65 results about "Thioredoxin reductase" patented technology

Antisense oligomers directed to bacterial cell division and cell cycle-encoding nucleic acids are capable of selectively modulating the biological activity thereof, and are useful in treatment and prevention of bacterial infection. The antisense oligomers are substantially uncharged, and contain from 8 to 40 nucleotide subunits, including a targeting nucleic acid sequence at least 10 nucleotides in length which is effective to hybridize to (i) a bacterial tRNA or (ii) a target sequence, containing a translational start codon, within a bacterial nucleic acid which encodes a protein associated with cell division or the cell cycle. Such proteins include zipA, sulA, secA, dicA, dicB, dicC, dicF, ftsA, ftsI, ftsN, ftsK, ftsL, ftsQ, ftsW, ftsZ, murC, murD, murE, murF, murG, minC, minD, minE, mraY, mraW, mraZ, seqA, ddlB, carbamate kinase, D-ala D-ala ligase, topoisomerase, alkyl hydroperoxide reductase, thioredoxin reductase, dihydrofolate reductase, and cell wall enzyme.

The present invention provides novel emulsion formulations which comprise oil bodies. The invention also provides a method for preparing the emulsions and the use of the emulsions in a variety of products including food products, personal care products and pharmaceutical products. In a preferred embodiment the emulsions comprise thioredoxin and / or thioredoxin reductase.

The invention discloses a novel antitumor medicament, in particular a benzisoselenazolone difluorocytidine compound or a pharmaceutically acceptable salt or ester thereof as well as a preparation method and application thereof, wherein a benzisoselenazolone structure can identify selenocysteine residues in the activity center of thioredoxin reductase to generate the action of reversibly competitive inhibition and reserves the activity structure of 2'-deoxidization-2'2'-difluorocytidine. Therefore, the benzisoselenazolone difluorocytidine compound in the invention can selectively kill tumor cells, inhibit the growth of the tumor cells, and has the characteristics of high efficiency and low toxicity.

Provided herein is a method of treating cancer by the administration of binuclear gold(I) compounds in patients in need thereof. The pharmaceutical compounds possess anti-cancer activity such as the induction of cell death, inhibition of cellular proliferation, inhibition of thioredoxin reductase activity, and inhibition of tumor growth in vivo.

The present invention relates to a method for investigating body fluids for cancer cells, the use thereof and corresponding analysis kits, and the possibilities for cancer treatment derived therefrom. The method is based essentially on determining the expression of the manganese superoxide dismutase, thioredoxin reductase and / or glutathione peroxidase genes. Use of this method permits in particular reliable tumor diagnosis and prognosis. Diminishing an elevated expression of these genes has therapeutic value and may be utilized for cancer treatment.

Provided herein is a method of synthesis of Au(III)-NHC complexes, a pharmaceutical composition comprises thereof. Also provided herein are the methods for the treatment and prevention of cancer / tumor in patients in need thereof by the administration of the Au(III)-NHC complexes. Also provided is method of detecting thiol in a biological system. The Au(III)-NHC complexes possess anticancer activity such as the induction of cell death, inhibition of cellular proliferation, inhibition of thioredoxin reductase activity, and inhibition of tumor growth in vivo.

The mechanism of action of Ebselen differentiates between bacterial and mammalian thioredoxin reductase (TrxR). It displays fast oxidation of mammalian Trx and via the NADPH-TrxR catalyzed turnover of ebselen selenol with hydrogen peroxide, and therefore are mammalian antioxidants. Ebselen, and its diselenide, are strong competitive inhibitors of E. coli TrxR with Ki of 0.14 μM and 0.46 μM, respectively. E. coli mutants lacking glutathione reductase or glutathione were much more sensitive to inhibition by ebselen. Since either glutaredoxin or thioredoxin systems are electron donors to ribonucleotide reductase, ebselen targets primarily glutathione and glutaredoxin-negative bacteria, a class which includes major pathogens. Ebselen, and similar compounds are therefore useful as antibacterial agents, even for multiresistant strains. Two major pathogenic bacteria, which previously had not been known to be sensitive to ebselen, Mycobacterium tuberculosis (tuberculosis) and Helicobacter pylori (stomach ulcer and cancer), were shown to be excellent targets. Helicobacter pylori was also sensitive to ebsulfur.

