32 results about "Rheum tanguticum" patented technology

The invention discloses a pharmaceutical composition with notable curative effect and without drug resistance for treating tuberculosis, composed of croton, rhubarb and rhizoma bletillae, wherein, the croton is the dried ripening fruits of the euphorbiaceae family plant croton, the rhubarb is the dried root and rhizome of the polygonaceae family plant rheum palmatum L, rheum tanguticum or rheum officnale baill, the rhizoma bletillae is the dried tuber of the arethusa plant rhizoma bletillae, and the croton needs to be processed for application. The pharmaceutical composition is composed of the following raw materials in proportion by weight: 0.6-1.2 of croton (processed), 10-40 of rhubarb and 0-30 of rhizoma bletillae. The pharmaceutical composition for treating tuberculosis disclosed by the invention has the advantages of exact curative effect, little side effect, no drug resistance and the like, and shown by pharmacological experiments and clinical data, the pharmaceutical composition preparation has low toxic side effect and notable curative effect.

The invention discloses a thin-layer chromatography detection method of rheum palmatum as a traditional Chinese medicinal material. The rheum palmatum is detected with thin-layer chromatography. The method comprises steps as follows: (1) preparation of a test liquid: taking test medicinal material powder, adding ethanol for extraction, performing filtering and concentrating, adding water for dissolution, adding an organic solvent for extraction, performing concentration, adding methanol to constant volume, and taking the solution as the test solution; (2) thin-layer chromatography detection: sampling the test solution on a thin-layer plate, performing developing with petroleum ether at 30-60 DEG C, n-hexane, ethyl acetate and formic acid as developers, and performing color development, inspection and contrast. According to the thin-layer chromatography detection method, purple characteristic spots are found in the Rf value range of 0.13-0.17 and are unique to the rheum palmatum, the spots are clear and easy to distinguish, and the rheum palmatum can be effectively distinguished from rheum palmatum, rheum tanguticum and rheum tanguticum.

The invention relates to a rheum tanguticum Maximin producing area processing and processing production integrated process which comprises the following steps: (1) treating fresh rheum tanguticum Maximin: removing impurities from fresh rheum tanguticum Maximin medicinal material completely, and scraping off skin; naturally airing until the water content is 50-60% of the water content of the raw medicinal materials or drying at a constant temperature of 40-60 DEG C until the water content is 50-60% of the water content of the raw medicinal materials; (2) cutting: cutting the treated fresh rheumtanguticum Maximin medicinal material into cubes of 8-12mm; and (3) drying: naturally airing or drying the cut rheum tanguticum Maximin fresh medicinal material. The preferable rheum tanguticum Maximin producing area processing and processing production integrated process is stable, reasonable, feasible and high in operability, can be used for guiding actual production, not only can greatly reduce enterprise cost, improve production efficiency and realize efficient utilization of medicinal material resources, but also can effectively avoid loss of main effective components. Decoction pieces with excellent quality are produced and scientific basis is provided for standardized production, popularization and application of rheum officinale decoction pieces in production places.

The invention relates to an antibacterial and hemostasis toothpaste composition and a preparation method thereof. The toothpaste composition comprises components by weight as follows: 25%-30% of bushcinquefoil, 35%-40% of rheum tanguticum, 3%-5% of sorbitol, 4%-5% of glycerin, 13%-20% of hydrated silica, 2%-3% of sodium lauryl sulfate, 2%-3% of xanthan gum and 0.5%-1% of essence. The preparationmethod of the composition comprises the steps as follows: clean bush cinquefoil lbranches and leaves are crushed, and ultrasonic extraction, filtration and acidity adjustment are performed to obtain extract; extraction and concentration are performed on clean rheum tanguticum roots to obtain rheum tanguticum extract; two extracts are uniformly mixed, a distilled water mixture of hydrated silica and xanthan gum with water is added, and stirring is performed; glycerin and sorbitol are added, and the mixture is uniformly mixed; and after the mixture is placed for 24 h, the sodium lauryl sulfate,mentol and sodium saccharin are added, and stirring, check, extract storage and canning are performed. The antibacterial and hemostasis effects of toothpaste are improved, and a solvent used during preparation is ethanol, thereby avoiding pollution.

