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Trichoderma aureoviride strain and application thereof

A technology of Trichoderma yellow green strains, applied in the application, fungicides, fungi and other directions, can solve the problems of seed rot, dwarf, germination rate decline and so on

Active Publication Date: 2013-09-25
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when Fusarium equisetum infects the rhizome of seedlings, it can also cause seed rot or seedling damping; after Fusarium moniliforme infection, the germination rate of seeds decreases by about 40%, or abnormal seedlings are formed, such as cotyledons and seed coats Covered by hyphae, the seed coat cannot fall off, and the cotyledons cannot be stretched; after the Fusarium oxysporum infects the seeds, the germination rate decreases by 62%, and the seedlings are dwarfed, the stems are constricted, bent, or the cotyledons are drooping and other deformities When the seedlings emerge, the main root turns yellowish brown, and the secondary root is necrotic and falls off; after the Fusarium solani fungus infects the seeds, the germination rate decreases by 52%. or dwarf

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Trichoderma chrysogenum fungus strain TW351 ( Trichoderma aureoviride sp. ), the strain was preserved in the China Center for Type Culture Collection on October 17, 2012, and its preservation number is CCTCC NO: M2012407. The strain grows on PDA solid medium, grows fast at 28°C, 48 hours, and the colony radius up to 3.4 cm, and up to 5.4 cm in 72 hours; the morphology under the optical microscope is: the formed hyphae are white or colorless, short fluffy, and the colony becomes light yellow-green after maturing; the growth of the colony and the production of conidia are almost simultaneous , sporulation forms a large amount of spore powder covering the colony, the spores are green, the main branch of the conidiophores is slender, and the secondary branches form a group of 2-3; the phialides are long and thin, and the base does not become thin, 2— A group of 3 is in the shape of a false wheel under the ultimate bottle stem, the seat angle is 70-90°, the conidia are obova...

Embodiment 2

[0016] Trichoderma chrysogenum fungus strain TW351 ( Trichoderma aureoviride sp. ), the culture method of spores in mass production is the liquid-to-solid two-phase culture method, the conidia liquid is transferred into PD liquid medium, shaking culture for 2-4 days, and then according to the weight ratio of 1:6-10 with sterilized Mix evenly the rice bran or wheat bran of bacteria, put it in a stainless steel mesh sieve sterilized by lime water or ozone, cover it with a sterilized plastic film, place it in a room at 22-30°C for 3-5 days, open the plastic film, and produce spores 3-5 days; collect together with culture medium, dry, crush, pack and store.

Embodiment 3

[0018] Its concrete culture process description of Trichoderma chrysogenum fungus strain TW351:

[0019] Test tube strain culture: Inoculate the Trichoderma on PDA medium, and culture it in a 28°C incubator for 4-5 days to grow conidia;

[0020] Erlenmeyer flask liquid expansion culture:

[0021] Add 250ml of PD medium into a 1000ml Erlenmeyer flask, inoculate Trichoderma into a shaker, turn at 100-120, and culture at 28°C for 72 hours.

[0022] Seed tank submerged fermentation culture:

[0023] Wheat bran 10-20kg, cornmeal 10-20kg, sucrose 4-9kg, peptone 250-500g. Boil for 30-40 minutes and filter for later use. Urea 300-500g, ferrous sulfate 2-10g, potassium chloride 88-135g, sodium nitrate 15-32g, potassium dihydrogen phosphate 125-155g, add the above filtrate such as wheat bran, mix well, add water to make up to 200kg. Sterilize, cool to 28 degrees to inoculate Trichoderma, cultivate for 72-96 hours, and use to inoculate solid culture.

[0024] Solid fermentation cult...

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Abstract

The invention belongs to the field of microorganisms and microorganism application, and in particular relates to a trichoderma aureoviride strain TW351 (Trichodermaaureoviridesp.) and application of the trichoderma aureoviride strain in inhibition against plant diseases. The strain is stored in China Center for Type Culture Collection in Oct., 17th in 2012, and the preservation number is CCTCC NO:M2012407; the strain can quickly grow on a personal digital assistant (PDA) solid culture medium under the temperature of 28 DEG C; after 48 hours, the radius of a bacterial colony is 3.4 cm; after 72 hours, the radius of the bacterial colony is 5.4 cm; the form of the strain under an optical microscope is that formed mycelium is white or colorless and is in a short fur type; after being matured, the bacterial colony is light yellow green. When ginger seeds are planted, the seed gingers are soaked in a trichoderma preparation according to the proportion of the preparation to water of 1:100; and therefore, the ginger damping off disease prevention rate is up to 82 percent. When the damping off diseases are prevented, effects for accelerating germination of the ginger seeds and hestening the rooting of the ginger seeds can be achieved.

Description

technical field [0001] The invention belongs to the field of microbes and microbe applications, in particular to a Trichoderma chrysanthemum fungus strain TW351 ( Trichoderma aureoviride sp. ) and its application in the suppression of plant diseases. Background technique [0002] Due to the increasingly serious pollution of the environment by chemical pesticides, people are increasingly calling for sustainable agriculture. To solve this problem, it is very effective to use biological pesticides to prevent and control plant diseases and insect pests. In recent years, with the growing resistance of plant diseases and insect pests, the use of chemical pesticides has doubled, and my country's agricultural ecological environment and resources have been greatly damaged. The sustainable development of agriculture has become a top priority. Due to the disadvantages of chemical pesticides, the biopesticide market has begun to grow, and the concept of integrated pest management (IP...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
Inventor 王未名陈相艳陈建爱陈蕾蕾裘纪莹刘孝永孙欣周庆新祝清俊
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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