The invention discloses an organoarsenic compound. The organoarsenic compound has the following structural formula as shown in the specification, wherein n is 1 or 2; R, R1, R2, R3, R4 and R5 are respectively and independently amino, R6-CO-NH, R7-CO-O, alkyl, aryl, a heterocyclic substituent, alkoxyl, alkylamino, a halogen atom, nitro, hydroxyl or a hydrogen atom; R6 is alkyl, aminoalkyl or aryl;R7 is alkyl, aminoaryl or aryl. The organoarsenic compound has good inhibitory activity for thioredoxin reductase, has strong cytotoxicity for tumor cell HL-60, and can be used for preparing a thioredoxin reductase inhibitor or an antitumor drug with thioredoxin reductase as a target spot.

The invention relates to a detection method used for predicting the occurrence risk of abnormal proliferation or tumors of the human body. The detection method comprises the following steps: 1) directly measuring the total mercapto content in a sample with dithio-bis-nitrobenzoic acid (DTNB); 2) using a specific thioredoxin reductase inhibitor compound to selectively inhibit the activity of thioredoxin reductase in the sample, and then, measuring the content of the other mercapto substances in the sample with DTNB, wherein the specific thioredoxin reductase inhibitor compound is the selen compound with TrxR inhibitory activity; and 3) subtracting the content of the other mercapto substances from the total mercapto content to obtain the content and activity of TrxR in the sample. The detection result in the invention can be used for predicting the occurrence risk of abnormal proliferation or tumors, and the method has specificity and high selectivity.

Provided is a direct method for detecting thioredoxin reductase (TR) activity in test samples. The method can provide a continuous and real-time measurement of TR activity. The method comprises contacting the test sample with NADPH and a diselenide substrate of TR, and then measuring conversion of NADPH to NADP. Also provided are kits for use in the method of direct detection of TR activity.

Antisense oligomers directed to bacterial cell division and cell cycle-encoding nucleic acids are capable of selectively modulating the biological activity thereof, and are useful in treatment and prevention of bacterial infection. The antisense oligomers are substantially uncharged, and contain from 8 to 40 nucleotide subunits, including a targeting nucleic acid sequence at least 10 nucleotides in length which is effective to hybridize to (i) a bacterial tRNA or (ii) a target sequence, containing a translational start codon, within a bacterial nucleic acid which encodes a protein associated with cell division or the cell cycle. Such proteins include zipA, sulA, secA, dicA, dicB, diCc, dicF, ftsA, ftsl,ftsN, ftsK, ftsL, ftsQ, ftsw, ftsZ, murC, murD, murE, murF, murg, minC, minD, minE, mraY, mraW, mraZ, seqA, ddlB, carbamate kinase, D-ala D-ala ligase, topoisomerase, alkyl hydroperoxide reductase, thioredoxin reductase, dihydrofolate reductase, and cell wall enzyme.

Activation of HIV-1 replication causes oxidative stress, which in turn potentiates HIV-1 replication. The common basis for the compounds of the present invention is: A) the capacity of reactivating HIV-1 from latency, and B) the ability to counteract the cellular machinery which activates in order to limit the effects of oxidative stress. In this way, oxidative stress can be potentiated and a “chain reaction” is sparked. This “chain reaction” induces a more efficient reactivation of HIV-1 from latency and, in some cases, induces selective killing of the infected cells. Actions A) and B) can either be carried out by one drug exerting both effects, or obtained by the combined use of distinct drugs. There are two main cellular machineries counteracting oxidative stress, i.e. the thioredoxin (Trx) thioredoxin reductase (TrxR) system and glutathione. Herein, we present drug strategies capable of exerting action B) by blocking either of the two machineries.

The invention belongs to the field of immunodiagnosis and discloses an aspergillus immunodominance antigen and an application thereof. The antigen is one or more of aspergillus thioredoxin reductase, fructose-bisphosphate aldolase, actin cytoskeleton protein V1P1 and secretory dipeptidyl peptidase. The aspergillus immunodominance antigen can react with specific antibodies in the serums of the patients suffering from invasive aspergillosis strongly and can be used for preparing a diagnostic reagent of invasive aspergillosis.

