58 results about "Streptomyces phaeochromogenus" patented technology

A marine streptomyces viridochromogenes strain for producing alkali-tolerant and salt-tolerant xylanase and application of the marine streptomyces viridochromogenes strain belong to the field of biotechnology. The strain is collected in the China General Microbiological Culture Collection Center, and the collection number is 5565. The strain can normally grow under the extreme alkaline environment of pH11, shows a good oligotrophy-tolerant characteristic, can degrade a variety of hemicellulose-containing biomasses including corn straws, rice straws, Jerusalem artichoke straws, kelp fibers and the like, and has a broad application prospect in utilizing cheap biomass resources to produce bio-based chemicals. The strain can produce xylanase under optimum conditions, and the enzymatic activity of the xylanase can be as high as 68.9U / ml. The produced xylanase has the following properties: the optimum pH value is 6.0, the optimum temperature is 70 DEG C, the optimum substrate is beech xylan, and the produced xylanase does not have cellulase activity, has high pH stability and thermal stability, and can tolerate high-concentration NaCl. As an enzyme preparation coming from the ocean, the xylanase can be widely used in papermaking, food, feed, wine making, energy and other industries.

The invention provides a method for improving the output of nikkomycin. The invention specifically provides a fermentation medium for Streptomyces ansochromogenes and a method for preparing nikkomycin by using the medium. According to the invention, a proper amount of Tween 80 is added into the fermentation medium to further optimize the varieties and amounts of specific components of the fermentation medium and other culture conditions are controlled, so the output of nikkomycin is increased; the output of nikkomycin in the invention is as high as 42600 [mu]g / mL; and the method is simple, low in cost and suitable for industrial large-scale production.

The present invention provides streptomyces erythrochromogenes MO28, which has a preservation number of CGMCC No.3228. The strain is capable of inhibiting the growth of tomato gray mold. Results of panel antibacterial tests show that MO28 strain has high inhibition effect on various plant pathogenic fungi and plant pathogenic bacteria, wherein the fusarium moniliforme inhibition capacity is the highest and the fusarium moniliforme inhibition rate is up to 69.9 percent; and the pseudomonas syringae inhibition diameter is 53.7millimeter. The tomato fruit gray mold prevention and control efficiency of the MO28 is 96.56 percent and the tomato leaf gray mold prevention and control efficiency of the MO28 is 78.26 percent. The strain has no obvious difference from 28-percent chlorothalonil and diethofencarb wettable powder. Results of greenhouse prevention effect detection show that the efficiency of the prevention and control of gray mold on tomatoes, pepper, cucumbers, egg plants and strawberry of the MO28 is between 63 and 77 percent.

The invention relates to a screening method for a high-yielding strain of polyoxin I and solves the technical problem of lower content of polyoxin I in an existing strain. An original strain is Streptomyces ansochromogenes; the method comprises steps as follows: a spore suspension of the original strain is prepared, a 5-fluorouracil-containing resistant plate is coated with the diluted spore suspension subjected to ultraviolet mutagenesis, and after culture, resistant strains are screened and the high-yielding strain of polyoxin I is obtained. Fermentation broth of the screened high-yielding strain of polyoxin I has an inhibition effect on alternaria alternata. The high-yielding strain can be rapidly screened out with the screening method.

The invention discloses a cellulose degrading bacterium and application thereof, and relates to a cellulose degrading microorganism and application thereof. According to the present invention, the cellulose degrading bacterium is Streptomyces ansochromogenes WF-10, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.23720; the invention also discloses an application of the cellulose degrading bacterium in cellulose biodegradation. The strain disclosed by the invention can grow on a CMC (Carboxymethyl Cellulose) plate taking sodium carboxymethyl cellulose as a unique carbon source, and an obvious cellulose decomposition transparent ring appears around a bacterial colony after the bacterial colony is dyed by Congo red; according to the invention, the cellulase can secrete four types of cellulase, including filter paper enzyme, endo-beta-1, 4-glucanase, exo-beta-1, 4-glucanase and beta-glucosidase; the capability of degrading natural cellulose is realized.

The invention discloses recombinant streptomyces ansochromogenes, a preparation method thereof and use thereof. The recombinant streptomyces ansochromogenes are recombinant bacteria obtained by introducing nikkomycin biologic synthesis gene clusters into the streptomyces ansochromogenes, wherein the nikkomycin biologic synthesis gene clusters can be introduced into the streptomyces ansochromogenes by recombinant plasmids pNIK. Compared with the streptomyces ansochromogene strain 7100 CGMCC4.321, the recombinant streptomyces ansochromogenes of the invention can improve the nikkomycin Z yield by about 1.8 times and the nikkomycin X yield by about 3 times. The recombinant streptomyces ansochromogenes obviously improve the yield of the nikkomycins and provides necessary technical preparation for the large-scale production of the nikkomycins.