How to Petunia hybrida?

The invention discloses separating and culturing methods for mesophyll protoplasts of petunia hybrida. The separating method for the protoplasts comprises following steps: tender leaves of the petunia hybrida are weighed, processed and subjected to enzymolysis with an enzymatic hydrolysate; a CPW (cell protoplast washing) medium is added to a solution obtained after enzymolysis, the solution is filtered and centrifuged, and a supernatant is absorbed; the CPW medium is added again for washing, centrifugation is performed, precipitates are collected, and the purified protoplasts are obtained. The culturing method for the protoplasts comprises steps as follows: the prepared protoplasts are added to a protoplast culture solution and are cultured for 30 d with a solid-liquid culture method in a dark environment at the temperature of 24 DEG C, and a miniature callus formed through culture is transferred to an enrichment medium; the miniature callus is cultured for 21 d and then is transferred to a differential medium; culturing is performed. With the adoption of the separating and culturing methods, the protoplasts with high activity and high yield can be obtained, the callus can be induced through culturing of the protoplasts, shoots can be differentiated finally, an experimental material is provided for study of further somatic cell fusion and the like of the petunia hybrida, and a foundation is laid for germplasm resource innovation of the petunia hybrida.

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Owner:SHANGHAI JIAO TONG UNIV

Herbaceous flower seed priming method

InactiveCN102415235ASufficient oxygenOvercome the disadvantage that it is not easy to separate the seedsSeed and root treatmentSeed primingPolyethylene glycol

The invention discloses a herbaceous flower seed priming method. The method comprises the following steps: 1) carefully selecting seeds; 2) preparing a polyethylene glycol solution with an osmotic potential being between -0.5 and -2.0 Mpa; 3) pouring a PEG solution into a container, putting a semi-permeable membrane with a same size on the solution, putting the seeds to be treated on the semi-permeable membrane, allowing the container to stand at 10 DEG C-20 DEG C for 7-14 days; 4) drying at 25 DEG C for 24 hours, or directly sowing without drying. The invention adopts a semi-permeable membrane to control the water absorption process of the seeds, avoids direct contact of the seeds with the solution, is applicable to the priming treatment of high-value herbaceous flower seeds, and is especially applicable to seeds with mucus on surfaces such as pansy, and particle seeds such as petunia hybrida.

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Owner:SHANGHAI NONGKE GREENING ENG

Planting method for petunia hybrida

InactiveCN104982188APromote growth and developmentEasy to lodgingCultivating equipmentsSoilless cultivationGreenhouseBiology

The invention provides a planting method for petunia hybrida. According to the invention, sowing, cutting or tissue culture propagation is adopted; sowing time is determined according to marketing time, e.g., if flowers are needed in May, greenhouse sowing is carried out in January, and if the flowers are needed in October, sowing is carried out in July; the number of seeds in each gram of seeds is 9000-10000, germination temperature is 20 to 22 DEG C, indoor potted sowing is adopted, and high-temperature sterilized mixed soil of culture soil, rotten leaf soil and fine sand is used; soil covering is not needed after sowing, glass or a transparent plastic plate is pressed on the soil, and germination starts in about 10 days; and when true leaves appear, room temperature is maintained in a range of 13 to 15 DEG C. The planting method for the petunia hybrida provided by the invention enables the petunia hybrida to have good quality.

Owner:LIUZHOU TIANZI HORTICULTURE

Method for producing petunia hybrida vilm potted flower

ActiveCN105706724AQuality improvementStrong growthGrowth substratesCulture mediaGreenhouseEconomic benefits

The invention discloses a method for producing a petunia hybrida vilm potted flower. The method comprises the following steps of preparing a production facility and a culture substrate; seeding; performing seedling stage management; potting; performing potted flower management. A production flow for the petunia hybrida vilm potted flower is developed in the facility, i.e., a greenhouse, and culture management conditions in each flow step are controlled, so that the petunia hybrida vilm potted flower which is high in quality is obtained. The method is simple, feasible, and low in production cost; the production efficiency and the production capacity can be improved; a good economic benefit can be brought; the method is suitable for being popularized and used in production practice; the growth vigor of the produced potted flower is relatively strong; the period from seeding to a flowering period is short; the flowering period is long; the number of flowers is relatively large during the flowering period, and the effect of shielding leaves by the flowers is achieved.

Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Method of parallel screening for insertion mutants and a kit to perform this method

InactiveUS20050059057A1Improve screening efficiencyImprove efficiencyMicrobiological testing/measurementDNA preparationModel systemScreening method

The current invention is a novel approach termed “parallel screening” which allows simultaneously screening of a population for insertions in all genes cloned from that or a closely related organism. In order to test this approach, the flowering plant Petunia hybrida was used as a model system. Petunia hybrida line W137 contains a high copy number of the endogenous transposable element dTph1 and has been previously presented as a genetic tool. A 3D library of the plant genomic DNA of 1000 Petunia hybrida W137 plants was generated. The 3D library consists of 30 pools of DNA from 100 plants each. These were used to generate 30 pools of insertion flanking sequences by nested iPCR using a set of transposon-specific primers or by Transposon Display PCR. Insertions into a gene were detected by hybridizing the amplified insertion flanking sequences fixed to a filter with a gene-specific probe, an approach termed simple screening for insertion elements. Alternatively, the amplified insertion element flanking sequences were labeled and used as a probe to hybridize a filter displaying multiple gene targets, an approach termed parallel screening for insertion elements, which allows the simultaneous screening for insertions in all genes of an organism, appearing in a population of insertion mutants.