The invention discloses a synergistic detection device for thioredoxin reductase activity. The synergistic detection device comprises a detection machine, a detection cavity is arranged in the detection machine, a detection mechanism supporting experiments is arranged in the detection cavity, a driving mechanism is arranged in the lower end wall of the detection cavity to provide power for the operation of he device and includes a driving cavity, a motor is arranged on in the lower end wall of the driving cavity, a cleaning mechanism capable of cleaning and drying carriers is further arranged in the detection machine, and an adding mechanism for adding required reagents or detection samples to the carriers is further arranged in the upper end wall of the detection cavity. The device can automatically complete the addition of the samples or reagents when detection is needed, manual addition is not needed, the interference of other factors to the detection is reduced, the accuracy of the detection is improved, a culture dish is cleaned and dried at the beginning and end of the detection to ensure the cleanliness of the culture dish and less influence on the detection, and through mechanical transmission, the efficiency of the detection is improved.

The invention relates to an enzyme extraction and purification technology, specifically to a method of extracting and purifying thioredoxin reductase from chicken livers. The method comprises steps of obtaining a crude extract from fresh chicken livers; salting out; and extracting and purifying thioredoxin reductase from chicken livers by employing chromatography that uses two chromatography media comprising a DEAE anion exchange chromatography column and an ADP affinity chromatography column. The method provided by the invention is advantageous in that the raw material chicken livers have wide source and low cost; process of the extraction and purification technology is simple, and can be operated conveniently; finished products have high purity and the same capability of catalyzing human Trx reduction as cattle or mouse thioredoxin reductase, and therefore the finished products can be applied to scientific researches and practices, in place of cattle or mouse thioredoxin reductase, for human-related disease researches; and problems, of extraction and purification processes for cattle or mouse TrxR, comprising difficulty in obtaining raw materials, large individual differences, etc. are solved. The method provided by the invention fills in the blank of biological reagent preparations in our country and has high economic value.

The invention discloses collaborative detection equipment and a collaborative detection method of thioredoxin reductase activity, and belongs to the technical field of tumor detection. The detection equipment comprises an accommodating device, a reaction device, a sampling device and a driving system, wherein the accommodating device is used for accommodating samples and reagents and surrounding the axis to perform periodic rotation under the driving effect of the driving system; the reaction device is used for accommodating a reaction cup and surrounding the axis to perform periodic rotationunder the driving effect of the driving system; the sampling device is used for performing periodic rotation based on the accommodating device and the reaction device and collecting the reagents or the samples into the reaction device from the accommodating device; and the driving device is connected with the accommodating device, the reaction device and the sampling device separately and is usedfor controlling the accommodating device, the reaction device and the sampling device to act.

The invention discloses phenyl allylidene cyclohexenone derivatives with the structure shown in general formula I. In combination with structural characteristics and structure-activity relationship ofpiplartine, amino bonds are replaced with double bonds, meanwhile, active groups are reserved, aromatic substituent groups are changed, novel phenyl allylidene cyclohexenone derivatives targeting TrxR (thioredoxin reductase) for tumor tissue overexpression are designed, the defects of multiple piplartine synthesis steps and requirement for expensive metal catalysts are overcome, and the anti-tumor activity of the compounds is improved. Research results indicate that the compounds have strong inhabitation effects on proliferation of multiple tumor cells and can significantly increase the ROS level in the tumor cells and enhance the anti-tumor effect.

The invention discloses a triazole gold compound as well as a preparation method and application thereof. The structural general formula of the compound is shown as the description, wherein R1 is phenyl, p-methylphenyl, p-Chlorophenyl, a methoxyl group, methoxycarbonyl or p-fluorophenyl; R2 is triphenylphosphine, dimethyl -phenyl phosphine, 35 fluorine Phenyl phosphine or triethylphosphine; R3 is hydrogen, methyl or phenyl; R4 is hydrogen, methyl or phenyl; X is boron tetrafluoride negative ions or trifluoromethyl sulfonic acid negative ions. The inhibitory activity IC50 of the triazole gold compound disclosed by the invention (in nanomoles) indicates that the triazole gold compound is strongly combined with thioredoxin reductase active centers. The triazole gold compound disclosed by the invention has high anticellularity reproductive activity, is a thioredoxin reductase inhibitor adopting a novel structure, is good in physico-chemical property, is good in water solubility, and can be utilized as a lead compound of antitumor drugs.

Methods and reagent kits for determining the activity of thioredoxin reductase in a sample and the uses thereof are disclosed. The methods comprise determining the sulphydryl content of selen-compounds before and after treating the sample individually, consequently counting the activity of thioredoxin reductase in the sample by the difference of sulphydryl content before and after treating the sample. Additionally, the present invention also discloses the methods for evaluating the level of disease resistance in vivo, which comprise the step of determining the activity of thioredoxin reductase in a sample, and the step of counting the level of oxidative stress and the activity of thioredoxin reductase.

