The invention relates to a zebrafish vestibular eye movement reflex tester and a test method thereof. The tester includes: a rotating device, including a rotating platform; a zebrafish fixing device, including a body on the rotating platform, the body includes a relative installation surface and a working surface, and the working surface is provided with a fixing groove and an accommodating groove, and the fixing groove It communicates with the holding tank, the fixing tank is used to fix the body of the zebrafish, the holding tank is used to accommodate the head of the zebrafish, and the inner wall of the holding tank is spaced from the head of the zebrafish; the infrared imaging device is located at On the rotating platform, the infrared imaging device includes an infrared light source and an infrared camera, the infrared light source is used to irradiate the head of the zebrafish, and the infrared camera is used to obtain the head image of the zebrafish irradiated by the infrared light source. The zebrafish vestibulo-ocular reflex tester does not require anesthesia of the zebrafish, and can eliminate errors caused by anesthesia used to damage the oculomotor muscles in the vestibulo-ocular reflex test, and has high accuracy.