The invention discloses a processing method of rheum tanguticum. The processing method comprises the following steps: material preparing: selecting a fresh rheum tanguticum product, removing rough skin on the surface of the fresh rheum tanguticum product, and cutting off branch roots; slicing: cutting the fresh rheum tanguticum into slices by using a stainless steel knife; and primary baking: feeding the sliced rheum tanguticum into an oven, and starting a baking mode to continuously bake the rheum tanguticum at a constant temperature. According to the invention, a three-time baking and cooling mode is set, so that the problems that the existing rheum tanguticum processing method wastes time and labor and occupies space, long-term exposure in air easily causes mould growth, and the influence on the appearance property and the internal quality of the medicinal materials is great are solved; the processing method of rheum tanguticum has the advantage of short processing time, can save time and manpower, is high in drying efficiency, relatively low in processing cost, simple and feasible in processing method and applicable to large-scale processing, and is worthy of popularization andapplication, wherein the medicinal material properties and the chemical component content of rheum tanguticum meet pharmacopeia standards.

The natural plant specific medicine for curing AIDS is made of at least five Chinese medicinal materials selected from rheum tanguticum, vitis amurensis, inula cappa, pteris vittata, kalimeris indica, scutellaria bartate, bidens pilosa, hedyotis diffusa, carthamus and cephalotaxus fortunei and adding excipient. The oral medication of said specific medicine can be matched with massage therapy in the mode of large circle of vital energy and cleaning skin with medicated lotion so as to attain the goal of discharging AIDS virus from interior of human body and eliminating AIDS virus from skin surface of human body to cure AIDS.

The invention discloses a five-year-old Rheum tanguticum organic cultivation method, and belongs to the technical field of medicinal plant artificial cultivation. The organic cultivation method includes selecting a plateau alpine wet climatic environmental condition; selecting high altitude wild seeds; performing seedling raising, or directly sowing the seeds; using a plastic film to retain heat, moisture and remove weed in the first two years; mechanically removing weed later; applying an organic fertilizer without farm chemical; enclosing soil and discharging water; removing leaves and sprouts; testing samples after five years; digging the Rheum tanguticum if the test is qualified; and performing numbering and recording to achieve quality tracing. The obtained Rheum tanguticum is high in yield, is good in quality, is low in labor cost, can provide qualified Rheum tanguticum for pharmaceutical enterprises, and can improve the economic income and the planting positivity of farmers.

The invention belongs to the technical field of biopesticide, and particularly relates to a recipe and a preparation process of a rhubarb free anthraquinone nanometer emulsion preparation, and application of the rhubarb free anthraquinone nanometer emulsion preparation to the prevention and the treatment of the plant red spider disease. The rhubarb free anthraquinone is extracted from traditional Chinese medicine sorrel, rheum tanguticum or rheum officinale. Through being metered by the mass percentage of each ingredient contained in the nanometer emulsion, the content of the rhubarb free anthraquinone in the nanometer emulsion accounts for 0.05 to 0.16 percent; the oil phase in the nanometer emulsion accounts for 18 to 22 percent; the emulsifiers account for 16 to 20 percent; co-emulsifiers account for 4 to 6 percent; water accounts for 50 to 61 percent; stabilizing agents account for 1.0 percent; preservatives account for 0.1 percent; attaching agents account for 0.1 percent. The rhubarb free anthraquinone nanometer emulsion preparation can be used for preventing and treating the plant red spider disease. The free anthraquinone from the rhubarb free anthraquinone nanometer emulsion preparation disclosed by the invention is from the traditional chinese medicine rhubarb; the applicability of the extraction process is high; the raw material obtaining cost is low; the nanometer emulsion preparation is automatically formed; the process is simple; the quality is controllable; the mass production can be easily realized; excellent prevention and treatment effects are achieved on the plant red spider disease.

