the copper and cadmium co-contaminated soil is improved; and a cooperative remediation effect improvement phenomenon exists between microorganisms and the plants; a good ecological effect is provided, and the remediation method is applicable to remediation of the copper and cadmium co-contaminated soil; the application method for Trichoderma virens F7 fermentation liquor and solid preparations is easy; the biomass of the eleusine indica is obviously increased; meanwhile, the microbial biomass of the soil can be increased; and the rhizosphere micro-ecological environment of the plants is improved.