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Control of plant diseases and enhancing plant growth using a combination of a trichoderma virens species and a rhizosphere competent trichoderma harzianum species

a technology of trichoderma virens and rhizosphere, which is applied in the field of enhancing plant growth and controlling plant diseases, can solve the problems of increasing the productivity of targeted plants and lowering the buying cost of having to use multiple biocontrol agents, so as to improve the productivity of targeted plants and enhance the effect of disease protection

Inactive Publication Date: 2010-02-04
BIOWORKS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0022]Because of multiple shortcomings observed with individual or specific biocontrol agents, there is much need for a diverse agent capable of treating various Phytophthora spp. This will not only increase productivity of the targeted plants, but will also potentially lower the buying cost of having to use multiple biocontrol agents.
[0023]The combination of rhizosphere competent T. harzianum and T. virens prevents plants from becoming diseased and performs better than either organism alone; there is an enhanced effect on disease protection when the two are combined. Further, the combination performed as well as chemical fungicides. In particular, plants treated with this combination showed no symptoms or less severe symptoms of pathogen infection and had greater root mass, grew taller, and were more marketable than plants treated with either biological agent alone. Diseased plants typically exhibit multiple symptoms, generally associated with lack of root growth, and eventually, root death.
[0024]Growers and consumers are interested in reducing the use of chemical pesticides in agricultural crops and seek alternative materials for pest management. Effective biological control materials that have a low impact on the environment and non-target organisms can be used as alternatives to, or in a program along with, traditional chemical pesticides. The present invention provides a broad-spectrum control of multiple diseases in agronomic crops and a safe alternative to chemical pesticides.

Problems solved by technology

This will not only increase productivity of the targeted plants, but will also potentially lower the buying cost of having to use multiple biocontrol agents.

Method used

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  • Control of plant diseases and enhancing plant growth using a combination of a trichoderma virens species and a rhizosphere competent trichoderma harzianum species

Examples

Experimental program
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Effect test

example 1

Treatment of Boxwoods Infected with P. cinnamomi

[0051]This example gives an illustration of the efficacy and synergy of a composition according to the present invention for controlling Phytophthora cinnamomi on boxwood plants.

[0052]Boxwoods, Buxus microphylla asiaticum, var. ‘Winter Gem’ were subjected to the soil drench treatments set forth in Table 1. Except for the non-inoculated controls, each plant was treated with P. cinnamomi inoculum to induce disease. Boxwood plants were obtained from a local nursery and placed into a 6 inch diameter pot containing pine bark: peat (3:1 volume:volume) potting media. The medium was amended with 14 lb of 17-7-12 Osmocote fertilizer per cubic yard of mix.

[0053]Inoculum of P. cinnamomi was grown on sterilized rice grains for 14 days at 24-26° C. prior to inoculation. To treat the plants with the pathogen inoculum, four holes were punched equidistant around the root ball, and four colonized rice grains were inserted in each hole. Each of the tes...

example 2

Treatment of Boxwoods Challenged with Phytophthora cinnamomi

[0065]This example gives an illustration of the efficacy and synergy of a composition according to the present invention for controlling Phytophthora cinnamomi on boxwood plants.

[0066]Boxwoods were subjected to the spray treatments set forth in Table 5. Except for the non-inoculated controls, each plant was treated with P. cinnamomi inoculum to induce disease. The methodology for this test was identical to that described in Example 1.

TABLE 5TreatmentRate1. Non-Inoculated Control—2. Inoculated Control—3. G41 (Trichoderma virens)4 oz / 100 gal4. G41 + T224 oz + 4 oz / 100 gal5. Mefenoxam1 oz / 100 gal

[0067]Marketability results are set forth in Table 6. The combined treatment of T22 and G41 prevented the plants from developing disease symptoms and marketability remained high over time, particularly when compared to these treatments administered separately.

TABLE 6Marketability: 1(dead)-5(excellent) ScaleNov. 21,Dec. 12,Jan. 13,Trea...

example 3

Treatment of Azaleas Challenged with Phytophthora nicotianae

[0070]This example gives an illustration of the efficacy and synergy of a composition according to the present invention for controlling Phytophthora nicotianae on azalea plants.

[0071]Azalea, Rhododendron obtusum, var. ‘Pink Happy Days’ plants were subjected to the spray treatments set forth in Table 9. Except for the non-inoculated controls, each plant was treated with P. nicotianae inoculum to induce disease. The methodology for this test was identical to that described in Example 1.

TABLE 9TreatmentRate1. Non-Inoculated Control—2. Inoculated Control—3. G41 (Trichoderma virens)4 oz / 100 gal4. G41 + T224 oz + 4 oz / 100 gal5. Mefenoxam1 oz / 100 gal

[0072]Marketability results are set forth in Table 10. Marketability was maintained over the course of the experiment in plants treated with the combination of T22 and G41 biocontrol agents; protection from disease symptoms was equal to that of the chemical standard.

TABLE 10Marketabi...

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Abstract

The combination of a Trichoderma virens species and a rhizosphere competent Trichoderma harzianum species is used to control plant diseases and enhance plant growth.

Description

[0001]This application claims benefit of U.S. Provisional Patent Application Ser. No. 61 / 081,497, filed Jul. 17, 2008, which is hereby incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to the control of plant diseases and enhancing plant growth using a combination of a rhizosphere competent Trichoderma harzianum species and a Trichoderma virens species.BACKGROUND OF THE INVENTION[0003]Phytophthora spp are pathogenic agents from the family of Pythiaceae known to be involved in various plant diseases. Sudden oak death, soybean root rot, apple crown and collar rot, root rot, affecting American chestnuts, rhododendron, African violet, or strawberries, are just examples of diseases caused by this group of pathogens. Typically, plant diseases caused by Phytophthora are considerably difficult to control and often lead to the death of the plant. This pathogen is a widespread and an economic problem to growers around the world. For example, P...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/38
CPCA01N63/04C12N1/14A01N63/38A01N65/00
Inventor MARTIN, JR., WILLIAM RANDOLPHHAYES, CHRISTOPHER KENT
Owner BIOWORKS
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