The invention discloses a composite pig feed for enhancing immunity and promoting growth. The preparation method is characterized by comprising the following steps: weighing 3-10 parts by weight of damnacanthus root, 3-10 parts by weight of phoenix fruit, 2-6 parts by weight of Akebia Fruit, 2-6 parts by weight of Desmodium sambuense root, 2-6 parts by weight of Potentilla glabra, 1-3 parts by weight of Begonia fimbristipula, 1-3 parts by weight of rosebay leaf, 1-3 parts by weight of dicliptera, 1-3 parts by weight of Rheum tanguticum, 10-20 parts by weight of corn flour, 40-80 parts by weight of wheat bran and 50-90 parts by weight of rice bran; and mixing, pulverizing into fine powder capable of passing through a 100-mesh screen. The composite pig feed has the functions of enhancing disease resistance of pigs, increasing the growth speed and improving the flesh texture, has the advantages of high safety coefficient and no negative influence on human health.

The present invention provides a method for extracting a DNA from Rheum tanguticum Maxim. The method includes the following steps: after being soaked in distilled water for 24 hours, the dry Rheum tanguticum Maxim is cut into small pieces, dried and then ground into powder; the medicine powder is extracted by extraction medium I and Beta-mercaptoethanol and then by extraction medium II and Beta-mercaptoethanol; after the product is extracted by chloroform / isoamylol, the aqueous phase is added into NaAc(pH5.2) and ice-cold isopropanol in order to precipitate DNA; the aqueous phase is discarded; the precipitate is washed by 75 percent of ethanol for two to three times and then naturally aired after the ethanol is removed; the DNA is dissolved in sterilizing ddH2O; and the solution is kept under the temperature of 4 DEG C or placed under the temperature of 20 DEG C below zero for a long time. Being tested by electrophoresis, the extracted Rheum tanguticum Maxim. DNA is an intact genome DNA, and the purity is high (A260 / 280 equal to 1.8 to 20). Serving as a template, the obtained medicine genome DNA is amplified respectively by UBC888 primer and UBC891 primer, so that a map with clear strips can be obtained.

The invention provides an application of petiole from rheum tanguticum maxim in preparation of health foods or medicines. The invention particularly provides an application of petiole from rheum tanguticum maxim in preparation of antioxidant health-care foods and an application of petiole from rheum tanguticum maxim in preparation of drugs for resisting oxidation, reducing uric acid and clearing away heat and toxic materials. Research finds that the petiole from rheum tanguticum maxim has good effects of resisting oxidation, reducing uric acid and clearing away heat and toxic materials, and can prevent and / or treat gout or diminish inflammation. Therefore, the petiole from rheum tanguticum maxim can be prepared into health-care foods or medicines with related efficacy. The invention also provides a preparation method of the rheum tanguticum maxim petiole juice. By adopting the method, the astringent taste of the petiole from rheum tanguticum maxim can be overcome, thereby obtaining rheum tanguticum maxim petiole juice with excellent taste. The method is simple and convenient in production process and easy to realize industrial production. According to the invention, the economic value of petiole from rheum tanguticum maxim is expanded, and the petiole from rheum tanguticum maxim has a good market prospect.

The invention relates to identification of three original species of a rhei radix et rhizoma medicinal material and an application of the identification. The identification specifically comprises the following steps: screening hypervariable regions based on chloroplast genomes, designing primers 1F / 1R, 2F / 2R, 3F / 3R, 4F / 4R and 5F / 5R by taking the screened hypervariable regions rps16-trnQ, psaA-ycf3, psbE-petL, ndhF-rpl32 and trnT-trnL sequences as templates, performing PCR amplification and Sanger sequencing on 42 samples, evaluating the species identification capability of the screened sequences by utilizing MEGA7.0, and determining specific DNA bar codes capable of being used for identifying dried roots and stems of rh. tanguticum maxim. ex balf., rh. officinale baill. and rheum palmatum L. The invention discloses five pairs of universal primers capable of effectively amplifying the hypervariable regions rps16-trnQ, psaA-ycf3, psbE-petL, ndhF-rpl32 and trnT-trnL, can be used for identifying original plants, seeds and medicinal materials of three basic original species of the rhubarb medicinal material, and provides a basis for classifying and identifying the three basic original species of the rhubarb medicinal material, ensuring the clinical medication safety of the rhei radix et rhizoma medicinal material and standardizing the rhei radix et rhizoma medicinal material market.