The invention provides a method for preparing fusion protein capable of lowering food allergen reaction, comprising the following steps: obtaining a thioredoxin gene Trx and a thioredoxin reductase gene TrxR in a thioredoxin system; designing a fusion peptide sequence; fusing the genes Trx and TrxR by the fusion peptide sequence; then, obtaining a recombination strain containing the fusion gene; and finally, carrying out heterology expression purification. The invention colons the genes of two proteins in an AnTrx system with wide application meaning in the industry and fuses and improves thegenes of Trx and TrxR, and a disulfide bond in the food protein is reduced by incubating with the food protein so as to lower the allergen reaction thereof. Meanwhile, improvement by gene fusion causes An-TrxR and An-Trx to be convenient to apply in the food industry when An-TrxR and An-Trx serve as a food additive.

The invention discloses a method for in-vitro evaluation on safety of cigarettes and tobaccos. The method is characterized in that a biological reagent with TrxR (thioredoxin reductase) activity is adopted to detect TrxR activity inhibition ability of CSE (cigarette smoke extract) obtained after combustion of the cigarettes or the tobaccos so as to evaluate safety of the cigarettes and the tobaccos by taking an absorbance value of the CSE at 340nm as a quantification basis. By the method for in-vitro evaluation on safety of the cigarettes and the tobaccos, the problems of long evaluation period, complex evaluation mechanisms and the like in current cigarette harm evaluation are solved successfully to provide a reliable evaluation means for enterprises to improve tobacco quality and develop less harmful cigarettes.

The invention belongs to the technical field of biomedicine and relates to kluyveromyces marxianus-derived alkyl peroxide reductase and thioredoxin reductase and an application thereof in improving stress resistance. The KmTPX1 gene of alkyl peroxide reductase gene contains 594 nucleotides, and 197 amino acids are coded; and the KmTrxR gene contains 960 nucleotides, and 319 amino acids are coded. Meanwhile, the KmTPX1 gene and KmTrxR gene can improve the tolerance of yeast cells against multiple inhibitors or stress factors, such as one or more of hydrogen peroxide, formic acid, acetic acid, furfural, ethanol, sodium chloride, phenol and guaiacol. Due to the improvement of the tolerance against formic acid, acetic acid, furfural, ethanol and salt ion concentration, the alkyl peroxide reductase gene and thioredoxin reductase gene play a crucial role in constructing a good cellulosic ethanol fermentation strain.

The invention provides an application of a detection reagent in preparation of drugs for evaluation of clinical tumor patient clinical treatment monitoring; the detection reagent includes the components respectively independently existing: (a) a reagent determining the total sulfydryl content in a sample; and (b) a thioredoxin reductase specific inhibition compound. Clinical treatment includes all kinds of clinical chemotherapies, radiation therapies or other clinical treatment schemes. The detection reagent has obvious activity difference on different treatment stages (such as preoperation, intraoperation and postoperation) and clinical effects (CR, PR, SD and PD) of lung cancer, breast cancer, nasopharyngeal carcinoma and cervical carcinoma, and data analysis display has a statistical significance, therefore, the detection reagent determines the tumor treatment effect through TR activity detection and dynamically monitors the process of related diseases, so that the detection reagent provides great guiding significance on different treatment stages of patients and can be used in clinic.

The invention discloses a cooperative detection device and method for thioredoxin reductase activity, belonging to the technical field of tumor detection. Among them, the detection equipment includes: a holding device, used to hold samples and reagents, and under the driving action of the driving system, periodically rotate around the axis; a reaction device, used to hold the cuvette, and Next, it rotates periodically around the axis; the sampling device is used to collect reagents and / or samples from the containing device and put them into the reaction device based on the periodic rotation of the containing device and the reaction device; the driving system is respectively connected with the containing device and the reaction device. The device is connected with the sampling device, and is used to control the actions of the containing device, the reaction device and the sampling device.

The invention discloses one or more immunodominant antigens of aspergillus selected from aspergillus thioredoxin reductase, fructose-bisphosphate aldolase, actin cytoskeleton protein VIP1, secreted dipeptidyl peptidase. The invention further discloses a use of the antigen for preparing diagnostic reagents for invasive aspergillosis, and diagnostic kits for invasive aspergillosis comprising the antigen.