The invention provides a no-tillage ecological tending method for rheum tanguticum Maxim.ex Balf.. The method includes steps of seed selection, tending zone selection, sowing, tending management and harvesting. The provided method can cultivate high-quality genuine rheum tanguticum Maxim.ex Balf. medicinal materials, and has characteristics of maximumly alleviating bad ecological environment impacts, saving time and labor and lowering costs, so that the sustainable development of China-Tibet medicinal material industries can be promoted, and therefore, the method has good popularization and application values.

The invention relates to a habitat processing and preparing production integrated technology for rheum tanguticum pieces. The habitat processing and preparing production integrated technology comprises the following steps of (1) treating fresh rheum tanguticum: removing impurities of fresh rheum tanguticum medicines, scrapping and removing outer covers, and performing natural drying until the water content is 50-60% of that of the raw medicines; (2) performing cutting: cutting the rheum tanguticum which is naturally dried into pieces being 8-10mm in thickness; and (3) performing drying: naturally drying or baking the rheum tanguticum pieces obtained by cutting. The habitat processing and preparing production integrated technology for rheum tanguticum pieces, which is preferably selected, is stable, reasonable, practical and high in operability, and can be used for guiding actual production. The business cost can be greatly reduced, the production efficiency can be improved, high-efficiency utilization of medicine material resources can be realized, loss of main effective components can also be effectively avoided, decoction pieces being good in quality can be produced, and a scientific basis can be provided for habitat standardized production and promotion application of the rheum tanguticum decoction pieces.

The invention discloses a rheum officinale tea beverage capable of reducing lipid and reducing weight. The rheum officinale tea beverage capable of reducing lipid and reducing weight comprises 500 to1000 parts of R. tanguticum Maxim. ex Balf. stem, 5 to 20 parts of mint, 10 to 30 parts of lotus plumule, 30 to 50 parts of tea, 20 to 30 parts of honeysuckle flower, and 5 to 10 parts of scorched cassia seed. The preparation method possesses following steps: A1, rinsing, wherein R. tanguticum Maxim. ex Balf. stem, mint, lotus plumule, tea, honeysuckle flower, and scorched cassia seed are immersedin purified water. According to the preparation method, R. tanguticum Maxim. ex Balf. stem, Platycodon grandiflorum, mint, lotus plumule, tea, honeysuckle flower, scorched cassia seed, and pericarpium citri reticulatae are smashed at a certain weight ratio, and the obtained drug material powders capable of reducing lipid are mixed, tea and honeysuckle flower are added for blending so as to obtainthe tea beverage for daily using. The preparation method is capable of solving a problem that the taste of conventional drug used for reducing lipid and weight is bitter, so that lipid lowering and weight reducing can be realized in daily life through drinking of tea.

The invention discloses a traditional Chinese medicine decoction for treating functional constipation and a preparation method for the traditional Chinese medicine decoction. The traditional Chinese medicine decoction is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20-30 parts of honey, 15-25 parts of rheum tanguticum, 10-20 parts of fructus cannabis, 10-20 parts of semen trichosanthis, 8-16 parts of Chinese wolfberry, 5-15 parts of fructus perillae, 5-15 parts of auricularia auricula, 5-15 parts of tremella, 3-13 parts of walnut kernel, 2-8 parts of folium sennae, 2-8 parts of cynomorium songaricum and 1-5 parts of castor bean. The traditional Chinese medicine decoction for treating functional constipation adopts common Chinese herbal medicines as the raw materials, is scientific in formula, better in taste, low in cost, comprehensive in treatment, obvious in curative effect, low in relapse possibility and free of side effect, has the efficacies of relaxing bowel, tonifying spleen and kidney, tonifying qin and blood, moistening dryness and ventilating a lung, and can be used for effectively treating the functional constipation.

The invention discloses a method for treating a black soil beach, which comprises the following steps: leveling a land, applying a base fertilizer, digging pits, selecting annual rheum tanguticum seedlings and elymus nutans seeds, transplanting the rheum tanguticum seedlings after the soil is unfrozen in spring every year, and planting one seedling in each pit. After the transplanting of the rheum tanguticum is completed, sowing elymus nutans seeds in the middle of the line spacing of the transplanting pits of the rheum tanguticum according to the sowing rate of 3kg / mu. Finally, urea is applied twice in July and August respectively. According to the method, the rheum tanguticum and the elymus nutans are intercropped, the rheum tanguticum in a simulated wild environment is used as an associated species to form a stable community with the alpine grassland, a deep root system is matched with a shallow root system, broad-leaved plants and gramineous plants are intercropped, community diversity can be increased, resources can be fully utilized, and grazing tolerance is good; and the problem of secondary degradation of the black soil beach is effectively solved. Through the intercropping of the herbs, the vegetation coverage of a planting area and the overground biomass are also greatly increased, so that the purpose of preventing and treating rodent damage is achieved.

The invention relates to the field of agricultural planting, in particular to a cultivation method of interplanting rheum tanguticum with yuenkanin. The method includes the steps of leveling the land, applying base fertilizers, plowing and breaking to rake; in accordance with the row spacing of 50-80 cm, ditching by depth of 2 cm and plant spacing of 25-50 cm to sow rheum tanguticum seeds; ditching in the middle of row spacing of rheum tanguticum by the depth of 1 cm, and sowing yuenkanin seeds according to plant spacing of 8-15 cm; after the two kinds of seeds germinate, conducting topdressing two times in July and August respectively. The rheum tanguticum and the yuenkanin grow together, wherein as a biennial plant, the yuankanin can be harvested in October of the planting year; with a rapid growth speed, the yuankanin can quickly occupy the intermediate space of the rheum tanguticum, thereby preventing weeds from spreading, and reducing the field weeding cost of the rheum tanguticum. According to the cultivation method of interplanting medicinal plants of rheum tanguticum with forage plants of yuenkanin, the large-scale industrialization plantation of the rheum tanguticum and yuenkanin can be achieved, thereby realizing double incomes, and achieving the purpose of adjusting planting structure for the vast Qinghai Tibet Plateau pastoral areas.

The invention discloses a pharmaceutical composition with notable curative effect and without drug resistance for treating tuberculosis, composed of croton, rhubarb and rhizoma bletillae, wherein, the croton is the dried ripening fruits of the euphorbiaceae family plant croton, the rhubarb is the dried root and rhizome of the polygonaceae family plant rheum palmatum L, rheum tanguticum or rheum officnale baill, the rhizoma bletillae is the dried tuber of the arethusa plant rhizoma bletillae, and the croton needs to be processed for application. The pharmaceutical composition is composed of the following raw materials in proportion by weight: 0.6-1.2 of croton (processed), 10-40 of rhubarb and 0-30 of rhizoma bletillae. The pharmaceutical composition for treating tuberculosis disclosed by the invention has the advantages of exact curative effect, little side effect, no drug resistance and the like, and shown by pharmacological experiments and clinical data, the pharmaceutical composition preparation has low toxic side effect and notable curative effect.

The invention relates to a rheum tanguticum chalcone synthase Rt-CHS1 gene, a protein and a cloning method and in particular relates to the technical field of biological gene cloning. By adopting a method that an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technique and an RACE (Rapid-Amplification of cDNA Ends) technique are combined, a rheum tanguticum Rt-CHS1 gene is studied, a cDNA complete nucleotide sequence of the Rt-CHS1 gene is obtained the cloning of rheum tanguticum roots for a first time, and amino acid sequences encoded by the gene are analyzed. A base is made for further study on expression and regulation on the rheum tanguticum Rt-CHS1 gene, reference is provided for study on synthesis and regulation mechanisms of the Rt-CHS1 gene with rheum tanguticum anthraquinones, and meanwhile new background information is provided for function and evolution study on a polygonaceae gene CHS1 gene. In addition, cloning of the rheum tanguticum Rt-CHS1 gene has great significances for increasing the content of anthraquinone compounds in rheum tanguticum and improving the quality of medicinal materials.

The invention discloses a method extracting sennoside A in rheum tanguticum maxim ex balf. The method comprises the following steps: weighing 0.25 g of rheum tanguticum maxim ex balf powder; dissolving the powder in 40.8 mL of NaHCO3 solution of which the mass percentage concentration is 0.15%; conducting supersonic extraction for 40.5 min; filtering. The method optimizes the extracting method of the sennoside A in the rheum tanguticum maxim ex balf for the first time, and is stable; the extracting rate of the sennoside A is high; the method lays foundation for purification and industrialized development of the sennoside A in the rheum tanguticum maxim ex balf.

The invention discloses a rheum tanguticum maxim liquid for dispelling the effects of alcohol. The rheum tanguticum maxim liquid is prepared from 500-1000 parts of stems of rheum tanguticum maxim, 10-30 parts of pueraria seed, 5-20 parts of Chinese olive, 10-30 parts of sweet orange peel, 30-50 parts of honey and 5-10 parts of mountain ginseng. A preparation method of the rheum tanguticum maxim liquid comprises squeezing of stems of rheum tanguticum maxim, decoction, sterilization, mountain ginseng extraction, mixing and packaging. The preparation method mixes and processes stems of rheum tanguticum maxim, pueraria seed, Chinese olive, sweet orange peel, honey, vinegar and mountain ginseng. The preparation method utilizes simple raw materials, realizes a low cost, is conducive to cost control, has simple processes and is conducive to batch processing production. The rheum tanguticum maxim liquid can be drunk after a meal to dispel the effects of alcohol, can protect the intestines andstomach through use before a meal and has alcohol dispelling effects better than those of the products currently on the market.

The invention discloses a preparation method of a beverage taking rheum tanguticum leaves as a main raw material. The preparation method comprises the following steps: A, material selecting: selectingraw materials required for preparing a beverage; B, cleaning: putting the selected solid raw materials into a cleaning box, and carrying out cleaning treatment; C, drying: putting the cleaned solid raw materials into a drying box, and carrying out drying treatment; D, impurity removal: putting the dried solid raw materials into an impurity removal box, and carrying out impurity removal treatment;and E, crushing: putting the solid raw materials subjected to impurity removal into a crusher, and carrying out crushing treatment. According to the invention, rheum tanguticum leaves are used as main raw materials, are rich in chrysophanol and emodin, have high nutritional value and health-care functions, and have the functions of invigorating the stomach, helping digestion, reducing fat, losingweight, wetting and relaxing the bowels, and the prepared beverage is suitable for most people to drink, and particularly has obvious effects of reducing fat and losing weight for obese people.

The invention discloses a thin-layer chromatography detection method of rheum palmatum as a traditional Chinese medicinal material. The rheum palmatum is detected with thin-layer chromatography. The method comprises steps as follows: (1) preparation of a test liquid: taking test medicinal material powder, adding ethanol for extraction, performing filtering and concentrating, adding water for dissolution, adding an organic solvent for extraction, performing concentration, adding methanol to constant volume, and taking the solution as the test solution; (2) thin-layer chromatography detection: sampling the test solution on a thin-layer plate, performing developing with petroleum ether at 30-60 DEG C, n-hexane, ethyl acetate and formic acid as developers, and performing color development, inspection and contrast. According to the thin-layer chromatography detection method, purple characteristic spots are found in the Rf value range of 0.13-0.17 and are unique to the rheum palmatum, the spots are clear and easy to distinguish, and the rheum palmatum can be effectively distinguished from rheum palmatum, rheum tanguticum and rheum tanguticum.

The invention discloses an external-use traditional Chinese medicine composition for treating perianal diseases. The external-use traditional Chinese medicine composition comprises crude medicines ofroots and tubers of cynanchum paniculatum (Bge.) Kitag., insect galls on leaves of rhuschinensis Mill. or rhuspotaninii Maxim., herbs of taraxacum mongolicum Hand. Mazz. with roots, leaves of callicarpa macrophylla Vahl., roots and tubers of rheum palmatum L., rheum tanguticum Maxim.ex Reg. or rheum officinale Baill., roots or vines of kadsura coccinea (Lem.) A.C.smith, and selectable medicines capable of clearing heat and reducing swelling, and / or medicines capable of clearing heat and relieving pain. The traditional Chinese medicine external-use composition disclosed by the invention has theefficacy of clearing heat, removing toxicity, reducing the swelling, relieving pain, promoting convergence, stopping bleeding and the like, and has good effects in the respects of treating partial gall and bleeding caused by common perianal diseases such as haemorrhoids, fistula, abscess and anal fissure, preventing infection, promoting wound healing and the like. The composition has the characteristics of being light in medicine taste, obvious in efficacy, low in price and wide in adaptation diseases; all the medicines in the prescription of the external-use traditional Chinese medicine composition can be orally taken, so that the external-use traditional Chinese medicine composition has small toxic and side effects, cannot cause any serious adverse reactions, and is especially suitablefor clinical application.

The invention relates to an efficient hybridization method for rheum tanguticum. The method includes the steps that a, parchment paper bags which are 6 cm wide and 15-25 cm long are sewn; b, parents are selected preferably, and a hybridization combination is configured, wherein male parent and female parent plants are selected preferably, and female parent spicas and buds are reserved selectively; c, hybridization time is determined; d, anther of the mature and fresh male parent plants is collected for use; e, female parent buds are screened, and bud operation is carried out, wherein female parent buds are handpicked, and then bud operation is carried out; f, pollination and hybridization are performed, wherein pollen is shaken off, and the anther is inserted; g, bagging is carried out; h, bags are removed in time; i, hybrid seeds are harvested at the right moment; j, true-false authentication is carried out. Rheum tanguticum flower hybridization operation can be efficiently carried out in quantity to obtain the hybrid seeds, excellent variation groups are created, and the effect is remarkable in seed breeding.

The invention belongs to the technical field of biopesticide, and particularly relates to a recipe and a preparation process of a rhubarb free anthraquinone nanometer emulsion preparation, and application of the rhubarb free anthraquinone nanometer emulsion preparation to the prevention and the treatment of the plant red spider disease. The rhubarb free anthraquinone is extracted from traditional Chinese medicine sorrel, rheum tanguticum or rheum officinale. Through being metered by the mass percentage of each ingredient contained in the nanometer emulsion, the content of the rhubarb free anthraquinone in the nanometer emulsion accounts for 0.05 to 0.16 percent; the oil phase in the nanometer emulsion accounts for 18 to 22 percent; the emulsifiers account for 16 to 20 percent; co-emulsifiers account for 4 to 6 percent; water accounts for 50 to 61 percent; stabilizing agents account for 1.0 percent; preservatives account for 0.1 percent; attaching agents account for 0.1 percent. The rhubarb free anthraquinone nanometer emulsion preparation can be used for preventing and treating the plant red spider disease. The free anthraquinone from the rhubarb free anthraquinone nanometer emulsion preparation disclosed by the invention is from the traditional chinese medicine rhubarb; the applicability of the extraction process is high; the raw material obtaining cost is low; the nanometer emulsion preparation is automatically formed; the process is simple; the quality is controllable; the mass production can be easily realized; excellent prevention and treatment effects are achieved on the plant red spider disease.

The invention relates to a medicine material specification dividing and production place processing method of Rheum tanguticum Maxim. ex Regel. The medicine material specification dividing and production place processing method comprises the following steps of: carrying out Rheum tanguticum Maxim. ex Regel medicine material specification classification, slicing, drying and the like. The rank division method to which the invention relates is simple and accurate, different ranks of medicine materials have an obvious quality and ingredient content difference, and the rank division method can be used for guiding enterprises to purchase different specifications of Rheum tanguticum Maxim. ex Regel on the market according to requirements. The Rheum tanguticum Maxim. ex Regel optimized by the invention has a simple and reasonable processing method and a clear parameter and is convenient in practical production, the content of the relevant medicinal components of the Rheum tanguticum Maxim. ex Regel can be effectively improved, and technical support is provided for normalized production of Rheum tanguticum Maxim. ex Regel medicine material decoction pieces.